Characterization and Use of Isolated Toadfish Hepatocytes for Studies of Heme Synthesis and Utilization

Cornell, Neal W.; Hahn, M.; Martin, Holger

doi:10.1086/bblv189n2p227

Cited by 3 publications

(2 citation statements)

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“…These factors are nutritional status and fish species, the isolation of enzymes and the enzymes used during the treatment duration. In various studies on fish, hepatocyte isolation as a result in number of cells g À1 was determined as follows: Opsanus tau 3.4 910 8 (Cornell, Hahn & Martin 1995); Japanese eel (Anguilla japonica), 2.66 AE 1.10 9 10 8 (Yu, Ando & Hayashi 1991); and tilapia (Oreochromis mossambicus), 34-88 9 10 6 (Kim & Takemura 2002). The mean cell number was determined as 10.26 AE 5.80 9 10 7 in the result of hepatocytes isolation from C. tarichi.…”

Section: Discussionmentioning

confidence: 99%

In vitroeffect of pituitary, interrenal and gonadal hormones on vitellogenin synthesis in primary hepatocyte cultures ofChalcalburnus tarichi

Oğuz

Ünal

2013

Aquac Res

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Summary Vitellogenin (Vtg) is an important precursor yolk protein in egg‐laying vertebrates, including fish. The 17β‐oestradiol (E2) plays a crucial role in the Vtg synthesis; moreover, certain hormones can stimulate Vtg synthesis. We investigated the possible role of E2, carp recombinant growth hormone (crGH), insulin (Ins), progesterone (P4) and 11‐deoxycortisol (11‐DOC) hormones in Vtg synthesis on primary juvenile Chalcalburnus tarichi hepatocyte culture. The amount of Vtg in the medium was measured at 2‐day intervals using enzyme‐linked immunosorbent assay (ELISA). The hepatocytes were maintained in culture for more than 2 weeks without the addition of serum components. Vitellogenin localization was visualized with the immunofluorescence method in E2‐supplemented hepatocytes. Among hormones applied to the culture, only E2 had an influence on Vtg synthesis in a time‐dependent manner, while crGH, Ins, P4 and 11‐DOC had no effect. However, in hepatocytes stimulated with E2 in combination with P4, a lower Vtg production was seen compared with Vtg produced when hepatocytes were stimulated with E2 alone. P4 proved to have potentiating effects on co‐treatment with E2‐induced Vtg production. As a result, E2 and P4 are the most important hormones for Vtg synthesis in juvenile C. tarichi hepatocyte culture.

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Section: Discussionmentioning

confidence: 99%

In vitroeffect of pituitary, interrenal and gonadal hormones on vitellogenin synthesis in primary hepatocyte cultures ofChalcalburnus tarichi

Oğuz

Ünal

2013

Aquac Res

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show abstract

“…As mentioned above, the sensitivity of the radiochemical assay makes it valuable in studies of tissues or cells with low content of ALA synthase. One example of that is a preliminary study of induction of the enzyme by succinyl acetone or polychlorinated biphenyls in isolated fish hepatocytes in which basal activity is only 15 to 20 U/g of wet weight of cells (Cornell et al, 1995).…”

Section: Commentary Background Informationmentioning

confidence: 99%