Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche

CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche

“…Although these changes were significant, they were not substantial suggesting that reduced signaling through HoxB4 and N-cadherin may play a role in the altered function of HSPC from KO mice and the diminished competence of the CD166 2/2 HN. Expression levels of fibulins, which negatively influence the adhesive properties of the endosteal niche, 32 were significantly increased in KO OB (supplemental Figure 8D), suggesting that loss of CD166 upregulates the expression of fibulins and indirectly reduces the adhesive properties of OB, possibly through increased expression of MMP2, a pathway known to be triggered by CD166. 33 …”

The integrin 9 1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation

“…Since human osteoblasts synthesize and express VCAM-1 and tenascin-C, 31,33 other ligands for α9β1 on HSPC such as ADAM proteases, thrombospondin-1 or fibulin-5 might be responsible for the observed α9β1-mediated adhesion. 23,34,35 On the other hand, α9β1 is also expressed by human osteoblasts and could, therefore, interact with adhesive ligands present on HSPC.…”

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

“…G292 and Mg-63 cells were grown in DMEM (4.5 g/l glucose, L-glutamine; Invitrogen) with 10% FCS (Invitrogen) and 1% penicillin/ streptomycin (Invitrogen). Primary human osteoblasts were isolated and cultured as described previously (Hergeth et al, 2008).…”