Characterization and Proposed Nomenclature of Epidemic Strains of MRSA in Canada

Simor, A.E.; Boyd, David A.; Louie, Lisa; McGeer, Allison; Mulvey, Michael R.; Willey, BM

doi:10.1155/1999/769601

Cited by 19 publications

(27 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of the strains were typeable with the Basic International Set of Typing Phages for S. aureus and were included in group III. This phage susceptibility pattern, together with a homogeneous methicillin resistance phenotype, has been described for many epidemic strains (42,52).…”

Section: Discussionmentioning

confidence: 64%

Identification of a Novel Methicillin-Resistant Staphylococcus aureus Epidemic Clone in Córdoba, Argentina, Involved in Nosocomial Infections

et al. 2002

View full text Add to dashboard Cite

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are increasingly a main health concern worldwide for hospitalized patients. In addition, the prevalence of community-acquired infection has risen continuously during the last few years. Some MRSA clones spread easier than others within the hospital environment and therefore are frequently implicated in outbreaks. Thus, the spread of a unique epidemic multiresistant clone, the so-called South American clone, is the main cause of nosocomial infections produced by this bacterium in Brazil and in some regions of Argentina, Chile, and Uruguay. In the present work we describe the identification of a novel clone of MRSA that is involved in nosocomial infections and that shows a prevalence as high as that for the South American clone. A total of 53 consecutive single-patient MRSA isolates were recovered during a 3-month period (May to July 1999) from six different hospitals (955 beds) in Córdoba. The isolates were initially typed according the antibiotic resistance and phage susceptibility patterns, followed by genotyping using pulsed-field gel electrophoresis (PFGE). PFGE analysis of the 53 MRSA isolates revealed six major types (A to F) and 25 subtypes. The B-type DNA pattern was indistinguishable from that of the South American epidemic clone observed in 34% of the isolates. A novel highly prevalent clone, showing the A-type DNA pattern and representing 38% of the isolates, was also identified. Moreover, the most frequent subtype of the A clonal family triggered an outbreak in a hospital 2 months later, further confirming its epidemic feature.

show abstract

Section: Discussionmentioning

confidence: 64%

Identification of a Novel Methicillin-Resistant Staphylococcus aureus Epidemic Clone in Córdoba, Argentina, Involved in Nosocomial Infections

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The advent of clinical MRSA isolates displaying reduced susceptibility to vancomycin in numerous countries raises the distinct possibility that vancomycin-resistant MRSA could emerge in patient populations (8,15,18,24). This fact, along with the recent identification of epidemic strains in Canada (16), highlights the need for a rapid molecular-based surveillance mechanism in Canada to aid in infection control efforts. The recent development of server-based data collection systems for fingerprint information (BioNumerics) and the standardization of PFGE methods such as the methods described in this report, will be required to collect and disseminate accurate, timely information to infection control practitioners a Site M results could not be normalized using Molecular Analyst DST software.…”

Section: Assessment Of Protocolsmentioning

confidence: 99%

Development of a Canadian Standardized Protocol for Subtyping Methicillin-Resistant Staphylococcus aureus Using Pulsed-Field Gel Electrophoresis

Chui²,

et al. 2001

View full text Add to dashboard Cite

A panel of 24 methicillin-resistant Staphylococcus aureus strains was distributed to 15 laboratories in Canada to evaluate their in-house pulsed-field gel electrophoresis (PFGE) protocols and interpretation criteria. Attempts to compare fingerprint images using computer-aided analysis were not successful due to variability in individual laboratory PFGE protocols. In addition, individual site interpretation of the fingerprint patterns was inadequate, as 7 of 13 sites (54%) made at least one error in interpreting the fingerprints from the panel. A 2-day standardized PFGE protocol (culture to gel image) was developed and distributed to all of the sites. Each site was requested to use the standardized protocol on five strains from the original panel. Thirteen sites submitted gel images for comparisons. The protocol demonstrated excellent reproducibility and allowed interlaboratory comparisons with Molecular Analyst DST software (Bio-Rad) and 1.5% band tolerance.

show abstract

“…The most common strains of MRSA identified were CMRSA-1 and CMRSA-2, which were also the most prevalent strains in Ontario during the study period (15). Baseline (pretreatment) and follow-up (post-treatment) isolates from patients who failed decolonization therapy demonstrated identical PFGE DNA profiles.…”

Section: Resultsmentioning

confidence: 92%

Combined Topical and Oral Antimicrobial Therapy for the Eradication of Methicillin‐Resistant Staphylococcus aureus (Mrsa) Colonization in Hospitalized Patients

Fung

Louie

Simor

2002

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

OBJECTIVE: How to eradicate methicillin-resistantStaphylo-coccus aureus(MRSA) colonization in hospitalized patients is uncertain. We reviewed our experience with MRSA decolonization therapy in hospitalized patients.SETTING: An 1100-bed, university-affiliated tertiary care teaching hospital in Toronto, Ontario.DESIGN: Retrospective chart review of 207 adult inpatients with MRSA colonization hospitalized between February 1996 and March 1999.INTERVENTIONS: All patients with MRSA colonization were assessed for possible decolonization therapy with a combination of 4% chlorhexidine soap for bathing and washing, 2% mupirocin ointment applied to the anterior nares three times/day, rifampin (300 mg twice daily) and either trimethoprim/sulfamethoxazole (160 mg/800 mg twice daily) or doxycycline (100 mg twice daily). This treatment was given for seven days.RESULTS: A total of 207 hospitalized patients with MRSA colonization were identified and 103 (50%) received decolonization therapy. Patients who received decolonization therapy were less likely than untreated patientsto have intravenous (P=0.004) or urinary catheters (P<0.001), or extranasal sites of colonization (P=0.001). Successful decolonization was achieved in 90% of the 43 patients who were available for at least three months of follow-up.CONCLUSIONS: Combined topical and oral antimicrobial therapy was found to be effective in eradicating MRSA colonization in selected hospitalized patients, especially those without indwelling medical devices or extranasal sites of colonization.

show abstract

Characterization and Proposed Nomenclature of Epidemic Strains of MRSA in Canada

Cited by 19 publications

References 3 publications

Identification of a Novel Methicillin-Resistant Staphylococcus aureus Epidemic Clone in Córdoba, Argentina, Involved in Nosocomial Infections

Identification of a Novel Methicillin-Resistant Staphylococcus aureus Epidemic Clone in Córdoba, Argentina, Involved in Nosocomial Infections

Development of a Canadian Standardized Protocol for Subtyping Methicillin-Resistant Staphylococcus aureus Using Pulsed-Field Gel Electrophoresis

Combined Topical and Oral Antimicrobial Therapy for the Eradication of Methicillin‐Resistant Staphylococcus aureus (Mrsa) Colonization in Hospitalized Patients

Contact Info

Product

Resources

About