Characterization and Quantification of Anthocyanins and Polyphenolics in Blue Honeysuckle (Lonicera caerulea L.)

Abstract

Anthocyanins and phenolics of 10 blue honeysuckle (Lonicera caerulea L.) genotypes were characterized and quantified by HPLC-DAD. Peak assignments were confirmed by low-resolution electrospray mass spectrometry. Six anthocyanins were detected with the major peak identified as cyanidin 3-glucoside. Five additional anthocyanins were characterized as cyanidin 3,5-diglucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, peonidin 3-glucoside, and peonidin 3-rutinoside. Four polyphenolics were identified as chlorogenic acid, neochlorogenic acid, quercetin 3-rutinoside, and quercetin 3-glucoside. Two additional unidentified phenolics were characterized as flavonol and hydroxycinnamic derivatives based on UV-vis spectra. Hydroxycinnamate levels ranged from 30.4 to 156.2 mg/100 g, whereas the flavonol content ranged from 12.6 to 32.8 mg/100 g. The L. caerulea subspecies boczkarnikovae contained the highest amounts of hydroxycinnamic derivatives and flavonols.

Berry Fruits as a Source of Biologically Active Compounds: The Case of Lonicera Caerulea

“…Other hydroxycinnamic acid and hydroxybenzoic acid derivatives are mentioned in the literature, especially m-coumaric and p-coumaric. The amount of hydroxycinnamic acids derivatives is described as comparable to blueberries or blackcurrant 46 . Also dimethoxycinnamic, hydroxycaff eic, gallic, o-pyrocatechuic, protocatechuic, salicylic, p-hydroxyphenylacetic and p-hydroxyphenyllactic acid are reported 45 .…”

“…5). Quantities of these compounds vary depending on a number of the factors 46 . Table 2 shows a comparison of L. caerulea phenolic fraction anthocyanin content with those of Vaccinium macrocarpon 90 and Vitis vinifera 91 .…”

Chemical Composition and Physical Characteristics of Fruits of Two Cultivars of Blue Honeysuckle (Lonicera caerulea L.) in Relation to their Degree of Maturity and Harvest Date

“…Berries have been long harvested from wild plants in regions of Russia, China, and Japan (Thompson and Chaovanalikit, 2006). However, in the scientific literature, the taxonomic classification of the species bearing edible fruits, especially of Lonicera caerulea, is not unequivocal (Chaovanalikit et al, 2004;Plekhanova, 2000). Lonicera caerulea, the blue honeysuckle, is also dubbed honeyberry, sweet berry honeysuckle, edible honeysuckle, haskap, haskup, hasukappu or haskappu.…”

Identificação de antocianidinas em acerolas do Banco Ativo de Germoplasma da Universidade Federal Rural de Pernambuco

“…CHAOVANALIKIT, THOMPSON & WROLSTAD [7], em frutos de "blue honeysuckle" (Lonicera caerulea L.), relataram que seis antocianinas foram separadas por CLAE, utilizando coluna de fase reversa na seguinte ordem de eluição: cianidina 3,5-diglicosídeo, cianidina 3-glicosídeo, cianidina 3-rutinosídeo, pelargonidina 3-glicosídeo, peonidina 3-glicosídeo, peonidina 3-rutinosídeo.…”

“…As técnicas de separação e identificação mais utilizadas estão 1 baseadas em equipamentos sofisticados e, conseqüentemen-te, onerosos como o cromatógrafo líquido de alta eficiência (CLAE), utilizando coluna de fase reversa para separação e identificação por ressonância magnética nuclear [13], arranjo de diodos e espectrometria de massa [7,14] e eletroforese de zona de capilaridade [17].…”