Characterization and Expression of a cDNA Encoding a Seed-Specific Metallothionein in Maize

White, Constance; Rivin, Carol J.

doi:10.1104/pp.108.2.831

Cited by 41 publications

(29 citation statements)

References 10 publications

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“…Type 4 MT genes contain promoter sequences with homology to ABAresponse elements, and their expression is regulated by ABA (83). These genes follow the same regulatory program as a large number of other genes that are expressed during the maturation of embryos and whose RNAs persist until imbibition and germination.…”

Section: Mt Gene Expressionmentioning

confidence: 99%

PHYTOCHELATINS AND METALLOTHIONEINS: Roles in Heavy Metal Detoxification and Homeostasis

Cobbett

Goldsbrough

2002

Annu. Rev. Plant Biol.

2,274

1,492

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Section: Mt Gene Expressionmentioning

confidence: 99%

PHYTOCHELATINS AND METALLOTHIONEINS: Roles in Heavy Metal Detoxification and Homeostasis

Cobbett

Goldsbrough

2002

Annu. Rev. Plant Biol.

2,274

1,492

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“…6,7) In contrast with the other three types of MT-like proteins that comprise two cysteine-rich segments, the Ec type of proteins possess three cysteine-rich segments with their cysteine residues highly conserved. [18][19][20] The expression of plant Ec type of MT-like genes has so far been found only during pollen germination or seed maturation. Similarly, the SiMT transcript was detected in maturing seeds, but not in the root, stem, leaves, capsules, or flowers.…”

Section: Discussionmentioning

confidence: 99%

“…15,16) The transcript of type 3 MT-like proteins has been found in leaves, fruits and developing embryos, 17,18) while the transcript of Ec type has been found to be restricted to plant reproductive organs. [19][20][21] To date, only a limited number of plant Ec type MT-like genes have been isolated and characterized in detail. 20,21) An SiMT gene encoding an Ec type of MT-like protein in sesame seeds was cloned in this study, and its tissuespecific expression characterized.…”

Section: -3)mentioning

confidence: 99%

“…[19][20][21] To date, only a limited number of plant Ec type MT-like genes have been isolated and characterized in detail. 20,21) An SiMT gene encoding an Ec type of MT-like protein in sesame seeds was cloned in this study, and its tissuespecific expression characterized. A recombinant GSTSiMT fusion protein was over-expressed in Escherichia coli, and its capability for binding to metal ions was examined.…”

Section: -3)mentioning

confidence: 99%

“…Similarly low copy numbers of Ec genes have been found in wheat and maize genomes. 19,20) Bacterial expression of SiMT The SiMT cDNA fragment was constructed into an Nterminal GST fusion vector (pGEX-3X) and transformed into E. coli. The expression of the GST-SiMT fusion protein was monitored by western blotting, using antibodies against GST (Fig.…”

Section: Genomic Cloning Of Simt and Its Organization In The Sesame Gmentioning

confidence: 99%

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Cloning and Expression of a Seed-Specific Metallothionein-Like Protein from Sesame

Chyan

Lee

Liu

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet

et al. 2004

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A pot trial was conducted with 52 rice cultivars of different types collected from different origins. The results showed that there were great differences in Cd concentrations in straw, brown rice and grain chaff among the rice cultivars grown in a soil containing a Cd concentration of 100 mg kg −1 ; the Cd concentrations in brown rice ranged from 0.22 to 2.86 mg kg −1 . The great genotypic differences in Cd concentrations indicated that it is possible to lower the Cd content of rice through cultivar selection and breeding. Significant differences were found in the Cd concentrations of the rice types of Indica consanguinity and those of Japonica consanguinity, but not between their origins. There were significant correlations between straw and brown rice in Cd concentration and in the total amount of Cd accumulated. The distribution ratios of the Cd accumulated in brown rice to the total Cd accumulation in the aboveground rice plant varied greatly from 12.9 to 137.8 g kg −1 , and there was significant correlation between the distribution ratios and Cd concentrations in brown rice. These indicated that Cd concentration in rice grain is governed by the transport of Cd from root to shoot and also from shoot to grain. Cd concentrations in brown rice also correlated significantly with some important agronomic traits, as well as with nitrogen concentrations, one of the most important criteria for rice quality.

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Characterization and Expression of a cDNA Encoding a Seed-Specific Metallothionein in Maize

Cited by 41 publications

References 10 publications

PHYTOCHELATINS AND METALLOTHIONEINS: Roles in Heavy Metal Detoxification and Homeostasis

PHYTOCHELATINS AND METALLOTHIONEINS: Roles in Heavy Metal Detoxification and Homeostasis

Cloning and Expression of a Seed-Specific Metallothionein-Like Protein from Sesame

Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet

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