Characterization and Evaluation of the Pro-Coagulant and Immunomodulatory Activities of Polysaccharides from <i>Bletilla striata</i>

Zhai, Wei; Wei, Enwei; Li, Rui; Ji, Tianyi; Yong-ming, Jiang; Wang, Xiaoxiao; Liu, Yiying; Ding, Zhiying; Zhou, Hongli

doi:10.1021/acsomega.0c05171

Cited by 22 publications

(17 citation statements)

References 64 publications

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“…The XPS spectra of STTPs were represented in Figure 6, and selected element signals were S2p, C1s, N1s, and O1s. As shown in Figure 6 A , the wide‐survey XPS spectrum verified the presence of C1s, N1s, and O1s in STTPs at the binding energies of 258.08 eV, 399.08 eV, and 532.08 eV, respectively [42] . C1s and O1s accounted for predominant proportions.…”

Section: Resultsmentioning

confidence: 59%

“…XPS is widely applied to explore the surface elements of tested samples. Recently, this emerging technology has begun to be used for the characterization of polysaccharides [41,42] . In the present study, XPS was performed to characterize STTPs for the first time.…”

Section: Resultsmentioning

confidence: 99%

“…In Figure 6E, the peak at 532.6 eV was assigned to O1s, in particular of the O atom in hydroxy group. [42] The in Vitro Antioxidant Activity of STTPs…”

Section: Partial Characterization Of Sttpsmentioning

confidence: 99%

“…XPS was conducted to reveal the surface elements of purified STTPs by a Physical Electronics PHI 5600 X-ray photoelectron spectrometer (Perkin-Elmer Corporation, Eden Prairie, MN, USA) with a constant pass energy of 20 eV, a takeoff angle of 45°, and an energy step size of 0.05 eV. [42] The in Vitro Antioxidant Activity of STTPs…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

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Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

Zhang

Wang

Yang

et al. 2022

Chemistry & Biodiversity

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Sagittaria trifolia tuber is an aquatic vegetable. In this work, microwave‐assisted enzymatic extraction (MEE) was used to extract S. trifolia tuber polysaccharides (STTPs). Optimum conditions were complex enzyme of 2 %, liquid‐to‐solid ratio of 43 : 1 mL g−1, microwave power of 506 W, and time of 8 min, under which STTPs yield was 36.22±0.69 %, higher than those of other methods. STTPs were sulfated polysaccharides with sulfur valence of S6+. STTPs comprised mannose, glucose, galactose, and arabinose at a mole ratio of 3.69 : 19.33 : 6.21 : 1.00, molecular weights of 3606 kDa and 149.6 kDa, particle size of 220 nm, and zeta potential of −5.02 mV. The surface of STTPs was full of bumps and holes, and abundant in O1s and non‐functionalized C1s. STTPs would scavenge reactive oxygen species with advantage. It would provide an efficient MEE method to obtain antioxidant STTPs, also a clue for extracting polysaccharides from starch‐rich crops.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

“…In Figure 6E, the peak at 532.6 eV was assigned to O1s, in particular of the O atom in hydroxy group. [42] The in Vitro Antioxidant Activity of STTPs…”

Section: Partial Characterization Of Sttpsmentioning

confidence: 99%

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

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Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

Zhang

Wang

Yang

et al. 2022

Chemistry & Biodiversity

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“…We evaluated the activity of PRBS in stimulating macrophages to produce bioactive molecules such as nitric oxide (NO), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL-1β) [ 23 , 24 ]. NO has a wide distribution in various tissues and organs and plays an important role in modulating both innate and adaptive immunity [ 25 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses

Chen

Liu

et al. 2022

J Nanobiotechnol

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Background Nanovaccines have shown the promising potential in controlling and eradicating the threat of infectious diseases worldwide. There has been a great need in developing a versatile strategy to conveniently construct diverse types of nanovaccines and induce potent immune responses. To that end, it is critical for obtaining a potent self-adjuvant platform to assemble with different types of antigens into nanovaccines. Results In this study, we identified a new natural polysaccharide from the rhizomes of Bletilla striata (PRBS), and used this polysaccharide as a platform to construct diverse types of nanovaccines with potent self-adjuvant property. In the construction process of SARS-CoV-2 nanovaccine, PRBS molecules and RBD protein antigens were assembled into ~ 300 nm nanoparticles by hydrogen bond. For HIV nanovaccine, hydrophobic effect dominantly drove the co-assembly between PRBS molecules and Env expression plasmid into ~ 350 nm nanospheres. Importantly, PRBS can potently activate the behaviors and functions of multiple immune cells such as macrophages, B cells and dendritic cells. Depending on PRBS-mediated immune activation, these self-adjuvant nanovaccines can elicit significantly stronger antigen-specific antibody and cellular responses in vivo, in comparison with their corresponding traditional vaccine forms. Moreover, we also revealed the construction models of PRBS-based nanovaccines by analyzing multiple assembly parameters such as bond energy, bond length and interaction sites. Conclusions PRBS, a newly-identified natural polysaccharide which can co-assemble with different types of antigens and activate multiple critical immune cells, has presented a great potential as a versatile platform to develop potent self-adjuvant nanovaccines.

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Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia

Zhang

Liu

et al. 2023

Arabian Journal of Chemistry

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Characterization and Evaluation of the Pro-Coagulant and Immunomodulatory Activities of Polysaccharides from Bletilla striata

Cited by 22 publications

References 64 publications

Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

A new polysaccharide platform constructs self-adjuvant nanovaccines to enhance immune responses

Sulfated modification, basic characterization, antioxidant and anticoagulant potentials of polysaccharide from Sagittaria trifolia

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