Characterization and mechanism of Mn(II)-based mixotrophic denitrifying bacterium (Cupriavidus sp. HY129) in remediation of nitrate (NO3−-N) and manganese (Mn(II)) contaminated groundwater

Bai, Yihan; Su, Junfeng; Qin, Wen; Huang, Tinglin; Chang, Qiao; Ali, Amjad

doi:10.1016/j.jhazmat.2020.124414

Cited by 67 publications

(13 citation statements)

References 53 publications

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“…(Su et al, 2017; and Cupriavidus sp. (Bai et al, 2020), both of which were isolated from the sediments of one reservoir in Xi'an China, were capable of performing simultaneous denitrification and removal of Cd(II) and Mn(II) (Pseudomonas sp. ), and simultaneous removal of nitrate and Mn(II) (Cupriavidus sp.)…”

Section: Mixotrophic Bacteria Containing No Photosynthetic Pigmentsmentioning

confidence: 99%

“…These bacteria could be potentially used as an alternative to conventional flotation reagents, or for the treatment of NO3 --N, Cd(II) and Mn(II) co-contaminated groundwater. Organic matters in the environment and secreted by these mixotrophic bacteria (e.g., EPS) have been speculatively contributed to the removal of these contaminants (Bai et al, 2020;Chaerun et al, 2017;. Considering the formation of Fe(II)-organic complexes, and their subsequent inhibition of Fe(II) oxidation via mixotrophic nitrate-reducing Fe(II)-oxidizing bacterium (e.g., Acidovorax sp.)…”

Section: Mixotrophic Bacteria Containing No Photosynthetic Pigmentsmentioning

confidence: 99%

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Mixotrophic bacteria for environmental detoxification of contaminated waste and wastewater

Huang

Xing

Zhou

et al. 2021

Appl Microbiol Biotechnol

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Mixotrophic bacteria provide a desirable alternative to the use of classical heterotrophic or chemolithoautotrophic bacteria in environmental technology, particularly under limiting nutrients conditions. Their bi-modal ability of adapting to inorganic or organic carbon feed and sulfur, nitrogen or even heavy metals stress conditions are attractive features to achieve efficient bacterial activity and favorable operation conditions for the environmental detoxification or remediation of contaminated waste and wastewater. This review provides an overview on the state of the art and summarizes the metabolic traits of the most promising and emerging non-model mixotrophic bacteria for the environmental detoxification of contaminated wastewater and waste containing excess amounts of limiting nutrients. Although mixotrophic bacteria usually function with low organic carbon sources, the unusual capabilities of mixotrophic electroactive exoelectrogens and electrotrophs in bioelectrochemical systems and in microbial electrosynthesis for accelerating simultaneous metabolism of inorganic or organic C and N, S or heavy metals are reviewed. The identification of the mixotrophic properties of electroactive bacteria and their capability to drive mono or bidirectional electron transfer processes are highly exciting and promising aspects. These aspects provide an appealing potential for unearthing new mixotrophic exoelectrogens and electrotrophs, and thus inspire next generation of microbial electrochemical technology and mixotrophic bacterial metabolic engineering.

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Section: Mixotrophic Bacteria Containing No Photosynthetic Pigmentsmentioning

confidence: 99%

Section: Mixotrophic Bacteria Containing No Photosynthetic Pigmentsmentioning

confidence: 99%

Mixotrophic bacteria for environmental detoxification of contaminated waste and wastewater

Huang

Xing

Zhou

et al. 2021

Appl Microbiol Biotechnol

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“…Some genera, such as Rheinheimera (PMW) and Ralstonia (PM11 and PMW), are known to be involved in the iron cycle ( Swanner et al, 2011 ; Schröder et al, 2016 ), while Aphanizomenon NIES81 (PMW), Herbaspirillum, Methylocella , and Ralstonia (PM11 and PMW) are involved in the N cycle ( Ploug et al, 2010 ; Dalsing et al, 2015 ; Dedysh and Dunfield, 2016 ; Waller et al, 2021 ). Bacteria involved in the Mn cycle ( Yang et al, 2013 ; Bai et al, 2021 ; Lee et al, 2021 ) were identified as Escherichia/Shigella, Halomonas, Microbacterium (PMW), and Cupriavidus (PMW and PMB). Elements, such as N, Fe, and P, are usually found in guano ( Miko et al, 2001 ; Wurster et al, 2015 ; Misra et al, 2019 ), P is also associated with bones ( Audra et al, 2019 ), and Mn deposits are usually biogenic in caves ( Northup and Lavoie, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

A 16S rRNA Gene-Based Metabarcoding of Phosphate-Rich Deposits in Muierilor Cave, South-Western Carpathians

et al. 2022

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Muierilor Cave is one of Romania’s most important show caves, with paleontological and archeological deposits. Recently, a new chamber was discovered in the cave, with unique yellow calcite crystals, fine-grained crusts, and black sediments. The deposits in this chamber were related to a leaking process from the upper level that contains fossil bones and a large pile of guano. Samples were taken from the new chamber and another passage to investigate the relationship between the substrate and microbial community. Chemical, mineralogical, and whole community 16S rRNA gene-based metabarcoding analyses were undertaken, and the base of the guano deposit was radiocarbon dated. Our study indicated bacteria linked to the presence of high phosphate concentration, most likely due to the nature of the substrate (hydroxyapatite). Bacteria involved in Fe, Mn, or N cycles were also found, as these elements are commonly identified in high concentrations in guano. Since no bat colonies or fossil bones were present in the new chamber, a high concentration of these elements could be sourced by organic deposits inside the cave (guano and fossil bones) even after hundreds of years of their deposition and in areas far from both deposits. Metabarcoding of the analyzed samples found that ∼0.7% of the identified bacteria are unknown to science, and ∼47% were not previously reported in caves or guano. Moreover, most of the identified human-related bacteria were not reported in caves or guano before, and some are known for their pathogenic potential. Therefore, continuous monitoring of air and floor microbiology should be considered in show caves with organic deposits containing bacteria that can threaten human health. The high number of unidentified taxa in a small sector of Muierilor Cave indicates the limited knowledge of the bacterial diversity in caves that can have potential applications in human health and biotechnology.

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“…Bacterial MCOs are recognized as significant contributors to the Mn(II) oxidation process. Bacteria may have developed many strategies to adapt to environments containing extremely toxic quantities of manganese (Bai et al, 2021), and Mn(II) oxidation is a crucial mechanism used by MOB to facilitate survival in high Mn surroundings. The presence of Mn oxides outside of the bacteria provide protection against ultraviolet (UV) ionizing radiation, viral attack, and heavy metal toxicity (Daly et al, 2004;Ghosal et al, 2005).…”

Section: Differentially Expressed Genes Under Manganese Stressmentioning

confidence: 99%

Manganese Stress Adaptation Mechanisms of Bacillus safensis Strain ST7 From Mine Soil

et al. 2021

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The mechanism of bacterial adaption to manganese-polluted environments was explored using 50 manganese-tolerant strains of bacteria isolated from soil of the largest manganese mine in China. Efficiency of manganese removal by the isolated strains was investigated using atomic absorption spectrophotometry. Bacillus safensis strain ST7 was the most effective manganese-oxidizing bacteria among the tested isolates, achieving up to 82% removal at a Mn(II) concentration of 2,200 mg/L. Bacteria-mediated manganese oxide precipitates and high motility were observed, and the growth of strain ST7 was inhibited while its biofilm formation was promoted by the presence of Mn(II). In addition, strain ST7 could grow in the presence of high concentrations of Al(III), Cr(VI), and Fe(III). Genome-wide analysis of the gene expression profile of strain ST7 using the RNA-seq method revealed that 2,580 genes were differently expressed under Mn(II) exposure, and there were more downregulated genes (n = 2,021) than upregulated genes (n = 559) induced by Mn stress. KAAS analysis indicated that these differently expressed genes were mainly enriched in material metabolisms, cellular processes, organism systems, and genetic and environmental information processing pathways. A total of twenty-six genes from the transcriptome of strain ST7 were involved in lignocellulosic degradation. Furthermore, after 15 genes were knocked out by homologous recombination technology, it was observed that the transporters, multicopper oxidase, and proteins involved in sporulation and flagellogenesis contributed to the removal of Mn(II) in strain ST7. In summary, B. safensis ST7 adapted to Mn exposure by changing its metabolism, upregulating cation transporters, inhibiting sporulation and flagellogenesis, and activating an alternative stress-related sigB pathway. This bacterial strain could potentially be used to restore soil polluted by multiple heavy metals and is a candidate to support the consolidated bioprocessing community.

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Characterization and mechanism of Mn(II)-based mixotrophic denitrifying bacterium (Cupriavidus sp. HY129) in remediation of nitrate (NO3−-N) and manganese (Mn(II)) contaminated groundwater

Cited by 67 publications

References 53 publications

Mixotrophic bacteria for environmental detoxification of contaminated waste and wastewater

Mixotrophic bacteria for environmental detoxification of contaminated waste and wastewater

A 16S rRNA Gene-Based Metabarcoding of Phosphate-Rich Deposits in Muierilor Cave, South-Western Carpathians

Manganese Stress Adaptation Mechanisms of Bacillus safensis Strain ST7 From Mine Soil

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