Characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (<i>Oryza sativa</i> L.)

Abstract

Chlorophyll content is one of the most important traits controlling crop biomass and economic yield in rice. Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage. At temperatures below 22°C, the tscd1 mutant showed the most obvious yellowish phenotype. With increasing temperature, the yellowish leaves gradually turned green and approached a normal wild type color. Wild type and tscd1 mutant plants had obviously different chloroplast structures and photosynthetic pigment precursor contents, which resulted in underdevelopment of chloroplasts and a yellowish phenotype in tscd1. Genetic analysis indicated that the mutant character was controlled by a recessive nuclear gene. Through map-based cloning, we located the tscd1 gene in a 34.95 kb region on the long arm of chromosome 2, containing two BAC clones and eight predicted candidate genes. Further characterization of the tscd1 gene is underway. Because it has a chlorophyll deficit phenotype before the tiller stage and little influence on growth vigor, it may play a role in ensuring the purity of hybrids.

Mixed‐cropping systems of different rice cultivars have grain yield and quality advantages over mono‐cropping systems

“…Enhanced photosynthetic rate and SPAD index might lead to the improved performance of rice mixtures. Chlorophyll content (expressed as SPAD index), is one of the most important factors associated with photosynthetic rate, as well as crop biomass and economic yield in rice . Enhanced photosynthetic rate even at the single‐leaf level has recently been found to be a significant contributor in improving crop productivity .…”

“…Chlorophyll content (expressed as SPAD index), is one of the most important factors associated with photosynthetic rate, as well as crop biomass and economic yield in rice. 35 Enhanced photosynthetic rate even at the single-leaf level has recently been found to be a significant contributor in improving crop productivity. 36,37 Furthermore, positive correlations between the photosynthetic rate, SPAD index, total aboveground dry weight and the grain weight per pot in the early and late growing seasons were also observed at maturity.…”

Cytogenetic Analyses of PSL1 Mutant, a Novel Low‐Temperature‐Sensitive Purple‐Striped Leaf Color Mutant in Wheat

“…There are two known types of low-temperature-sensitive leaf color mutants in plants. The characteristics of these low-temperature-sensitive leaf color mutants are generally controlled by single recessive nuclear genes (Tan et al, 2014;Liu et al, 2015). The characteristics of these low-temperature-sensitive leaf color mutants are generally controlled by single recessive nuclear genes (Tan et al, 2014;Liu et al, 2015).…”

“…One type is sensitive to a restrictive temperature of ?20°C, such as many tropical and subtropical plants including rice (Kusumi and Iba, 2014). The characteristics of these low-temperature-sensitive leaf color mutants are generally controlled by single recessive nuclear genes (Tan et al, 2014;Liu et al, 2015). In rice, tscd1 produces obvious yellow leaves in the seedling stage at temperatures below 22°C, but they gradually turn green after the booting stage when the temperature increases (Liu et al, 2015).…”

“…The characteristics of these low-temperature-sensitive leaf color mutants are generally controlled by single recessive nuclear genes (Tan et al, 2014;Liu et al, 2015). In rice, tscd1 produces obvious yellow leaves in the seedling stage at temperatures below 22°C, but they gradually turn green after the booting stage when the temperature increases (Liu et al, 2015). The mutant's characteristics of the tscd1 are controlled by a recessive nuclear gene.…”