Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, <i>Janibacter</i> sp. strain OC11

Oba, Shintaro; Suzuki, Toshihiro; Maeda, Rintaro; Omori, Toshio; Fuse, Hiroyuki

doi:10.1080/09168451.2014.917260

Cited by 3 publications

(3 citation statements)

References 32 publications

(39 reference statements)

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“…According to previous studies, the genus Janibacter showed broad metabolic versatility toward a variety of aromatic compounds such as fluorene, carbazole (Oba et al, 2014;Yamazoe et al, 2004), anthracene, phenanthrene, dibenzofuran (Jin et al, 2006), and polychlorinated biphenyls (Sierra et al, 2003).…”

Section: Relationship Between Microdiversity Of Marine Actinobacteria...mentioning

confidence: 99%

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Ettoumi

Chouchane²,

Guesmi

et al. 2016

Microbiological Research

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In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n=49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n=22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.

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Section: Relationship Between Microdiversity Of Marine Actinobacteria...mentioning

confidence: 99%

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Ettoumi

Chouchane²,

Guesmi

et al. 2016

Microbiological Research

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“…Two tertiary structures have been reported for type I extradiol dioxygenases: homo-octamer for BphC 3) and homotetramer for catechol 2,3-dioxygenase. 5) For heterooligomeric extradiol dioxygenase type II enzymes, α 2 β 2 heterotetramers were reported for protocatechuate 4,5-dioxygenase (LigAB), 6) 2′-aminobiphenyl-2,3-diol 1,2-dioxygenase (CarBaBb), [21][22] and 2-aminophenol 1,6-dioxygenase (CnbCaCb). 20) The results obtained from the gel filtration chromatography suggested that FlnD1D2 has an α 4 β 4 heterooctameric structure, indicating that this enzyme has a novel, functionally stable tertiary structure in solution.…”

Section: Resultsmentioning

confidence: 99%

Purification and partial characterization of the extradiol dioxygenase, 2′-carboxy-2,3-dihydroxybiphenyl 1,2-dioxygenase, in the fluorene degradation pathway from Rhodococcus sp. strain DFA3

Kotake

Matsuzawa

Suzuki-Minakuchi

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Type II extradiol dioxygenase, 2'-carboxy-2,3-dihydroxybiphenyl 1,2-dioxygenase (FlnD1D2) involved in the fluorene degradation pathway of Rhodococcus sp. DFA3 was purified to homogeneity from a heterologously expressing Escherichia coli. Gel filtration chromatography and SDS-PAGE suggested that FlnD1D2 is an α4β4 heterooctamer and that the molecular masses of these subunits are 30 and 9.9 kDa, respectively. The optimum pH and temperature for enzyme activity were 8.0 and 30 °C, respectively. Assessment of metal ion effects suggested that exogenously supplied Fe(2+) increases enzyme activity 3.2-fold. FlnD1D2 catalyzed meta-cleavage of 2'-carboxy-2,3-dihydroxybiphenyl homologous compounds, but not single-ring catecholic compounds. The Km and kcat/Km values of FlnD1D2 for 2,3-dihidroxybiphenyl were 97.2 μM and 1.5 × 10(-2) μM(-1)sec(-1), and for 2,2',3-trihydroxybiphenyl, they were 168.0 μM and 0.5 × 10(-2) μM(-1)sec(-1), respectively. A phylogenetic tree of the large and small subunits of type II extradiol dioxygenases suggested that FlnD1D2 constitutes a novel subgroup among heterooligomeric type II extradiol dioxygenases.

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“…Janibacter sp. OC11 was later identified as the first marine Gram-positive carbazole degrader with a CARDO belonging to the class IIB like the one in strain IC177 (Oba et al 2014 ). Based on their diversity, known car gene clusters identified through heterologous expression and southern hybridization experiments were classified into three types: Pseudomonas -type ( car (P)), which shares homology with the car gene in P. resinovorans CA10; Sphingomonas -type car (SK), which shares homology with the car gene in Sphingomonas sp.…”

Section: Diversity Of Carbazole-degrading Bacteria Having the ...mentioning

confidence: 99%

The potential application of carbazole-degrading bacteria for dioxin bioremediation

Dinh,

Nguyen,

Nguyen

2023

Bioresour. Bioprocess.

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Extensive research has been conducted over the years on the bacterial degradation of dioxins and their related compounds including carbazole, because these chemicals are highly toxic and has been widely distributed in the environment. There is a pressing need to explore and develop more bacterial strains with unique catabolic features to effectively remediate dioxin-polluted sites. Carbazole has a chemical structure similar to dioxins, and the degradation pathways of these two chemicals are highly homologous. Some carbazole-degrading bacterial strains have been demonstrated to have the ability to degrade dioxins, such as Pseudomonas sp. strain CA10 và Sphingomonas sp. KA1. The introduction of strain KA1 into dioxin-contaminated model soil resulted in the degradation of 96% and 70% of 2-chlorodibenzo-p-dioxin (2-CDD) and 2,3-dichlorodibenzo-p-dioxin (2,3-DCDD), respectively, after 7-day incubation period. These degradation rates were similar to those achieved with strain CA10, which removed 96% of 2-CDD and 80% of 2,3-DCDD from the same model soil. Therefore, carbazole-degrading bacteria hold significant promise as potential candidates for dioxin bioremediation. This paper overviews the connection between the bacterial degradation of dioxins and carbazole, highlighting the potential for dioxin biodegradation by carbazole-degrading bacterial strains.

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Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, Janibacter sp. strain OC11

Cited by 3 publications

References 32 publications

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Purification and partial characterization of the extradiol dioxygenase, 2′-carboxy-2,3-dihydroxybiphenyl 1,2-dioxygenase, in the fluorene degradation pathway from Rhodococcus sp. strain DFA3

The potential application of carbazole-degrading bacteria for dioxin bioremediation

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