Characterization and molecular evaluation of Staphylococcus aureus isolated from poultry and dairy cattle milk in Iraq

Almousawi, Anas E.; Alhatami, Abdullah O.; Neama, Noor A.; Baqir, Aiman Mohammed

doi:10.1063/5.0066975

Cited by 4 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research addressing the issue of antibacterial resistant bacteria in Iraq includes many works in the recent years. Most researchers focused on bacterial species such as: Salmonella species [36][37][38][39][40][41][42], Campylobacter species [43][44][45], Staphylococcus aureus [46,47] and Escherichia coli [48] and [49].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Ibrahim¹,

Jaafer²,

Fayyadh³

2022

PEN

View full text Add to dashboard Cite

Antibacterial resistant bacteria cause a big concern to poultry and to public health in general because some bacterial poultry pathogens can infect humans or transfer their resistance ability to human pathogens. Nontherapeutic use of antibacterial in poultry, especially as growth promoters to increase feed conversion efficiency is thought to be one of the main causes of resistance. The study included pulmonary swab samples collected during necropsy from 120 poultry farms showing respiratory symptoms with mortality. The disc diffusion method for antibiotic sensitivity testing was performed and antibiotic disks for 21 antibiotics were used. The results showed that three antibacterial were sensitive to more than 50% of the isolates. The first is doxycycline and 69.9% of the isolates were sensitive. The second is Cefalexin with 60.5% sensitive isolates and third is Chloramphenicol with 55.2% sensitive isolates. In the rest of antibacterial, less than 50% were sensitive. Five isolates were found resistant to all antibacterial. Moreover, three samples were found to be negative with no bacterial growth. The present study concluded that 50% of the aerobic bacteria isolated from poultry lungs are resistant to 85% of the 21 antibiotics tested in the study.

show abstract

Section: Resultsmentioning

confidence: 99%

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Ibrahim¹,

Jaafer²,

Fayyadh³

2022

PEN

View full text Add to dashboard Cite

show abstract

“…The most common symptoms of Campylobacter infections are diarrhea, fever, vomiting, and stomach cramps [14,15]. S. aureus is normally found in birds, meat and milk [16]. S. aureus is one of the common bacteria that display antimicrobial resistance, to antibiotics like methicillin and vancomycin [17,18].…”

Section: Introductionmentioning

confidence: 99%

Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective

Zhao,

Bhat,

O’Connor

et al. 2024

Preprint

View full text Add to dashboard Cite

Foodborne illnesses can be infectious and dangerous and most of them are caused by bacteria. Some types of common food-related bacteria exist widely in nature and pose a serious threat to both humans and animals, and can cause poisoning, diseases, disabilities and even death. Rapid, reliable and cost-effective methods for bacteria detection are of paramount importance in food-safety and environmental monitoring. Polymerase chain reaction (PCR), lateral flow immunochromatographic assay (LFIA) and electrochemical method have been widely used in food-safety and environmental monitoring. In this paper, the recent developments (2013-2023) covering PCR, LFIA and electrochemical method for various bacteria detection (Salmonella, Listeria, Campylobacter, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)) considering different foods types, analytical performances and the reported limit of detection (LOD) are discussed. It is found that the bacteria type and food sample type contribute significantly to the analytical performance and LOD. Detection by LFIA has higher average LOD (24 CFU/ml) than detection by electrochemical method (12 CFU/ml) and PCR (6 CFU/ml). Salmonella and E. coli in the Pseudomonadota domain usually have low LODs. LODs are usually lower for detection in fish and eggs. LFIA with gold and iron nanoparticles are prominent in the majority of articles of 26 CFU/ml and 12 CFU/ml respectively. Electrochemical method reveals that the average LOD is highest for cyclic voltammetry (CV) of 18 CFU/ml), followed by electrochemical impedance spectroscopy (EIS) of 12 CFU/ml and differential pulse voltammetry (DPV) of 8 CFU/ml. LOD usually decreases when replicate number increases until it remain unchanged. Exponential relations with (R2>0.95) between LOD of Listeria in milk by LFIA and electrochemical method with replicate number have been obtained. Finally, the review discusses the challenges and future perspectives (including the role of nanomaterials/advanced materials) to improve the analytical performance for bacterial detection.

show abstract

“…The most common symptoms of Campylobacter infections are diarrhea, fever, vomiting, and stomach cramps [14][15]. S. aureus is normally found in birds, meat and milk [16]. S. aureus is one of the common bacteria that display antimicrobial resistance, to antibiotics like methicillin and vancomycin [17][18].…”

Section: Introductionmentioning

confidence: 99%

Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective

Zhao,

Bhat,

O’Connor

et al. 2024

Preprint

View full text Add to dashboard Cite

Foodborne illnesses can be infectious and dangerous and most of them are caused by bacteria. Some types of common food-related bacteria exist widely in nature and pose a serious threat to both humans and animals, and can cause poisoning, diseases, disabilities and even death. Rapid, reliable and cost-effective methods for bacteria detection are of paramount importance in food-safety and environmental monitoring. Polymerase chain reaction (PCR), lateral flow immunochromatographic assay (LFIA) and electrochemical methods have been widely used in food-safety and environmental monitoring. In this paper, the recent developments (2013-2023) covering PCR, LFIA and electrochemical methods for various bacteria detection (Salmonella, Listeria, Campylobacter, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)) considering different foods types, analytical performances and the reported limit of detection (LOD) are discussed. It is found that the bacteria type and food sample type contribute significantly to the analytical per-formance and LOD. Detection by LFIA has higher average LOD (24 CFU/ml) than detection by electrochemical methods (12 CFU/ml) and PCR (6 CFU/ml). Salmonella and E. coli in the Pseudomonadota domain usually have low LODs. LODs are usually lower for detection in fish and eggs. LFIA with gold and iron nanoparticles are prominent in the majority of articles of 26 CFU/ml and 12 CFU/ml respectively. Electrochemical methods reveal that the average LOD is highest for cyclic voltammetry (CV) of 18 CFU/ml), followed by electrochemical impedance spectroscopy (EIS) of 12 CFU/ml and differential pulse voltammetry (DPV) of 8 CFU/ml. Finally, the review discusses the challenges and future perspectives (including the role of nanomaterials/advanced materials) to improve the analytical performance for bacterial detection.

show abstract

Characterization and molecular evaluation of Staphylococcus aureus isolated from poultry and dairy cattle milk in Iraq

Cited by 4 publications

References 49 publications

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective

Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective

Contact Info

Product

Resources

About