Characterization and expression analysis of EF hand domain-containing calcium-regulatory gene from disk abalone: Calcium homeostasis and its role in immunity

Nikapitiya, Chamilani; Zoysa, Mahanama De; Whang, Ilson; Kim, Se-Jae; Choi, Cheol Young; Lee, Jae‐Seong; Lee, Jehee

doi:10.1016/j.fsi.2010.04.004

Cited by 13 publications

(9 citation statements)

References 49 publications

(41 reference statements)

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“…These calcium binding genes are involved in the maintenance of calcium homeostasis [72]. EF-hand domain containing 2 has been characterized in disc abalone, and the expression analysis indicated it is related to the calcium homeostasis and immune response [73]. Several lectin genes were differentially expressed in some or all groups tested in this study.…”

Section: Discussionmentioning

confidence: 63%

Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Wang

Peatman

et al. 2010

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 63%

Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Wang

Peatman

et al. 2010

Fish & Shellfish Immunology

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“…The members of the EF hand domain family play a pivotal role in calcium regulation within cells and in other physiological processes, including the immune defense mechanism that is activated upon exposure to external stimuli such as pathogens, light and stress [65]. Twenty-two sequences that belong to 14 clusters exhibited homology to EF-hand domain -containing proteins (Table S6).…”

Section: Resultsmentioning

confidence: 99%

Immune-Related Transcriptome of Coptotermes formosanus Shiraki Workers: The Defense Mechanism

Hussain

et al. 2013

PLoS ONE

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Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression Subtractive Hybridization (SSH) libraries were constructed from termite workers infected with entomopathogenic fungi (Metarhizium anisopliae and Beauveria bassiana), Gram-positive Bacillus thuringiensis and Gram-negative Escherichia coli, and the libraries were analyzed. From the high quality normalized cDNA library, 439 immune-related sequences were identified. These sequences were categorized as pattern recognition receptors (47 sequences), signal modulators (52 sequences), signal transducers (137 sequences), effectors (39 sequences) and others (164 sequences). From the SSH libraries, 27, 17, 22 and 15 immune-related genes were identified from each SSH library treated with M. anisopliae, B. bassiana, B. thuringiensis and E. coli, respectively. When the normalized cDNA library was compared with the SSH libraries, 37 immune-related clusters were found in common; 56 clusters were identified in the SSH libraries, and 259 were identified in the normalized cDNA library. The immune-related gene expression pattern was further investigated using quantitative real time PCR (qPCR). Important immune-related genes were characterized, and their potential functions were discussed based on the integrated analysis of the results. We suggest that normalized cDNA and SSH libraries enable us to discover functional genes transcriptome. The results remarkably expand our knowledge about immune-inducible genes in C. formosanus Shiraki and enable the future development of novel control strategies for the management of Formosan subterranean termites.

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“…In bivalves and gastropods, the shell is mainly formed by CaCO 3 crystals. The calcium regulation is an important process for the shell formation, as well as other physiological processes including immune-defense mechanisms (Nikapitiya et al, 2010). Both EF-hand domain-containing protein and ependymin-related protein are involved in calcium homeostasis and have been found to be immune responsive in marine bivalves.…”

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confidence: 99%

A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

et al. 2016

Marine Pollution Bulletin

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The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.

show abstract

Characterization and expression analysis of EF hand domain-containing calcium-regulatory gene from disk abalone: Calcium homeostasis and its role in immunity

Cited by 13 publications

References 49 publications

Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Immune-Related Transcriptome of Coptotermes formosanus Shiraki Workers: The Defense Mechanism

A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

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