Characterization and function of a cathepsin B in red crayfish ( Procambarus clarkii ) following lipopolysaccharide challenge

Dai, Li-Shang; Sun, Yu; Sun, Yuxuan; Zhu, Bing; Liu, Chao-Liang

doi:10.1016/j.fsi.2016.07.016

Cited by 23 publications

(2 citation statements)

References 31 publications

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“…The cathepsin family of proteins plays a pivotal role in lysosomal proteolysis, which is central to the degradation and recycling of proteins within cells [37]. Their activity is highly dependent on the acidic environment of the lysosome, maintained by the V-ATPase complex that includes ATP6N [38]. The observed downregulation of cathepsin genes, in conjunction with the downregulation of ATP6N, implies a potential disruption in lysosomal proteolysis.…”

Section: Analysis Of Lysosome Pathwaymentioning

confidence: 99%

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Zhang,

Wang,

Liu

et al. 2023

Water

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In agricultural and fishery production, the occurrence of cyanobacterial blooms and the contamination of freshwater systems with microplastics have become increasingly important research focuses. However, the individual and combined toxic mechanisms of these two pollutants are not yet fully understood. Therefore, in this study, we analyzed the effects of these two stressors, microcystins (MC) and nanoplastics (NP), on the transcriptome and metabolome of the hepatopancreas of river clams. RNA and metabolites extracted from river clams treated with MC, NP, and a combination of MC and NP were used to construct standardized cDNA libraries, which were then subjected to integrated analysis. Significant enrichment of 49 pathways, 34 pathways, and 44 pathways was observed in the MC group compared to the control group, NP group compared to the control group, and NP-MC group compared to the control group, respectively. In these three experimental groups, we found that the lysosome pathway, which affects immune function and cell apoptosis, was enriched with numerous differentially expressed genes and metabolites. Changes in ATP6N and ADP may impair lysosomal acidification and disrupt normal lysosomal degradation processes, indicating interference with the hepatopancreatic metabolism of pollutants. Interestingly, we observed significant alterations in the cathepsin family, and the downregulation of cathepsin genes, along with the downregulation of ATP6N, implies a potential disruption in lysosomal proteolysis. In the NP-MC group, the downregulation of purine expression levels suggests an impact on the immune system of river clams by NP-MC. In conclusion, while there is some overlap in the damage caused to the hepatopancreas of river clams by MCs, MPs, and the combination of NP-MC, further research is necessary to fully understand their effects.

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Section: Analysis Of Lysosome Pathwaymentioning

confidence: 99%

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Zhang,

Wang,

Liu

et al. 2023

Water

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“…These enzymes can be used as indexes to evaluate nonspecific immunity as well [ 9 , 10 ]. The enzymes AKP and ACP regulate metabolism in crustaceans and play an essential role in improving disease resistance and substance metabolism [ 9 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Sources of Culture Substrate on the Growth and Immune Performance of the Red Swamp Crayfish (Procambarus clarkii)

Wan,

Zhang,

Tang

et al. 2023

IJMS

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The substrate in the aquatic environment plays a crucial role in nutrient deposition and recovery for the growth of aquatic organisms. In order to optimize the culture medium of Procambarus Clarkii, culture media from different sources were selected in this study to explore their effects on the growth and immune performance of red swamp crayfish. The results showed that the weight gain rate (WGR), body length growth rate (BLGR) and specific growth rate (SGR) in group I2 were the highest, followed by group I1 and group I3. The WGR and SGR of crayfish in the I1 and I2 groups were significantly higher than those in the I3 group (p < 0.05). The activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and superoxide dismutase (SOD) were the highest in group I2, followed by group I3, and the lowest in group I1. The expression trends in growth-related genes, nuclear hormone receptor (E75), molt-inhibiting hormone (MIH) and chitinase genes were similar, and the expression levels in the I2 group were higher than those in the I1 and I3 groups. It was noted that the expression levels of E75 and MIH genes in the I2 group were significantly higher than those in the I3 group (p < 0.05). α diversity analysis of 16S rRNA data showed that there was no statistically significant difference in the abundance of intestinal flora among the three culture substrate groups. The β diversity in the Xitangni group, crayfish Tangni group and Shuitangni group was significantly different. These changes in microbiota suggest that using different substrates to culture crayfish leads to differences in gut microbiota diversity. To sum up, the growth in crayfish and immune performance influenced by the culture substrate condition and aquatic breeding sediment substrates, rather than crab pool and paddy field pond sediment substrates, showed a better effect.

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Pradhan

Padhi

Dash³

et al. 2022

Nutraceuticals and Health Care

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Characterization and function of a cathepsin B in red crayfish ( Procambarus clarkii ) following lipopolysaccharide challenge

Cited by 23 publications

References 31 publications

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Effects of Different Sources of Culture Substrate on the Growth and Immune Performance of the Red Swamp Crayfish (Procambarus clarkii)

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