Characterization and Genomic Study of the Novel Bacteriophage HY01 Infecting Both Escherichia coli O157:H7 and Shigella flexneri: Potential as a Biocontrol Agent in Food

Lee, Heyn; Ku, Hye‐Jin; Lee, Dong‐Hoon; Kim, You‐Tae; Shin, Hakdong; Ryu, Sangryeol; Lee, Ju-Hoon

doi:10.1371/journal.pone.0168985

Cited by 69 publications

(62 citation statements)

References 33 publications

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“…Lee et al ( 2016 ) suggested that besides bacteriophage cocktails, a solution for simultaneous biocontrol of several foodborne pathogens could be the cloning of several receptor binding genes into a single bacteriophage. For either purpose, obtaining knowledge about new bacteriophages capable of infecting these pathogens and assessing their host spectrum is essential.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products

et al. 2018

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During recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenic Escherichia coli, Shigella, and Salmonella strains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples. All bacterophages effectively lysed E. coli K-12 strains and were able to infect pathogenic E. coli strains representing enterohaemorrhagic (EHEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes, Shigella dysenteriae, S. sonnei strains, as well as multidrug-resistant (MDR) E. coli and multiple strains representing different Salmonella enterica serovars. All the bacteriophages exhibited Siphoviridae morphology. Whole genome sequencing of the novel T5-like bacteriophages showed that they represent two distinct groups, with the genome-based grouping correlating to the different host spectra. As these bacteriophages are of food origin, their stability and lack of any virulence genes, as well as their broad and mutually complementary host spectrum makes these new T5-like bacteriophages valuable candidates for use as biocontrol agents against foodborne pathogenic enterobacteria.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products

et al. 2018

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“…It is also worth mentioning that the truncation of ORF1 annotated as "tail fibers" in P206 (ORF5 in C203) seems to have no effect on the host specificity of the phage when compared to C203. Lee et al (2016) have proposed that genes associated with host specificity should be cloned into one bacteriophage genome, which then could be used as a universal agent against several related pathogenic bacteria. Broad host spectrum bacteriophages such as C203/P206 could be a potential source of such host specificity associated genes, or alternatively, it could be used in its native form as biocontrol agent either as a stand-alone or in phage cocktails.…”

Section: Discussionmentioning

confidence: 99%

“…One step growth experiment to determine burst size was performed on E. coli MG1655 according the protocol of Lee et al, (2016) with some modifications. Briefly, 2x10 8 bacteria were mixed with 2x10 6 phages, setting multiplicity of infection (MOI) to 0.01 in Luria-Bertani broth (LB), and incubated for 20 minutes at room temperature.…”

Section: One Step Growth Experimentsmentioning

confidence: 99%

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Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Sváb

Falgenhauer

Rohde

et al. 2018

Infection, Genetics and Evolution

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We isolated and characterized two novel rV5-like lytic bacteriophages from independently collected food samples. Nucleotide sequence analysis revealed that these phages have linear double-stranded DNA genomes comprising 138,073 bp with 213 CDS and 5 tRNA genes. The two genomes contain completely identical nucleotide sequence, albeit there is a 10,718 bp-long shift in the sequence. The GC content of the phage genomes was 43.7% and they showed high general homology to rV5-like phages. The new phages were termed C203 and P206. The genome of both phages contains a unique ORF that encodes for a putative phage homing endonuclease. The phage produced clear plaques with a burst size of approx. 1000 viral particles and a latent period of 60 min. Morphological investigation indicated that the new phages are members of the family Myoviridae with an approximate head length of 85 nm, tail length of 75 nm, and a head width of 96 nm. C203 and P206 exhibit a broad and uniform host range, which included enterohemorrhagic Escherichia coli strains of serogroup O157, multi drug resistant (MDR) E. coli strains of various sero- and pathotypes, and both Shigella sonnei and S. dysenteriae strains. C203 and P206 both effectively reduced the number of living EHEC O157:H7 Sakai in experimentally inoculated minced meat. The same broad host range, the lack of any virulence related genes, the stability and its short latent period suggest that these newly found phages could be suitable candidates as a bio-control agents against food-borne pathogenic Enterobacteria.

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“…In this study, a new lytic phage vB_ShiP-A7 was isolated and characterized, with lytic activity against both MDR S. exneri clinical strain and several MDR E. coli clinical strains (Table 1). Several phages were reported to have the ability to infect both E. coli and Shigella [40,41,42]. Since E. coli and Shigella are genetically similar, it is reasonable to expect some similar phages would target both strains.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Genomic Analysis of Novel Phage vB_ ShiP-A7 Infecting Multidrug-Resistant Shiglla Fexneri and Escherichia Coli

Xu¹,

Gu²,

Yu³

et al. 2020

Preprint

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BackgroundPhage therapy has regained more attention due to the rise of multidrug-resistant (MDR) bacteria. Several case reports demonstrated clinical application of phage in resolving infections caused by MDR bacteria in recent years. ResultsWe isolated a new phage, vB_ShiP-A7, and then investigated its characteristics. Phage vB_ShiP-A7 is a member of Podoviridae that has an icosahedral spherical head and a short tail. vB_ShiP-A7 has large burst size and short replication time. vB_ShiP-A7’s genome is linear double stranded DNA composed of 40058 bp, encoding forty-three putative open reading frames. Comparative genome analysis demonstrated vB_ShiP-A7’s genome sequence is closely related to fifteen different phages (coverage 74-88%, identity 86-93%). Mass Spectrometry analysis revealed that twelve known proteins and six hypothetical proteins exist in particles of vB_ShiP-A7. Genome and proteome analyses confirmed the absence of lysogen-related proteins and toxic proteins in this phage. In addition, phage vB_ShiP-A7 can significantly reduce the growth of clinical MDR stains of Shigella ﬂexneri and Escherichia coli in liquid culture. Furthermore, vB_ShiP-A7 can disrupt biofilms formed by Shigella ﬂexneri or Escherichia coli in vitro. ConclusionPhage vB_ShiP-A7 is a stable novel phage, which has a strong application potential to inhibit MDR stains of Shigella ﬂexneri and Escherichia coli. Comparing the genomes between vB_ShiP-A7 and other closely-related phages will help us better understand the evolutionary mechanism of phages.

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Characterization and Genomic Study of the Novel Bacteriophage HY01 Infecting Both Escherichia coli O157:H7 and Shigella flexneri: Potential as a Biocontrol Agent in Food

Cited by 69 publications

References 33 publications

Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products

Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products

Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Molecular Characterization and Genomic Analysis of Novel Phage vB_ ShiP-A7 Infecting Multidrug-Resistant Shiglla Fexneri and Escherichia Coli

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