Characterization and cooling semen curimatã, Prochilodusbrevis (Steindachnner, 1874)

Silva, Angelita Costa da; Galvão, José Agenor Soares; Teixeira, Erivânia Gomes; Farias, Wladimir Ronald Lobo

doi:10.5935/1981-2965.20140121

Cited by 1 publication

(2 citation statements)

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“…This requires an understanding of both male and female gametes to succeed. Although there has been a growing body of research on male gametes, as exemplified by Dreanno et al (1998), Streit Jr et al (2002; Paulino et al (2012), Silva et al (2014), Lenz et al (2018), Caldas and Godoy (2019) and Kowalski and Cejko (2019), very little information is known about the semen of many native species, including the fire-eye tetra, M. oligolepis.…”

Section: Introductionmentioning

confidence: 99%

“…As fish demonstrate a high degree of variability in seminal characteristics (Andrade et al, 2014) and reproductive strategies (Taborsky, 1998), semen analysis is of paramount importance for the performance of artificial fertilization (Murgas et al, 2011). Apart from this, such studies also provide valuable insight into the reproductive biology of native species, and contribute to the establishment of standardized semen conservation protocols (Silva et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Rodrigues

Santos

Cordeiro

et al. 2020

Zygote

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Summary The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

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