Characterization and gene expression of heat shock protein 90 in marine crab &amp;lt;i&amp;gt;Charybdis japonica&amp;lt;/i&amp;gt; following bisphenol A and 4-nonylphenol exposures

Park, Kiyun

doi:10.5620/eht.2014.29.e2014002

Cited by 16 publications

(8 citation statements)

References 39 publications

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“…Twelve fish were assessed at each estrogen sampling point. All chemical concentrations were based on previous reports (Atli & Canli, ; Cho et al., ; Park & Kwak, ; Tong et al., ). Fish were sacrificed, and kmWap65‐1 and kmWap65‐2 mRNA patterns were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Identification of warm temperature acclimation‐associated 65‐kDa protein‐2 in Kumgang fat minnow Rhynchocypris kumgangensis

Kwon

Ghil

2017

J Exp Zool Pt A

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Warm temperature acclimation-associated 65-kDa protein (Wap65) is known to respond to elevated water temperatures and the corresponding gene from several fish species has been cloned. Expression of Wap65 gene is induced by various physiological stresses, such as increase in water temperature, immune response and heavy metal exposure. Two isolated Wap65 genes, Wap65-1 and Wap65-2, display distinct tissue distribution and physiological functions despite high sequence homology. In a previous study, we identified the Wap65-1 gene (kmWap65-1) from Kumgang fat minnow, Rhynchocypris kumgangensis, a small freshwater fish endemic to Korea. The kmWap65-1 gene showed sequence homology with teleost Wap65-1 and mammalian hemopexin, and was highly expressed in response to increased water temperature and bacterial lipopolysaccharide (LPS) exposure. Here, we isolated kmWap65-2 from liver tissue of Kumgang fat minnow and compared the expression profiles of both kmWap65 genes following exposure to various physiological stresses, including thermal changes, bacterial challenge, and environmental toxins. Notably, while kmWap65-1 expression was significantly increased in response to high water temperature, LPS, cadmium, and iron, kmWap65-2 displayed no alterations in expression at high water temperature. However, kmWap65-2 expression was upregulated slightly in response to LPS and highly in presence of copper, bisphenol A, and estradiol. Based on the collective findings, we propose that kmWap65-1 and kmWap65-2 are multifunctional proteins with distinct functions that could serve as useful biomarkers for assessing physiological stress and associated responses in Kumgang fat minnow.

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Section: Methodsmentioning

confidence: 99%

Identification of warm temperature acclimation‐associated 65‐kDa protein‐2 in Kumgang fat minnow Rhynchocypris kumgangensis

Kwon

Ghil

2017

J Exp Zool Pt A

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“…Several studies show that HSP genes are upregulated in response to various stresses. For instance, Alexandre et al (2001) (3) found that HSP genes were upregulated in response to ethanol stress, while HSP70 and HSP90 were significantly induced in C. japonica crabs exposed to different inhibitory concentrations of NP (86,87). Furthermore, several stress genes, including HSP12 and HSP26, were significantly upregulated in yeast cells exposed to cadmium (76).…”

Section: High Inhibitory Concentration Of Np Upregulates Genes Encodimentioning

confidence: 99%

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Bereketoğlu

Arğa

Eraslan

et al. 2017

Physiological Genomics

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Bioaccumulative environmental estrogen, nonylphenol (NP; 4-nonylphenol), is widely used as a nonionic surfactant and can affect human health. Since genomes of and higher eukaryotes share many structural and functional similarities, we investigated subcellular effects of NP on BY4742 cells by analyzing genome-wide transcriptional profiles. We examined effects of low (1 mg/l; <15% cell number reduction) and high (5 mg/l; >65% cell number reduction) inhibitory concentration exposures for 120 or 180 min. After 120 and 180 min of 1 mg/l NP exposure, 187 (63 downregulated, 124 upregulated) and 103 genes (56 downregulated, 47 upregulated), respectively, were differentially expressed. Similarly, 678 (168 repressed, 510 induced) and 688 genes (215 repressed, 473 induced) were differentially expressed in cells exposed to 5 mg/l NP for 120 and 180 min, respectively. Only 15 downregulated and 63 upregulated genes were common between low and high NP inhibitory concentration exposure for 120 min, whereas 16 downregulated and 31 upregulated genes were common after the 180-min exposure. Several processes/pathways were prominently affected by either low or high inhibitory concentration exposure, while certain processes were affected by both inhibitory concentrations, including ion transport, response to chemicals, transmembrane transport, cellular amino acids, and carbohydrate metabolism. While minimal expression changes were observed with low inhibitory concentration exposure, 5 mg/l NP treatment induced substantial expression changes in genes involved in oxidative phosphorylation, cell wall biogenesis, ribosomal biogenesis, and RNA processing, and encoding heat shock proteins and ubiquitin-conjugating enzymes. Collectively, these results provide considerable information on effects of NP at the molecular level.

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“…BPA concentrations observed in U.S. river estuaries were approximately 4 µg L −1 to 21 µg L −1 [12,13]. Exposure to BPA induced transcription responses of the heat shock protein 90 (HSP90) gene in the marine crab Charybdis japonica [14]. Di-(2-ethylhexyl) phthalate (DEHP), a suspected EDC, is widely used as plasticizer and may induce reproductive and developmental toxicities in aquatic environments [15].…”

Section: Introductionmentioning

confidence: 99%

Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

Park

Kim

et al. 2019

Applied Sciences

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Vitellogenesis is a pivotal reproductive process of the yolk formation in crustaceans. Vitellogenin (VTG) is the precursor of main yolk proteins and synthesized by endogenous estrogens. The intertidal mud crab (Macrophthalmus japonicus) inhabits sediment and is a good indicator for assessing polluted benthic environments. The purpose of this study was to identify potential responses of M. japonicus VTG under environmental stresses caused by chemical pollutants, such as 1, 10, and 30 µg L−1 concentrations in di(2-ethylhexyl) phthalate (DEHP), bisphenol A (BPA) and irgarol. We characterized the M. japonicus VTG gene and analyzed the transcriptional expression of VTG mRNA in M. japonicus exposed to various chemicals and exposure periods. A phylogenetic analysis revealed that the M. japonicus VTG clustered closely with Eriocheir sinensis (Chinese mitten crab) VTG, in contrast with another clade that included the VTG ortholog of other crabs. The basal level of VTG expression was the highest in the hepatopancreas and ovaries, and tissues. VTG expression significantly increased in the ovaries and hepatopancreas after 24 h exposure to DEHP. Increased responses of VTG transcripts were found in M. japonicus exposed to DEHP and BPA for 96 h; however, VTG expression decreased in both tissues after irgarol exposure. After an exposure of 7 d, VTG expression significantly increased in the ovaries and hepatopancreas for all concentrations of all chemicals. These results suggest that the crustacean embryogenesis and endocrine processes are impaired by the environmental chemical pollutants DEHP, BPA, and irgarol.

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Characterization and gene expression of heat shock protein 90 in marine crab <i>Charybdis japonica</i> following bisphenol A and 4-nonylphenol exposures

Cited by 16 publications

References 39 publications

Identification of warm temperature acclimation‐associated 65‐kDa protein‐2 in Kumgang fat minnow Rhynchocypris kumgangensis

Identification of warm temperature acclimation‐associated 65‐kDa protein‐2 in Kumgang fat minnow Rhynchocypris kumgangensis

Genome reprogramming inSaccharomyces cerevisiaeupon nonylphenol exposure

Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

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