Characterization and sequence of the Escherichia coli panBCD gene cluster

Merkel, William K.; Nichols, Brian P.

doi:10.1111/j.1574-6968.1996.tb08488.x

Cited by 39 publications

(19 citation statements)

References 11 publications

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“…Lastly, the SL library contains a sequence (SL9) with 83% homology over its full length to the E. coli gene panD encoding aspartate-1-decarboxylase which catalyses synthesis of b-alanine, a precursor of both pantothenate (vitamin B 3 ) and coenzyme-A biosynthesis (Merkel and Nickols 1996). As no S. typhimurium homologs of this sequence were found in databases, it is reasonable to conclude that SL13 is a part of the S. typhimurium panD gene.…”

Section: Sequences Present In Both Genomesmentioning

confidence: 98%

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization

et al. 1999

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The availability of bacterial genome sequences raises an important new problem - how can one move from completely sequenced microorganisms as a reference to the hundreds and thousands of other strains or isolates of the same or related species that will not be sequenced in the near future? An efficient way to approach this task is the comparison of genomes by subtractive hybridization. Recently we developed a sensitive and reproducible subtraction procedure for comparison of bacterial genomes, based on the method of suppression subtractive hybridization (SSH). In this work we demonstrate the applicability of subtractive hybridization to the comparison of the related but markedly divergent bacterial species Escherichia coli and Salmonella typhimurium. Clone libraries representing sequence differences were obtained and, in the case of completely sequenced E. coli genome, the differences were directly placed in the genome map. About 60% of the differential clones identified by SSH were present in one of the genomes under comparison and absent from the other. Additional differences in most cases represent sequences that have diverged considerably in the course of evolution. Such an approach to comparative bacterial genomics can be applied both to studies of interspecies evolution - to elucidate the "strategies" that enable different genomes to fit their ecological niches - and to development of diagnostic probes for the rapid identification of pathogenic bacterial species.

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Section: Sequences Present In Both Genomesmentioning

confidence: 98%

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization

et al. 1999

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“…b-Alanine biosynthesis in plants is not well studied; however, in Escherichia coli the major route for b-alanine biosynthesis is via the a-decarboxylation of L-aspartate catalyzed by the panD-encoded L-aspartate-a-decarboxylase (AspDC, Williamson and Brown 1979;Merkel and Nichols 1996). This route is unique to prokaryotes and absent in eukaryotes including yeast and plants (Rathinasabapathi et al 2000;White et al 2001).…”

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confidence: 99%

Transplastomic expression of bacterial l-aspartate-α-decarboxylase enhances photosynthesis and biomass production in response to high temperature stress

Fouad

Altpeter

2009

Transgenic Res

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Metabolic engineering for beta-alanine over-production in plants is expected to enhance environmental stress tolerance. The Escherichia coli L-aspartate-alpha-decarboxylase (AspDC) encoded by the panD gene, catalyzes the decarboxylation of L-aspartate to generate beta-alanine and carbon dioxide. The constitutive E. coli panD expression cassette was co-introduced with the constitutive, selectable aadA expression cassette into the chloroplast genome of tobacco via biolistic gene transfer and homologous recombination. Site specific integration of the E. coli panD expression cassette into the chloroplast genome and generation of homotransplastomic plants were confirmed by PCR and Southern blot analysis, respectively, following plant regeneration and germination of seedlings on selective media. PanD expression was verified by assays based on transcript detection and in vitro enzyme activity. The AspDC activities in transplastomic plants expressing panD were drastically increased by high-temperature stress. beta-Alanine accumulated in transplastomic plants at levels four times higher than in wildtype plants. Analysis of chlorophyll fluorescence on plants subjected to severe heat stress at 45 degrees C under light verified that photosystem II (PSII) in transgenic plants had higher thermotolerance than in wildtype plants. The CO(2) assimilation of transplastomic plants expressing panD was more tolerant to high temperature stress than that of wildtype plants, resulting in the production of 30-40% more above ground biomass than wildtype control. The results presented indicate that chloroplast engineering of the beta-alanine pathway by over-expression of the E. coli panD enhances thermotolerance of photosynthesis and biomass production following high temperature stress.

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“…The panC gene encodes a pantothenate synthetase (PS), which catalyzes the last step of pantothenate biosynthesis, the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. The pantothenate biosynthetic pathway has been best characterized in E. coli [56]. The crystal structure of E. coli PS was found to be a dimer from the crystal structure [57], although earlier studies indicated that it could be a tetramer in solution [58].…”

Section: Pantothenate Synthetase (Ps) the Product Of Panc (Rv3602c)mentioning

confidence: 98%

High Throughput Crystallography of TB Drug Targets

Murillo¹,

Li²,

Alber³

et al. 2007

IDDT

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Tuberculosis (TB) infects one-third of the world population. Despite 50 years of available drug treatments, TB continues to increase at a significant rate. The failure to control TB stems in part from the expense of delivering treatment to infected individuals and from complex treatment regimens. Incomplete treatment has fueled the emergence of multi-drug resistant (MDR) strains of Mycobacterium tuberculosis (Mtb). Reducing non-compliance by reducing the duration of chemotherapy will have a great impact on TB control. The development of new drugs that either kill persisting organisms, inhibit bacilli from entering the persistent phase, or convert the persistent bacilli into actively growing cells susceptible to our current drugs will have a positive effect. We are taking a multidisciplinary approach that will identify and characterize new drug targets that are essential for persistent Mtb. Targets are exposed to a battery of analyses including microarray experiments, bioinformatics, and genetic techniques to prioritize potential drug targets from Mtb for structural analysis. Our core structural genomics pipeline works with the individual laboratories to produce diffraction quality crystals of targeted proteins, and structural analysis will be completed by the individual laboratories. We also have capabilities for functional analysis and the virtual ligand screening to identify novel inhibitors for target validation. Our overarching goals are to increase the knowledge of Mtb pathogenesis using the TB research community to drive structural genomics, particularly related to persistence, develop a central repository for TB research reagents, and discover chemical inhibitors of drug targets for future development of lead compounds.

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Characterization and sequence of the Escherichia coli panBCD gene cluster

Cited by 39 publications

References 11 publications

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization

Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization

Transplastomic expression of bacterial l-aspartate-α-decarboxylase enhances photosynthesis and biomass production in response to high temperature stress

High Throughput Crystallography of TB Drug Targets

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