Characterization and differentiation of forest species by seed image analysis: a new methodological approach

Abstract

Biometric seed analysis can be used to characterize and differentiate forest species. However, forest species are generally studied using manual methods such as measurements with a digital caliper, which provides a limited amount of information on plant morphological characteristics, whereas agronomic species are analyzed using expensive and often inaccessible equipment. Thus, the objective of the present study was to demonstrate that seed image analysis and processing tools can help characterize and differentiate Brazilian forest species. For this purpose, the seeds of 155 forest species belonging to 42 families were photographed and analyzed to extract data on their morphometric descriptors using a new methodological approach. A total of 18 characteristics were assessed, namely eight dimensions, four shape characteristics, and six color characteristics. A set of approximately 1.827 million data was extracted from 101,521 seed images. Digital image processing efficiently characterized the studied seeds and the obtained characteristics allowed us to differentiate between species, including those belonging to the same botanical family and genus. Therefore, seed image analysis using the proposed methodology can be used to characterize, differentiate, and automatedly identify forest species in Brazil.