Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

Abstract

Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate (EMS). F 2 and F 2:3 generations produced from crosses of M8008 × YN15 (MY) and M8008 × SJZ54 (MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height (PH), spike length (SL), fertile spikelet number per spike (FSS), grain width (GW), grain length (GL), GL/GW ratio (GLW), and thousand-grain weight (TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat (SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci (QTLs) forming QTL clusters (one in MY and two in MS) were detected. The QTL clusters coinciding with (MY population) or near (MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density (FFD), and TGW and to increases in GLW. The cluster in the wmc25-barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant (SN), and SL.

Association between Grain Size and Shape and Quality Traits, and Path Analysis of Thousand Grain Weight in Iranian Bread Wheat Landraces from Different Geographic Regions

“…233 al., 2013). Only few studies are available based on digital image analysis of grain size and shape in bread wheat (Williams et al, 2013;Gegas et al, 2010;Williams and Sorrells, 2014;Breseghello and Sorrells, 2007;Xiao et al, 2011;Rasheed et al, 2014;Zhang et al, 2015). Among these studies, Gegas et al, 2010;Williams et al (2013), Williams and Sorrells (2014) and Rasheed et al (2014), used shape variations as targeted traits influencing grain size and weight and results are comparable to our work.…”

“…The high significant differences (P < 0.001) among genotypes for all traits indicating the presence of adequate variability among the bread wheat local landraces which can be used for the improvement of grain weight and quality in order to create high yielding varieties with good end-use quality. Though compare the measures for traits such as grain size and shape in different studies because of different approaches to converting raw images into quantitative data is not completely correct, differences between high and low level for the most traits in this study are more in compared with other studies such as Sun et al (2009),Tsilo et al (2010, Jia et al (2013) and Zhang et al (2015). Due to the high diversity, it seems can find suitable genotypes with high and low limit bounds for two or more traits as versus each other.…”

QTL Mapping of Kernel Traits and Validation of a Major QTL for Kernel Length-Width Ratio Using SNP and Bulked Segregant Analysis in Wheat

“…In addition, there are many reports of QTLs for TKW on chromosome 1B 1,7,27,28 . However, previous studies have found QTLs for TKW on almost all chromosomes of the wheat genome 1,2,[6][7][8][10][11][12][13]25,[27][28][29][30][31][32][33][34][35] . Kernel-related traits are susceptible to environmental impact, and the observed values of the same plant materials in different environments were also significantly different.…”

Genetic Variation Analysis of EMS-Induced Chili Pepper (Capsicum frutescens L.) Mutants Using SSR Markers

“…Genomic changes in plant induced mutation with EMS can be caused by mutagenesis mechanism which a G/C to A/T nucleotides change in the primer binding regions of SSR marker [23,24,25]. In addition, insertion or deletion of nucleotide in the DNA sequences of mutant plants inducing the lengthening or shortening repeat region of microsatellite marker [24,26,27].…”