Characterization and expression analysis of an interleukin‐7 homologue in the Japanese pufferfish, <i>Takifugu rubripes</i>

Kono, Tomoya; Bird, Steve; Sonoda, Kohei; Savan, Ram; Secombes, Christopher J.; Sakai, Masahiro

doi:10.1111/j.1742-4658.2008.06281.x

Cited by 39 publications

(29 citation statements)

References 53 publications

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“…A fish IL-7 gene that shares limited homology to mammalian IL-7 has been described in fugu. Comparison of the fugu and human genomes shows that some synteny exists around the IL-7 gene, with the presence of both the protein kinase inhibitor-a and chromosome 8 ORF 70 (C8orf70) genes, with IL-7 and C8orf70 having the same transcriptional orientation [14]. Fugu IL-7 shows constitutive expression in HK, spleen, liver, intestine, gill and muscle, with expression shown to increase in HK cultures stimulated with LPS, polyI:C and PHA.…”

Section: Il-7mentioning

confidence: 99%

The cytokine networks of adaptive immunity in fish

Wang

Secombes

2013

Fish & Shellfish Immunology

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290

118

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Section: Il-7mentioning

confidence: 99%

The cytokine networks of adaptive immunity in fish

Wang

Secombes

2013

Fish & Shellfish Immunology

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290

118

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“…With regard to IL-7 signaling, genes encoding homologs of IL-7Ra, TSLPR, and g c chains (24) as well as those encoding the receptor-proximal JAKs have been detected in fish (25). By contrast, it is often difficult to make an unambiguous assignment of presumptive g c cytokines by homology search in genome and expressed sequence tag databases available for lower vertebrates (26), because the primary amino acid sequences of ILs appear to be poorly conserved. Hence, despite its central importance in the immune system of mammals, the role, if any, of IL-7 signaling in lymphocyte development and function of lower vertebrates remains unclear.…”

mentioning

confidence: 99%

Genetic Evidence for an Evolutionarily Conserved Role of IL-7 Signaling in T Cell Development of Zebrafish

Iwanami

Mateos

Hess

et al. 2011

The Journal of Immunology

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In mammals, the cytokine IL-7 is a key regulator of various aspects of lymphocyte differentiation and homeostasis. Because of the difficulty of identifying cytokine homologs in lower vertebrates and the paucity of assay systems and reagents, the degree of functional conservation of cytokine signaling pathways, particularly those pertaining to lymphocyte development, is unclear. In this article, we report on the analysis and characterization of three zebrafish mutants with severely impaired thymopoiesis. The identification of affected genes by positional cloning revealed components of the IL-7 signaling pathway. A presumptive null allele of the zebrafish homolog of the IL-7Rα–chain causes substantially reduced cellularity of the thymus but spares B cell development in the kidney. Likewise, nonsense mutations in the zebrafish homologs of janus kinases JAK1 and JAK3 preferentially affect T cell development. The functional interactions of the cytokine receptor components were examined in the three groups of fish hetero- or homozygous for either il7r and jak1, il7r and jak3, or jak1 and jak3 mutations. The differential effects on T cell development arising from the different genotypes could be explained on the basis of the known structure of the mammalian IL-7R complex. Because IL-7 signaling appears to be a universal requirement for T cell development in vertebrates, the mutants described in this article represent alternative animal models of human immunodeficiency syndromes amenable to large-scale genetic and chemical screens.

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“…Fugu IL-7 shows constitutive expression in head kidney, spleen, liver, intestine, gills and muscle, with expression shown to increase in head kidney cultures stimulated with LPS, poly I:C or PHA [25].…”

Section: Il-7mentioning

confidence: 98%

“…The organisation of the fugu IL-7 gene is the same as in mouse, and consists of five exons and four introns but differs from the human gene where six exons and five introns are present. Comparison of the Fugu and human genomes shows that some synteny exists around the IL-7 gene, with the presence of both the protein kinase inhibitor-a and chromosome 8 ORF 70 (C8orf70) genes, with IL-7 and C8orf70 having the same transcriptional orientation [25].…”

Section: Il-7mentioning

confidence: 99%