Characterization and simultaneous quantification of multiple constituents in Aurantii Fructus Immaturus extracts by HPLC-DAD-ESI-MS/MS

Liu, Wenyuan; Zhou, Chen; Yan, Cuimin; Xie, S. L.; Feng, Feng; Wu, Chunyong; Xie, Ning

doi:10.1016/s1875-5364(12)60087-9

Cited by 11 publications

(14 citation statements)

References 26 publications

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“…Interestingly, due to the difference of the chemical structures between neohesperidin and hesperidin, a series of fragment ions at m/z 489 [M-H-120] − , 343 [M-H-120-rhamnosyl] − , 325 [MH-120-rhamnosyl-H 2 O] − and 301 [M-H-neohesperidosyl] − were observed in MS 2 spectrum of neohesperidin. The MS behavior was consistent with that reported in literatures [19-21]. …”

Section: Resultssupporting

confidence: 91%

“…Other fragment ions at m/z 165, 177 and 227 [M-H-rutinosyl-CO 2 ] − were also observed. This peak was tentatively identified as narirutin according to the MS behavior reported in literature [18-21]. HRMS measurement of the deprotonated ion [M-H] − of peak 3 was 579.1703, also suggesting the chemical composition of C 27 H 31 O 14 (-0.918 ppm).…”

Section: Resultsmentioning

confidence: 60%

“…In MS 3 experiment, the ion at m/z 285 produced fragmentation ions at m/z 270 [M-H-neohesperidosyl-CH 3 ] − , 243 [M-H-neohesperidosyl-C 2 H 2 O] − , 164, and 151. This peak was tentatively identified as poncirin (isosakuranetin-7-neohesperidose) according to literature [20, 21, 24]. …”

Section: Resultsmentioning

confidence: 99%

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Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using flow-injection mass spectrometric (FIMS) metabolic fingerprinting method combined with chemometrics

Zhao

Chang

Chen

2015

Journal of Pharmaceutical and Biomedical Analysis

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A flow-injection mass spectrometric metabolic fingerprinting method in combination with chemometrics was used to differentiate Aurantii Fructus Immaturus from its counterfeit Poniciri Trifoliatae Fructus Immaturus. Flow-injection mass spectrometric (FIMS) fingerprints of 9 Aurantii Fructus Immaturus samples and 12 Poniciri Trifoliatae Fructus Immaturus samples were acquired and analyzed using principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA). The authentic herbs were differentiated from their counterfeits easily. Eight characteristic components which were responsible for the difference between the samples were tentatively identified. Furthermore, three out of the eight components, naringin, hesperidin, and neohesperidin, were quantified. The results are useful to help identify the authenticity of Aurantii Fructus Immaturus.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 60%

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Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using flow-injection mass spectrometric (FIMS) metabolic fingerprinting method combined with chemometrics

Zhao

Chang

Chen

2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Rat PC12 cells were obtained from the American Tissue Culture Collection (Rockville, MD, USA), and were maintained in Dulbecco's modified Eagle medium supplemented with penicillin (100 units/mL), streptomycin (100 μg/mL), 5% fetal bovine serum, and 5% horse serum at 37 • C in humid atmosphere with 5% CO 2 . To evaluate the neuroprotective effect of each compound, PC12 cells were divided into the following eight groups for different treatments: untreated control, 10 μM Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) , 10 μM Aβ (25-35) + 10 μM donepezil hydrochloride, 10 μM Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) + compound (10, 25, 50, 100, 200 μg/mL), n = 6 per group in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The cultures were refreshed with the corresponding serum-free medium 48 h after the cells were seeded, and were further incubated for 48 h. Cell viability was measured using the MTT assay based on the conversion of MTT to formazan crystals by mitochondrial dehydrogenases.…”

Section: Pc12 Cell Cultures and Evaluation Of Cell Viabilitymentioning

confidence: 99%

“…Moreover, PC12 screening systems can be used for cell-based high-throughput screening assays. The Aβ peptide is known to play a central role in the pathogenesis of AD [17]; moreover, an 11-amino acid fragment of the Aβ peptide (Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) ) could induce neuronal damage in PC12 cells. These features render PC12 cells suitable for AD research [18].…”

Section: Introductionmentioning

confidence: 99%

Extraction and isolation of acetylcholinesterase inhibitors from Citrus limon peel using an in vitro method

Liu

Hou

et al. 2020

J of Separation Science

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A simple and efficient ultrafiltration-liquid chromatography-mass spectrometrybased method was developed for the rapid screening and identification of ligands from Citrus limon peel, which are suitable acetylcholinesterase inhibitors. Subsequently, the anti-Alzheimer's activity of these compounds was assessed using a PC12 cell model. Six major compounds, viz. neoeriocitrin, isonaringin, naringin, hesperidin, neohesperidin, and limonin, were identified as potent acetylcholinesterase inhibitors.A continuous and efficient online method, which involved the use of a microwaveassisted extraction device, solvent concentration tank, and centrifugal partition chromatography column, was developed for the scale-up of these compounds, and the obtained compounds presented high purity. Next, their bioactivity was evaluated using a PC12 cell model. This novel approach, which was based on ultrafiltration-liquid chromatography-mass spectrometry, microwave-assisted extraction online coupled with solvent concentration tank, and centrifugal partition chromatography along with in vitro evaluation, could represent a powerful tool for the screening and extraction of acetylcholinesterase inhibitors from complex matrices, and could be a useful platform for the large-scale production of bioactive and nutraceutical ingredients.

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Characterization of chemical constituents in Zhi–Zi–Da–Huang decoction by ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Zhu

Yin

Han

et al. 2014

J of Separation Science

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A sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to separate and identify the chemical constituents of Zhi-Zi-Da-Huang decoction, a classic traditional Chinese medicine formula. The chromatographic separation was achieved on a Shim-pack XR-ODS C18 column (75 × 3.0 mm, 2.2 μm) using a gradient elution program. The detection was performed on a Waters Xevo G2 Q-TOF mass spectrometer equipped with electrospray ionization source in both positive and negative modes. With the optimized conditions, a total of 82 compounds were identified or tentatively characterized. Of the 82 compounds, 21 compounds were identified by comparing the retention time and MS data with reference standards, the rest were characterized by analyzing MS data and retrieving the reference literature. In addition, 31 compounds were identified from Gardenia jasminoides Ellis, ten compounds were identified from Rheum palmatum L., 33 compounds were identified from Citrus aurantium L., and eight compounds were identified from Sojae Semen Praeparatum. Results indicated that iridoids, anthraquinones, flavonoids, isoflavonoids, coumarins, glycosides of crocetin, monoterpenoids, and organic acids were major constituents in Zhi-Zi-Da-Huang decoction. It is concluded that the developed ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method with high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents of Zhi-Zi-Da-Huang decoction, and the analysis provides a helpful chemical basis for further research on Zhi-Zi-Da-Huang decoction.

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Characterization and simultaneous quantification of multiple constituents in Aurantii Fructus Immaturus extracts by HPLC-DAD-ESI-MS/MS

Cited by 11 publications

References 26 publications

Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using flow-injection mass spectrometric (FIMS) metabolic fingerprinting method combined with chemometrics

Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using flow-injection mass spectrometric (FIMS) metabolic fingerprinting method combined with chemometrics

Extraction and isolation of acetylcholinesterase inhibitors from Citrus limon peel using an in vitro method

Characterization of chemical constituents in Zhi–Zi–Da–Huang decoction by ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

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