Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

Abstract

The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.

Characterization and selection of inter simple sequence repeat markers for genetic studies of mesquite

“…These authors reached He and PIC values from 0 to 0.38 and from 0 to 0.29, respectively. Although lower than our findings on He and PIC, of the 17 RGA primers tested bySantos et al (2021b), 12 were highly polymorphic and can be used to investigate genetic variability in further studies on mesquite. Almeida-Pereira et al(2017) also proved the effectiveness of ISSR markers to estimate genetic variability among accessions of the species C.…”

“…These polymorphism values found by these authors were higher than those obtained in our study. Furthermore, similar characterizations of P. juliflora made with primers analogous to resistance genes (RGA) have generated an average of 5.7 markers per primer (Santos et al, 2021b). Research, Society and Development, v. 11, n. 9, e9011930070, 2022 (CC BY 4.0) In this study, expected heterozygosity (He) for the 23 ISSR primers ranged from 0.15 (TriCAC primer 3'YC) to 0.48 (TriCAC primer 5'CY), averaging 0.28 (Table 1).…”

“…Given the importance of molecular markers and especially the scarcity of available primers, criteria and studies dedicated to the prior selection of primers should be prioritized. In this sense, some studies in the literature may be highlighted, for instance those in Croton tetradenius Baill (Almeida-Pereira et al, 2017) and Zea mays L. (Muhammad et al, 2017), as well as those on selection of markers analogous to resistance genes in P. juliflora (Santos et al, 2021b).…”

“…) described the importance of studies on amplification of ISSR markers, as monomorphic percentages may vary from one sample to another. Moreover,Santos et al (2021b) proved the efficiency of RGA markers in a pioneering study on characterization and selection in P. juliflora. These authors reached He and PIC values from 0 to 0.38 and from 0 to 0.29, respectively.…”