Characterization and efficiency of Ganoderma lucidum biomass as an antimicrobial and anticancer agent

Alghonaim, Mohammed Ibrahim; Alsalamah, Sulaiman A.; Alsolami, Ahmed; Abdel Ghany, Tarek M.

doi:10.15376/biores.18.4.8037-8061

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…In the current investigation, the antioxidant activity of C. sempervirens extract may be due to the occurrence of numerous phenolic and flavonoid compounds, as shown via HPLC analysis. Therefore, according to published investigations, a greatly positive association among the phenolic compounds and antioxidant potential seems to be the trend in several species of plants [33][34][35][36]. Flavonoids may capture peroxide, superoxide, and hydroxyl radicals, which may be one of the mechanisms of oxidative damage prevention or repress the proteins responsible for the formation of free radicals.…”

Section: Antioxidant Activity Of C Sempervirens Extractmentioning

confidence: 98%

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Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico

Al-Rajhi,

Bakri,

Qanash

et al. 2023

Molecules

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In the last decade, the urgent need to explore medicinal plants or drug development has increased enormously around the world to overcome numerous health problems. In the present investigation, HPLC indicated the existence of 18 phenolic and flavonoid compounds in the Cupressus sempervirens extract. Hesperetin represents the greatest concentration (25,579.57 µg/mL), while other compounds, such as pyro catechol, rutin, gallic acid, chlorogenic acid, naringenin, and quercetin, were recognized in concentrations of 2922.53 µg/mL, 1313.26 µg/mL, 1107.26 µg/mL, 389.09 µg/mL, 156.53 µg/mL, and 97.56 µg/mL, respectively. The well diffusion method documented the antibacterial/antifungal activity of C. sempervirens extract against E. faecalis, E. coli, C. albicans, S. typhi, S.aureus, and M. circinelloid with 35, 33, 32, 25, 23, and 21 mm inhibition zones, respectively, more than the standard antibiotic/antifungal agent. Low values ranging from 7.80 to 15.62 µg/mL of MIC and MBC were recorded for E. faecalis, E. coli, and C. albicans. From the 1- diphenyl-2-picryl hydrazyl (DPPH) assay, promising antioxidant activity was recorded for C. sempervirens extract with IC50 of an 8.97 µg/mL. Moreover, ferric reducing antioxidant power (FRAP) and total antioxidant capacity assays (TAC) confirmed the antioxidant activity of the extract, which was expressed as the ascorbic acid equivalent (AAE) of 366.9 ± 0.2 µg/mg and 102 ± 0.2 µg/mg of extracts, respectively. α-amylase and α-glucosidase inhibition % were determined to express the antidiabetic activity of the extract in vitro, with promising IC50 value (27.01 µg/mL) for α-amylase compared to that of acarbose (50.93 µg/mL), while IC50 value of the extract for α-glucosidase was 19.21µg/mL compared to that of acarbose 4.13 µg/mL. Prothrombin time (PT) and activated partial thromboplastin time (APTT) revealed the role of C. sempervirens extract as an anticoagulant agent if compared with the activity of heparin. Binding interactions of hesperetin and gallic acid were examined via the Molecular Operating Environment (MOE) Dock software against E. faecalis (PDB ID: 3CLQ), C. albicans (PDB ID: 7RJC), α-amylase (PDB ID: 4W93), and α-glucosidase (PDB ID: 3TOP). The obtained results shed light on how molecular modeling methods might inhibit the tested compounds, which have the potential to be useful in the treatment of target proteins.

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Section: Antioxidant Activity Of C Sempervirens Extractmentioning

confidence: 98%

“…At wavelength 515 nm, the absorption was measured. Ascorbic acid was served as standard [36]. The IC 50 value (the amount requisite to inhibit 50% of the DPPH free radical) of C. sempervirens extract was computed using a log concentration of inhibition curve.…”

Section: Antimicrobial Activity Of C Sempervirens Extractmentioning

confidence: 99%

Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico

Al-Rajhi,

Bakri,

Qanash

et al. 2023

Molecules

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“…While anticancer was performed on colon (Caco2) and prostate (Pc3 cells) (Organism: Homo sapiens Human, Disease: Adenocacinoma). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to detect the cytotoxicity and anticancer activity of ZnO-NPs according to Alghonaim et al (2023) with some modification: The utilized cell lines at 1 × 10 5 cells/mL (100 μL) were inoculated in 96-well containing MTP for 24 h till a complete monolayer sheet was developed. The developmental monolayer sheet had been removed once a consolidated sheet filled with tested cells.…”

Section: Cytotoxic and Anticancer Activity Of Zno-nps Via Mtt Assaymentioning

confidence: 99%

Synthesis of zinc oxide nanoparticles via biomass of Hypnea pannosa as a green mediator and their biological applications

Modafer

2024

BioRes

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The biological manufacturing of zinc oxide nanoparticles (ZnO-NPs) using renewable sources is safe, harmless, and compatible with the environment. The capacity of Hypnea pannosa to synthesize ZnO-NPs was investigated in this work. Ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and zeta potential analysis were employed to characterize the ZnO-NPs. The created ZnO-NPs showed antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Acinetobacter baumannii, Candida albicans, and Candida auris. ZnO-NPs showed an MIC of 12.5 µg/mL against S. aureus, E. faecalis, K. pneumoniae, C. albicans, and C. auris, but it had a 25 µg/mL against Acinetobacter baumannii. ZnO-NPs’ ability to scavenge free radicals was assessed using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) technique with IC50 of 36.2 µg/mL. Anti-inflammatory activity of ZnO-NPs compared to indomethacin at 1000 µg/mL was investigated, where the membrane’s maximum stabilizer was 93.3%. ZnO-NPs demonstrated anticancer activity against PC3 and Caco2 cell lines with IC50 of 174.3 μg/mL and 83.3 μg/mL, respectively. Furthermore, ZnO-NPs demonstrated a range of anti-biofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, ZnO-NPs showed encouraging antiviral effect versus COX B4 as well as HSV1 with antiviral activities of 54.8% and 61.1%, respectively.

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“…Molecular docking (MD) has been extensively applied for virtual drug screening and functional annotation of proteins. It can overcome the limitations of traditional validation experiments (Al-Rajhi et al 2023;Alghonaim et al 2023;Alsalamah et al 2023;Qanash et al 2023). The MD is utilized to predict the binding affinity and compare interactions among laccase with aflatoxins (Liu et al 2020).…”

Section: Introductionmentioning

confidence: 99%

In vitro and molecular docking evaluation of target proteins of lipase and protease for the degradation of aflatoxins

Al-Rajhi,

Ganash,

Alshammari

et al. 2024

BioRes

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The consumption of food contaminated with aflatoxins causes severe harmful health effects, which can lead to death, in both humans and livestock. Therefore, the degradation of aflatoxins, particularly by biological methods, is considered a feasible technology for remediation of aflatoxin-contaminated products. In vitro, aflatoxin B1, B2, G1, and G2 were degraded by 25 U/mL of lipase with reduction percentages of 57.5, 71.7, 80.1, and 83.8%. This reduction increased to 81.3, 82.8, 86.9, and 90.7% via 200 U/mL of lipase, respectively. Protease was less effective than lipase in the degradation of aflatoxin B1, B2, G1, and G2 with reduction levels of 35.8, 54.9, 66.5, and 70.2%, respectively, at 25 U/mL of protease. This investigation offers new concepts for the quick screening of aflatoxin-degrading enzymes and offers a theoretical basis for the degradation of aflatoxins. Interactions between aflatoxin B1 (considered as a ligand) and proteins that were taken as receptors (Structure of Lipase PDB ID: 1DT3 and Protease PDB ID: 2PRO) were elucidated. The molecular modeling results of utilized compound showed a notable binding score and best Root Mean Square (RMS) define value, indicating efficient binding mode and appropriate interactions with amino acids of selected proteins.

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Characterization and efficiency of Ganoderma lucidum biomass as an antimicrobial and anticancer agent

Cited by 4 publications

References 34 publications

Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico

Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico

Synthesis of zinc oxide nanoparticles via biomass of Hypnea pannosa as a green mediator and their biological applications

In vitro and molecular docking evaluation of target proteins of lipase and protease for the degradation of aflatoxins

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