Characterization and expression profile of CaNAC2 pepper gene

Guo, Wei-Li; Wang, Shubin; Chen, Rugang; Chen, Bihua; Du, Xiaohua; Yin, Yanxu; Gong, Zhen-Hui; Zhang, Yuyuan

doi:10.3389/fpls.2015.00755

Cited by 49 publications

(28 citation statements)

References 41 publications

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“…A report on aubergine (Solanum melongena) NAC (SmNAC) transcription factor expression by Na et al (2016) indicates that SmNAC is induced by Ralstonia solanacearum infection and MeJA treatment, and SmNAC overexpression resulted in the reduced accumulation of SA which increased bacterial wilt susceptibility of aubergine. Published information on the functional characterization of chili pepper and aubergine NAC transcription factors are so scanty (Oh et al 2005;Guo et al 2015) and more study in this area need to be conducted.…”

Section: Roles Of Nac Transcription Factors In Tobacco (Nicotiana Tabmentioning

confidence: 99%

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

Tweneboah

2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Evolutionary studies conducted on NAC (NAM, ATAF1&2, and CUC2) genes for all major groups of land plants, indicate the presence of the NAC subfamilies, even in the early land plants. The varied roles played by NAC proteins in plant growth and development range from the formation of shoot apical meristem, floral organ development, reproduction, lateral shoot development, and defense responses to biotic and abiotic stresses. Considering the value and importance of solanaceous crops, the study of NAC proteins in these plants needs to be intensified. This will help to identify and functionally characterize their promoters, which will subsequently aid in engineering plants with improved performance under stressful conditions. In this review, the functionally characterized NAC transcription factors specific to tomato, potato, tobacco, chili pepper and eggplant (aubergine) are summarized, clearly indicating their biological functions in the defense mechanism of the plants, against biotic and abiotic stresses.

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Section: Roles Of Nac Transcription Factors In Tobacco (Nicotiana Tabmentioning

confidence: 99%

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

Tweneboah

2017

J Plant Biotechnol

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“…Various abiotic stresses such as temperature extremes, drought, and high salinity, are major factors that influence the distribution, growth, and development of pepper plants. Therefore, it is important to investigate the mechanisms underlying resistance or tolerance in pepper to the adverse environmental conditions (Guo et al, 2015). In this study, a NAC transcription factor gene (CaNAC035) was isolated from pepper line 'P70' leaves.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)

Zhang

Wang

et al. 2020

Front. Plant Sci.

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NAC (NAM, ATAF1/2, and CUC2) proteins are the plant-specific transcription factors (TFs) which are important in plant response to abiotic stresses. However, knowledge about the functional role that NACs play in pepper abiotic stress tolerance is limited. In this study, we isolated a NAC TF gene, CaNAC035, from pepper (Capsicum annuum L.), where the protein is localized in the nucleus and functions as a transcriptional activator. CaNAC035 expression is induced by low and high temperatures, osmotic stress, salt, gibberellic acid (GA), methyl-jasmonic acid (MeJA), salicylic acid (SA), and abscisic acid (ABA). To understand the function of CaNAC035 in the abiotic stress responsep, we used virusinduced gene silencing in pepper to knockdown the CaNAC035 and overexpressed the CaNAC035 in Arabidopsis. The results showed that pepper seedlings in which CaNAC035 was silenced, showed more damage than the control pepper plants after cold, NaCl, and mannitol treatments. Correspondingly increased electrolyte leakage, a higher level of malondialdehyde (MDA), H 2 O 2 , and superoxide radicals were found after cold treatments. CaNAC035-silenced seedlings exhibited lower chlorophyll content while CaNAC035-overexpressed Arabidopsis plants had higher germination rate and fresh weight after mannitol and NaCl treatments. We also reported 18 proteins that potentially interact with CaNAC035 and may participate in processes such as the stress response, resistance, and photosynthesis. Our results suggest that CaNAC035 is a positive regulator of abiotic stress tolerance in pepper which acts through multiple signaling pathways.

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“…The RNA extraction, first-strand cDNA synthesis and qRT-PCR were completed as described by Guo et al (2015). Relative gene expression levels were determined with the 2 -DDCT method.…”

Section: Qrt-pcr Analysismentioning

confidence: 99%

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

et al. 2020

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Powdery mildew (PM), caused by Podosphaera xanthii, is a major threat to the global cucurbit yield. The molecular mechanisms underlying the PM resistance of pumpkin (Cucurbita moschata Duch.) are largely unknown. A homolog of the basic helix-loop-helix (bHLH) transcription factor was previously identified through a transcriptomic analysis of a PM-resistant pumpkin. In this study, this bHLH homolog in pumpkin has been functionally characterized. CmbHLH87 is present in the nucleus. CmbHLH87 expression in the PMresistant material was considerably downregulated by PM; and abscisic acid, methyl jasmonate, ethephon, and NaCl treatments induced CmbHLH87 expression. Ectopic expression of CmbHLH87 in tobacco plants alleviated the PM symptoms on the leaves, accelerated cell necrosis, and enhanced H 2 O 2 accumulation. The expression levels of PR1a, PR5, and NPR1 were higher in the PM-infected transgenic plants than in PMinfected wild-type plants. Additionally, the chlorosis and yellowing of plant materials were less extensive and the concentration of bacteria at infection sites was lower in the transgenic tobacco plants than in the wild-type plants in response to bacterial wilt and scab pathogens. CmbHLH87 may be useful for genetic engineering of novel pumpkin cultivars in the future.

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Characterization and expression profile of CaNAC2 pepper gene

Cited by 49 publications

References 41 publications

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)

Expression of Pumpkin CmbHLH87 Gene Improves Powdery Mildew Resistance in Tobacco

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