Characterization and Identification of Phytate Solubilizing Yeasts Isolated from Food Grains

Suvarna, P.D. Savita V.C.; Balakrishna, T. Annu A.N.; Yallappa, B. Kanchanashri M.

doi:10.20546/ijcmas.2017.604.145

Cited by 5 publications

(2 citation statements)

References 17 publications

(11 reference statements)

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“…Phytate (myo-inositol 1, 2, 3, 4, 5, 6hexakisphosphate, IP6) is an abundant plant constituent (Gerke 2015; Cowieson et al 2017;Savita et al 2017) which comprises of 1-5% of the total weight of nuts, cereals, oilseeds, pollen, and other legumes. Phytate is the main storage form of inositol and phosphorus (P) in the plant seed (Naves et al 2012;Chen et al 2015).…”

Section: Introductionmentioning

confidence: 99%

BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM <em>Klebsiella</em> sp. ASR1 ENCAPSULATED WITH ALGINATE

Hidayatullah

Sajidan

Susilowati

et al. 2020

J Bioteknol Biosains Indones

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Karakterisasi Biokimia Enzim Fitase Rekombinan (phyK) dari Klebsiella sp. ASR1 Yang Dienkapsulasi Dengan AlginatEnzim fitase melepas molekul fosfor pada atom C dari benzena Inositol fitat. Tetapi fitase memiliki kelemahan tidak mampu bertahan terhadap kondisi ekstrim dalam lambung nonruminansia. Solusi dalam penelitian ini yaitu fitase dienkapsulasi menggunakan alginat. Penelitian ini bertujuan mengkarakterisasi fitase setelah dienkapsulasi menggunakan alginate. Hasil penelitian ini yaitu fitase yang dienkapsulasi memiliki aktivitas tertinggi pada pH 6,0, sedangkan fitase tanpa enkapsulasi pada pH 5,0. Suhu optimum untuk aktivitas tertinggi fitase yang dienkapsulasi yaitu 70ÂºC, sedangkan fitase tanpa enkapsulasi 37ÂºC. Untuk perlakuan penambahan ion logam, aktivitas tertinggi fitase yang dienkapsulasi terjadi dengan penambahan 0,1 mM Fe2+ dan 1,0 mM Ca2+, sedangkan fitase tanpa enkapsulasi dengan penambahan 0,1 mM Fe2+. Berdasarkan hasil penelitian ini, fitase yang dienkapsulasi memiliki keunggulan lebih banyak dibandingkan dengan fitase tanpa enkapsulasi, karena mampu bertahan pada pH dan suhu tinggi, dan beberapa efek ion logam.Kata Kunci: alginat, asam fitat, enkapsulasi, fitase, fitase rekombinanABSTRACTPhytase enzymes release phosphorus molecules on the C atom from benzene inositol phytate. But phytase has the disadvantage of being unable to withstand extreme conditions in the non-ruminant stomach. The solution in this research was phytase encapsulated using alginate. This study aims to characterize phytase after being encapsulated using alginate. The results of this study were the encapsulated phytase had the highest activity at pH 6.0, while the unencapsulated phytase at pH 5.0. The optimum temperature for the highest activity of the encapsulated phytase was 70ÂºC, while the unencapsulated phytase 37ÂºC. For treatment of metal ion addition, the highest activity of the encapsulated phytase occurred with the addition of 0.1 mM Fe2+ and 1.0 mM Ca2+, while the unencapsulated phytase with the addition of 0.1 mM Fe2+. Based on the results of this study, the encapsulated phytase had more advantages compared to the unencapsulated phytase, as the former withstand high pH and temperature, and some metal ion effects.

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Section: Introductionmentioning

confidence: 99%

BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM <em>Klebsiella</em> sp. ASR1 ENCAPSULATED WITH ALGINATE

Hidayatullah

Sajidan

Susilowati

et al. 2020

J Bioteknol Biosains Indones

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“…Yeasts are rich source of proteins, vitamins, and P (Querol and Fleet 2006), and hence, the use of cell-bound phytaseproducing yeast as a feed/food additive may be expected to improve its nutritional value when compared with the use of conventional free phytase. Several yeast strains have been screened for their cell-bound and extracellular phytase production ability (Menezes et al 2019;Savita et al 2017;Nuobariene et al 2011); however, because of low phytase productivities, very few of them have actually made it to the application level. Recently, Chen (2017) stated that phytase displayed on yeast surface can provide a beneficial novel platform for developing an environment friendly system for its industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Use of Plackett–Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production

et al. 2019

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Distiller-dried grain solid (DDGS), a co-product of alcohol production, contains cereal grain residues, proteins, and yeast metabolites, which make it suitable in poultry feeding. However, high phytate content of DDGS limits its applicability in poultry feed. In this study, Plackett-Burman design was used to improve cell-bound phytase production by Williopsis saturnus NCIM 3298, and we achieved an enzyme activity of 269 IU/g of dry-wet biomass. The effect of this enhanced phytase-displaying yeast strain on hydrolysis of corn phytate and subsequently on ethanol production and DDGS quality was then investigated. Results of saccharification in the presence of phytase showed that reducing sugar content of liquefied mash increased by 11%, which subsequently improved the ethanol production by 18% (w/v) (p < 0.01) compared with the control. Notably, phytase treatment decreased the phytate content of corn by 70% (p < 0.01) compared with the control, thereby improving the availability of free phosphate in fermentation broth and DDGS. Thus, the results obtained suggest that the addition of W. saturnus NCIM 3298 strain has the potential of providing a new source of phytase that would be useful in the feed and ethanol industries.

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Evaluation of Candida tropicalis (NCIM 3321) extracellular phytase having plant growth promoting potential and process development

Puppala

Naik

Shaik

et al. 2018

Biocatalysis and Agricultural Biotechnology

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Characterization and Identification of Phytate Solubilizing Yeasts Isolated from Food Grains

Cited by 5 publications

References 17 publications

BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM <em>Klebsiella</em> sp. ASR1 ENCAPSULATED WITH ALGINATE

BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM <em>Klebsiella</em> sp. ASR1 ENCAPSULATED WITH ALGINATE

Use of Plackett–Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production

Evaluation of Candida tropicalis (NCIM 3321) extracellular phytase having plant growth promoting potential and process development

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