Characterization and interstrain transfer of prophage pp3 of Pseudomonas aeruginosa

Li, Gang; Lu, Shuguang; Shen, Mengyu; Le, Shuai; Shen, Wei; Tan, Yinling; Wang, Jing; Zhao, Xia; Zhao, Yan; Gong, Yanhong; Yang, Yuhui; Zhu, Huajian; Hu, Fuquan; Li, Ming

doi:10.1371/journal.pone.0174429

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…In recent years, phage therapy has been shown as a promising alternative to conventional antibiotic therapy ( Kortright et al., 2019 ). We previously found that several new lytic phages show effective antimicrobial potential against A. baumannii ( Yang et al., 2019 ), P. aeruginosa ( Li et al., 2017 ), and K. pneumoniae ( Shi et al., 2020 ). However, the clinical application of a 12-phage cocktail on P. aeruginosa infected burn wounds showed poor results compared with 1% sulfadiazine silver ( Jault et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards

Gong

Peng

Luo

et al. 2021

Front. Cell. Infect. Microbiol.

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Infection is the leading cause of complications and deaths after burns. However, the difference in infection patterns between the burn intensive care unit (BICU) and burn common wards (BCW) have not been clearly investigated. The present study aimed to compare the infection profile, antimicrobial resistance, and their changing patterns in burn patients in BICU and BCW. Clinical samples were analyzed between January 1, 2011, and December 31, 2019, in the Institute of Burn Research in Southwest China. The patient information, pathogen distribution, sources, and antimicrobial resistance were retrospectively collected. A total of 3457 and 4219 strains were detected in BICU and BCW, respectively. Wound secretions accounted for 86.6% and 44.9% in BCW and BICU, respectively. Compared with samples in BCW, samples in BICU had more fungi (11.8% vs. 8.1%), more Gram-negative bacteria (60.0% vs. 50.8%), and less Gram-positive bacteria (28.2% vs. 41.1%). Acinetobacter baumannii were the most common pathogen in BICU, compared with Staphylococcus aureus in BCW. S. aureus was the most frequent pathogen in wound secretions and tissues from both BICU and BCW. However, A. baumannii were the first in blood, sputum, and catheter samples from BICU. Overall, the multidrug-resistance (MDR) rate was higher in BICU than in BCW. However, the gap between BICU and BCW gradually shortened from 2011 to 2019. The prevalence of MDR A. baumannii and Klebsiella pneumonia significantly increased, especially in BCW. Furthermore, Carbapenem resistance among K. pneumoniae significantly increased in BICU (4.5% in 2011 vs. 40% in 2019) and BCW (0 in 2011 vs. 40% in 2019). However, the percentage of MDR P. aeruginosa sharply dropped from 85.7% to 24.5% in BICU. The incidence of MRSA was significantly higher in BICU than in BCW (94.2% vs. 71.0%) and stayed at a high level in BICU (89.5% to 96.3%). C. tropicalis and C. albicans were the two most frequent fungi. No resistance to Amphotericin B was detected. Our study shows that the infection profile is different between BICU and BCW, and multidrug resistance is more serious in BICU than BCW. Therefore, different infection-control strategies should be emphasized in different burn populations.

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Section: Discussionmentioning

confidence: 99%

Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards

Gong

Peng

Luo

et al. 2021

Front. Cell. Infect. Microbiol.

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“…The obtained PCR fragment was cloned into the KpnI/PstI sites of plasmid pUCP24 ( West et al, 1994 ), resulting in pUCP wzy . Construct pUCP wzy was then electroporated into PA1RG to obtain the complementary strain PA1RG/ wzy as described previously ( Li et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Adaptation of Pseudomonas aeruginosa to Phage PaP1 Predation via O-Antigen Polymerase Mutation

Shen

Yang

et al. 2018

Front. Microbiol.

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Adaptation of bacteria to phage predation poses a major obstacle for phage therapy. Bacteria adopt multiple mechanisms, such as inhibition of phage adsorption and CRISPR/Cas systems, to resist phage infection. Here, a phage-resistant mutant of Pseudomonas aeruginosa strain PA1 under the infection of lytic phage PaP1 was selected for further study. The PaP1-resistant variant, termed PA1RG, showed decreased adsorption to PaP1 and was devoid of long chain O-antigen on its cell envelope. Whole genome sequencing and comparative analysis revealed a single nucleotide mutation in the gene PA1S_08510, which encodes the O-antigen polymerase Wzy that is involved in lipopolysaccharide (LPS) biosynthesis. PA1_Wzy was classified into the O6 serotype based on sequence homology analysis and adopts a transmembrane topology similar to that seem with P. aeruginosa strain PAO1. Complementation of gene wzy in trans enabled the mutant PA1RG to produce the normal LPS pattern with long chain O-antigen and restored the susceptibility of PA1RG to phage PaP1 infection. While wzy mutation did not affect bacterial growth, mutant PA1RG exhibited decreased biofilm production, suggesting a fitness cost of PA1 associated with resistance of phage PaP1 predation. This study uncovered the mechanism responsible for PA1RG resistance to phage PaP1 via wzy mutation and revealed the role of phages in regulating bacterial behavior.

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“…Several groups proposed and applied AMPs and other novel agents to treat and control the infection of P. aeruginosa and other similar pathogens [25,37,38]. More attention should be paid on the relationship between the bactericidal effects, quorum sensing system, and T3SS and their functions in bacterial infection.…”

Section: Discussionmentioning

confidence: 99%

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14

Liu

Dong

et al. 2020

Braz J Microbiol

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Antimicrobial peptides are considered to be one of the candidate antimicrobial agents for antibiotic-resistant bacterial infection in the future. The effects of antimicrobial peptide hBD3-CBD on Pseudomonas aeruginosa PA14 and PA14 ΔexsA were analyzed by the bactericidal effects, hemolysis assays, pyocyanin pigment productions, and virulence factor expressions (exoU, exoS, hcnA, and lasB). Pyocyanin production and virulence factor expressions are important features of the type III secretion system in Pseudomonas aeruginosa. HBD3-CBD killed PA14 and PA14 ΔexsA with similar efficiency; it lowered the hemolysis levels of PA14 and PA14 ΔexsA and reduced the pyocyanin production, biofilm formation, and exoU, exoS, and lasB expressions in PA14. Compared with PA14, PA14 ΔexsA showed a lower hemolysis effect, pyocyanin production, exoU, and lasB expressions. The effects of hBD3-CBD on the PA14 toxin secretion were similar to the changes in the type III secretion system mutant isolate PA14 ΔexsA. Our results demonstrated that the type III secretion system was involved in the biological functions on PA 14 from hBD3-CBD. Keywords Antimicrobial peptide. hBD3. Pseudomonas aeruginosa. T3SS Antimicrobial peptides (AMPs) are polypeptides with broadspectrum antimicrobial activity against bacteria, fungi, and other microorganisms [1]. Their potent activity at low concentrations against many pathogenic organisms, including multiresistant strains, makes AMPs attractive candidates as antimicrobial agents to many inflammatory and infectious diseases [2]. Human beta-defensin-3 (hBD3) is a multifunctional peptide and widely expressed in oral and other tissues. The antiinfective effects of this polypeptide have been confirmed by several studies [3-5].

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Characterization and interstrain transfer of prophage pp3 of Pseudomonas aeruginosa

Cited by 6 publications

References 45 publications

Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards

Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards

Adaptation of Pseudomonas aeruginosa to Phage PaP1 Predation via O-Antigen Polymerase Mutation

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14

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