Characterization and Potential Use of Cuttlefish Skin Gelatin Hydrolysates Prepared by Different Microbial Proteases

Abstract

Composition, functional properties, and in vitro antioxidant activities of gelatin hydrolysates prepared from cuttlefish skin were investigated. Cuttlefish skin gelatin hydrolysates (CSGHs) were obtained by treatment with crude enzyme preparations from Bacillus licheniformis NH1, Bacillus mojavensis A21, Bacillus subtilis A26, and commercial alcalase. All CSGHs had high protein contents, 74.3–78.3%, and showed excellent solubility (over 90%). CSGH obtained by alcalase demonstrated high antioxidant activities monitored by β-carotene bleaching, DPPH radical scavenging, lipid peroxidation inhibition, and reducing power activity. Its antioxidant activity remained stable or increased in a wide range of pH (1–9), during heating treatment (100°C for 240 min) and after gastrointestinal digestion simulation. In addition, alcalase-CSGH was incorporated into turkey meat sausage to determine its effect on lipid oxidation during 35 days of storage period. At 0.5 mg/g, alcalase-CSGH delayed lipid oxidation monitored by TBARS and conjugated diene up to 10 days compared to vitamin C. The results reveal that CSGHs could be used as food additives possessing both antioxidant activity and functional properties.

Hydrolysed collagen from Lates calcarifer skin: its acute toxicity and impact on cell proliferation and collagen production of fibroblasts

“…Gly, Pro and Ala were dominant hydrophobic amino acids in HC. Hydrophobic amino acids have been found in several antioxidative peptides (Jridi et al ., ; Sae‐Leaw et al ., ; Karnjanapratum et al ., ). Jridi et al .…”

“…Gly, Pro and Ala were dominant hydrophobic amino acids in HC. Hydrophobic amino acids have been found in several antioxidative peptides (Jridi et al, 2014;Sae-Leaw et al, 2016b;Karnjanapratum et al, 2017). Jridi et al (2014) found that the cuttlefish skin hydrolysate with the higher ratio of hydrophobic amino acids had the higher antioxidative activities.…”

Protective effects of thornback ray muscle protein hydrolysate against dyslipidemia, oxidative stress and reduced fertility induced by high cholesterol diet in adult male rats

“…Effectively, several researchers groups, have been able to generate various biological activities from different marine proteins in vitro [7][8][9] and in vivo.…”

Characterization, antioxidative and ACE inhibitory properties of hydrolysates obtained from thornback ray ( Raja clavata ) muscle

“…Protein hydrolysates come as an alternative to synthetic antioxidants. In fact, protein hydrolysates derived from many fish species such as goby [4], cuttlefish [11], sardinella [12] and zebra blenny [13] have demonstrated antioxidative activities.…”