Characterization and functional role of leptin receptor in bovine adrenal medullary cells

Yanagihara, Nobuyuki; Utsunomiya, Kensuke; Cheah, Tat Beng; Hirano, Hideyasu; Kajiwara, Koji; Hara, Koji; Nakamura, Eiichiro; Toyohira, Yumiko; Uezono, Yasuhito; Ueno, S.; Izumi, Fujio

doi:10.1016/s0006-2952(00)00240-9

Cited by 29 publications

(35 citation statements)

References 21 publications

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“…The tissue distribution of leptin receptor isoforms in bovine is not well established. The expression of Ob-Ra has been reported previously only in the adrenal gland [17], pituitary gland, liver and spleen in cattle [18], and in the lactating ovine mammary gland [19]. Previously we demonstrated that leptin alone had no effect on alpha casein gene expression and fatty acid synthesis in bovine mammary explants, nonetheless in the presence of prolactin, leptin up-regulated alpha casein and beta lactoglobulin expression, and fatty acid synthesis [20].…”

Section: Introductionmentioning

confidence: 99%

Mammary Fat Can Adjust Prolactin Effect on Mammary Epithelial Cells via Leptin and Estrogen

Feuermann

Mabjeesh

Shamay

2009

International Journal of Endocrinology

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Leptin, like estrogen, is one of the endo/paracrine factors, which are synthesized in and secreted from mature adipocytes. The roles of the mammary fat pad and mammary adipocytes in the initiation of lactation are not clear. In this study, we showed that combination of prolactin, leptin and estrogen elevated the expression of the milk protein beta-lactoglobulin. We also showed that after prolactin stimulate the secretion of leptin from the mammary fat, leptin upregulated the expression of estrogen receptor alpha in the mammary epithelial cells. Also, prolactin affected aromatase mRNA expression in the bovine mammary fat and we demonstrated that leptin and prolactin can affect cholesterol secretion from explants in culture to the medium. Therefore, we suggest that prolactin initiates estrogen expression (as represented by aromatase mRNA) in the mammary fat pad, whereas leptin stimulates estrogen receptor alpha expression in the mammary epithelial cells. We hypothesize that leptin and estrogen, secreted from the mammary fat regulate lactation after stimulation of prolactin.

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Section: Introductionmentioning

confidence: 99%

Mammary Fat Can Adjust Prolactin Effect on Mammary Epithelial Cells via Leptin and Estrogen

Feuermann

Mabjeesh

Shamay

2009

International Journal of Endocrinology

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“…In prepubertal heifers (Silva et al, 2002) it was reported that the long isoform of leptin receptor mRNA was present in all bovine tissues, while the short isoform (ObRa) was detected only in bovine liver, pituitary gland, and spleen. Reverse transcription-polymerase chain reaction (RT-PCR) studies indicate the expression of the short (Ob-Ra) isoform in the bovine adrenal medulla (Yanagihara et al, 2000). Both long (Ob-Rb) and short (Ob-Ra) isoforms of leptin receptor were found in ovine mammary tissue during pregnancy and lactation (Laud et al, 1999).…”

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confidence: 99%

Expression and localisation of leptin and leptin receptor in the mammary gland of the dry and lactating non-pregnant cow

Sayed-Ahmed

Kulcsár

Rudas

et al. 2004

Acta Veterinaria Hungarica

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Leptin and leptin receptor were studied in the mammary gland of nonpregnant dry and lactating cows. Using RT-PCR it was demonstrated that leptin and its short (Ob-Ra) and long (Ob-Rb) receptor isoforms are expressed both in the dry and the lactating mammary gland tissue. Tissue distribution of leptin and its receptor mRNA transcripts were examined by in situ hybridisation, while the leptin protein was localised by immunohistochemistry. Although in situ hybridisation is semiquantitative, our morphological data suggest that the epithelial leptin mRNA expression of the lactating gland is higher than that of the dry gland. To compare the leptin mRNA levels between dry and lactating udders competitive PCR was used, which showed no difference in leptin expression for the whole mammary tissues. The lack of difference in total leptin mRNA levels is explained by the high adipose tissue content of the dry mammary gland. Leptin and its receptor transcripts are expressed mainly in the epithelial cells of lactating cows, while in dry mammary tissue the signal is found in the stromal tissues as well. The results provide additional evidence that locally produced leptin takes part in the regulation and maintenance of mammary epithelial cell activity.

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“…The increase of cytosolic Ca 2ϩ is a limiting factor for catecholamine secretion in chromaffin cells (26). However, leptin did not affect catecholamine release in adrenomedullary cells of humans (24) and bovines (57). The lower expression of OBR and JAK2 could explain the lower stimulatory effect of leptin on catecholamine secretion in the programmed animals.…”

Section: E947mentioning

confidence: 98%

“…There are few reports about the relationship between leptin and adrenal catecholamines; most of them were performed in cultured cells. It was shown that leptin stimulates the function of adrenal chromaffin cells through activation of catecholamine synthesis and secretion (49,57). However, the intracellular mechanisms involved in OBR signaling in adrenal medulla of rats are still unclear, whereas it is well recognized that leptin stimulates the sympathetic nervous system (SNS), which may result in adverse changes in cardiovascular parameters (41).…”

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confidence: 99%

Programming of rat adrenal medulla by neonatal hyperleptinemia: adrenal morphology, catecholamine secretion, and leptin signaling pathway

Trevenzoli¹,

Pinheiro²,

Conceição³

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

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Trevenzoli IH, Pinheiro CR, Conceição EP, Oliveira E, Passos MC, Lisboa PC, Moura EG. Programming of rat adrenal medulla by neonatal hyperleptinemia: adrenal morphology, catecholamine secretion, and leptin signaling pathway. Am J Physiol Endocrinol Metab 298: E941-E949, 2010. First published February 9, 2010 doi:10.1152/ajpendo.00734.2009.-Leptin serum concentration in early life is an important factor for adequate future development of the offspring. Previously, we demonstrated that hyperleptinemia on lactation programmed for hyperleptinemia, central leptin resistance with lower expression of the long form of leptin receptor at hypothalamus, and higher medullary catecholamine levels with cardiovascular consequences at adulthood. The central objective of this study was to determine the direct effect of leptin on adrenal medullary function of adult rats that were leptin treated during lactation. Adrenal morphology was also accessed. Recombinant murine leptin was injected in the pups during the first 10 days of life (group L, leptin-programmed) or at adulthood during 6 days (group LC). The controls of both experiments received saline (groups C and CC). Both treatments resulted in hyperleptinemia at 150 days old (ϩ78% and 2-fold increase, respectively; P Ͻ 0.05). Programmed animals showed hypertrophy of adrenal and higher adrenal catecholamine content at 150 days old (3-fold increase, P Ͻ 0.05), and no changes were observed in the LC group. However, LC rats had lower adrenal content of tyrosine hydroxylase (Ϫ17%, P Ͻ 0.05). Leptin-programmed rats had a lower response to leptin in vitro stimulation (Ϫ22%, P Ͻ 0.05) and lower expression of key proteins of the leptin signaling pathway, leptin receptor and janus tyrosine kinase 2 in the medullas (Ϫ61% and Ϫ29%, respectively, P Ͻ 0.05). However, they presented higher expression of phosphorylated signal transducer and activator of transcription 3 (ϩ2-fold, P Ͻ 0.05). Leptin treatment at adulthood did not affect these parameters. The higher catecholamine synthesis and secretion in the leptin-programmed rats observed in our previous study does not seem to be a consequence of the direct effect of leptin on the medullas. We suggest that the hyperleptinemia of the programmed animals increases adrenal medullary function through sympathetic nervous system activation. In conclusion, high leptin levels on lactation program the activity of the sympathoadrenal system at adulthood that may contribute to the development of adult chronic diseases such as hypertension.

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Characterization and functional role of leptin receptor in bovine adrenal medullary cells

Cited by 29 publications

References 21 publications

Mammary Fat Can Adjust Prolactin Effect on Mammary Epithelial Cells via Leptin and Estrogen

Mammary Fat Can Adjust Prolactin Effect on Mammary Epithelial Cells via Leptin and Estrogen

Expression and localisation of leptin and leptin receptor in the mammary gland of the dry and lactating non-pregnant cow

Programming of rat adrenal medulla by neonatal hyperleptinemia: adrenal morphology, catecholamine secretion, and leptin signaling pathway

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