Characterization and expression analysis of chalcone synthase and chalcone isomerase genes in Phyllanthus emblica (L.)

Singh, Kashmir; Kumar, Avneesh; Kajal, Monika; Singh, Baljinder

doi:10.1007/s13562-018-0467-5

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…This enzyme (CHI) and chalcone synthase (CHS) cooperate to regulate the synthesis of anthocyanins. CHI participates in the isomerization of chalcone and catalyzes the generation of chalcone to naringenin [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages

Feng,

Tian,

Liang

et al. 2023

BMC Plant Biol

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Background Anthocyanin synthase (ANS) is the enzyme downstream of the anthocyanins synthesis pathway and the rate-limiting enzyme of the synthesis pathway. It catalyzes the conversion of colorless anthocyanins to anthocyanins and plays an important role in plant color presentation and stress resistance. However, ANS gene is rarely studied in grapes. Results In this study, 121 VvANS genes were identified and distributed on 18 chromosomes, VvANS family members were divided into 8 subgroups. Secondary structure prediction showed mainly irregular coils and α-helices, and subcellular localization indicated that VvANS gene family is mainly located in chloroplast, cytoplasm and nucleus. The promoter region of the VvANS gene family contains multiple cis-acting elements that are associated with light, abiotic stress, and hormones. Intraspecific collinearity analysis showed that there were 13 pairs of collinearity between VvANS genes. Interspecific collinearity analysis showed that there was more collinearity between grape, apple and Arabidopsis, but less collinearity between grape and rice. Microarray data analysis showed that VvANS17, VvANS23 and VvANS75 had higher expression levels in flesh and peel, while VvANS25, VvANS64 and VvANS106 had higher expression levels in flower. The results of qRT-PCR analysis showed that VvANS genes were expressed throughout the whole process of fruit coloring, such as VvANS47 and VvANS55 in the green fruit stage, VvANS3, VvANS64 and VvANS90 in the initial fruit color turning stage. The expression levels of VvANS21, VvANS79 and VvANS108 were higher at 50% coloring stage, indicating that these genes play an important role in the fruit coloring process. VvANS4, VvANS66 and VvANS113 had the highest expression levels in the full maturity stage. Conclusions These results indicated that different members of VvANS gene family played a role in different coloring stages, and this study laid a foundation for further research on the function of ANS gene family.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages

Feng,

Tian,

Liang

et al. 2023

BMC Plant Biol

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“…CHI and CHS are considered two decisive structural genes in the flavonoid biosynthesis pathway of Phyllanthus emblica (L.). These genes are closely related to fruit coloration, and the expression pattern increases with fruit development [42]. PgCHS, PgF3H, and PgDFR isolated in pomegranate are highly expressed in red fruits, and their transcript levels show a synchronous increase with the accumulation of flavonoids [43].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Chen

Yang

et al. 2022

IJMS

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Sweet cherries are economically important fruit trees, and their quality changes during development need to be determined. The mechanism of fruit quality changes in sweet cherries were determined by analyzing sweet cherry fruits at 12 developmental stages. The results showed that the soluble sugar, anthocyanin content, and hormones of sweet cherries all changed drastically during the color transition. Therefore, the fruits at the beginning of color conversion, at the end of color conversion, and at the ripening state were selected for the comprehensive analysis of their metabolome and transcriptome. Different sugars, such as D-glucose, sucrose, and trehalose, were identified in the metabolome. Dihydroquercetin, delphinidin-3-glucoside, cyanidin-3-rutincoside, and other flavonoid species were also identified. D-glucose and cyanidin-3-rutinoside were among the most important components of sweet cherry soluble sugars and anthocyanins, respectively. The transcriptional analysis identified key structural genes and nine transcription factors involved in the ABA, sugar, organic acid, and anthocyanin synthesis pathways, with the following specific regulatory patterns. NAC71, WRKY57, and WRKY3 regulate fruit sugar accumulation mainly by acting on INV, SPS, and SUS. MYC2 is involved in the synthesis of anthocyanin precursors by activating PAL and C4H, whereas TCP7 mainly regulates CHI and F3H. WRKY3, NAC71, and WRKY57 have important positive regulatory significance on anthocyanin accumulation, mainly by activating the expression of DFR, ANS, and 3GT.

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“…Anthocyanins, a category of water-soluble pigment, are generated through a branch of the flavonoid pathway and are responsible for the majority of the red, blue, and purple colors seen in plant leaves, fruits, and flowers [1,2]. Besides their role in promoting visual aesthetic appeal in natural and managed landscapes, anthocyanins also protect plants from a range of stresses, such as UV radiation, temperature fluctuations, insects, herbivores, and fungal infections [3,4]. In addition, anthocyanins show many medicinal values regarding human health benefits, especially in preventing cardiovascular diseases, promoting night vision, and alleviating oncogenicity [5,6].…”

Section: Introductionmentioning

confidence: 99%

Cloning, Identification, and Functional Analysis of the Chalcone Isomerase Gene from Astragalus sinicus

Zhang

et al. 2023

Genes

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Astragalus sinicus is an important winter-growing cover crop. It is widely utilized, not only as a cover crop for its benefits in fertilizing the soil but also as a landscape ground cover plant. Anthocyanins are involved in the pigmentation of plants in leaves and flowers, which is a crucial characteristic trait for A. sinicus. The formation of anthocyanins depends significantly on the enzyme chalcone isomerase (CHI). However, research on the CHI gene of A. sinicus remains unexplored. The rapid amplification of cDNA ends (RACE) approach was used in this research to clone the CHI sequence from A. sinicus (AsiCHI). The expression profiles of the AsiCHI gene in multiple tissues of A. sinicus were subsequently examined by qRT-PCR (Quantitative Real-Time PCR). Furthermore, the function of the AsiCHI was identified by the performance of ectopic expression in Arabidopsis (Arabidopsis thaliana). The outcomes revealed that the full-length cDNA of the AsiCHI gene (GeneBank: OQ870547) measured 972 bp in length and included an open reading frame of 660 bp. The encoded protein contains 219 amino acids with a molecular weight of 24.14 kDa and a theoretical isoelectric point of 5.11. In addition, the remarkable similarity between the AsiCHI protein and the CHI proteins of other Astragalus species was demonstrated by the sequence alignment and phylogenetic analysis. Moreover, the highest expression level of AsiCHI was observed in leaves and showed a positive correlation with anthocyanin content. The functional analysis further revealed that the overexpression of AsiCHI enhanced the anthocyanidin accumulation in the transgenic lines. This study provided a better understanding of AsiCHI and elucidated its role in anthocyanin production.

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Characterization and expression analysis of chalcone synthase and chalcone isomerase genes in Phyllanthus emblica (L.)

Cited by 12 publications

References 39 publications

Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages

Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Cloning, Identification, and Functional Analysis of the Chalcone Isomerase Gene from Astragalus sinicus

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