Characterization and validation of a bioluminescent bioreporter for the direct detection of Escherichia coli

Birmele, Michele N.; Ripp, Steven; Jegier, Patricia; Roberts, Michael S.; Sayler, Gary S.; Garland, Jay L.

doi:10.1016/j.mimet.2008.06.003

Cited by 19 publications

(12 citation statements)

References 19 publications

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“…Another interesting concept is the use of luxI, one regulatory gene of the bioluminescence cassette of Vibrio fischeri, as a reporter gene. 58,[79][80][81] The luxI gene encodes a synthase of acyl-homoserine lactone (AHL), which functions as diffusible quorum sensing molecule that induces transcription of the lux operon resulting in bioluminescence of neighboring cells carrying the essential genes. By using an engineered luxI incorporated lambda phage and an E. coli lux bioreporter strain, the authors were able to detect as few as 1 E. coli cell per ml in pure culture within slightly more than 10 h and 130 CFU/ml in less than 24 h in artificially contaminated lettuce leaf washings.…”

Section: Genetically Modified Reporter Phagementioning

confidence: 99%

Application of bacteriophages for detection of foodborne pathogens

2014

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Bacterial contamination of food products presents a challenge for the food industry and poses a high risk for the consumer. Despite increasing awareness and improved hygiene measures, foodborne pathogens remain a threat for public health, and novel methods for detection of these organisms are needed. Bacteriophages represent ideal tools for diagnostic assays because of their high target cell specificity, inherent signal-amplifying properties, easy and inexpensive production, and robustness. Every stage of the phage lytic multiplication cycle, from the initial recognition of the host cell to the final lysis event, may be harnessed in several ways for the purpose of bacterial detection. Besides intact phage particles, phage-derived affinity molecules such as cell wall binding domains and receptor binding proteins can serve for this purpose. This review provides an overview of existing phage-based technologies for detection of foodborne pathogens, and highlights the most recent developments in this field, with particular emphasis on phage-based biosensors.

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Section: Genetically Modified Reporter Phagementioning

confidence: 99%

Application of bacteriophages for detection of foodborne pathogens

2014

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“…The well-characterized bacterial luciferase genes, encoded by the lux operon from the aquatic Gram-negative Vibrio fischeri are perhaps the most frequently used reporters for incorporation into recombinant phages and have many applications, namely for acute toxicity prediction for chemicals [92]. Other-component bacteriophage-based bioluminescent reporter systems were developed for the detection of Escherichia coli in environmental samples, for instance [93]. …”

Section: Optical Monitoring Of Microbial Contamination: Current Mementioning

confidence: 99%

Microbial Contamination Detection in Water Resources: Interest of Current Optical Methods, Trends and Needs in the Context of Climate Change

Jung

Cann

Roig

et al. 2014

IJERPH

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Microbial pollution in aquatic environments is one of the crucial issues with regard to the sanitary state of water bodies used for drinking water supply, recreational activities and harvesting seafood due to a potential contamination by pathogenic bacteria, protozoa or viruses. To address this risk, microbial contamination monitoring is usually assessed by turbidity measurements performed at drinking water plants. Some recent studies have shown significant correlations of microbial contamination with the risk of endemic gastroenteresis. However the relevance of turbidimetry may be limited since the presence of colloids in water creates interferences with the nephelometric response. Thus there is a need for a more relevant, simple and fast indicator for microbial contamination detection in water, especially in the perspective of climate change with the increase of heavy rainfall events. This review focuses on the one hand on sources, fate and behavior of microorganisms in water and factors influencing pathogens’ presence, transportation and mobilization, and on the second hand, on the existing optical methods used for monitoring microbiological risks. Finally, this paper proposes new ways of research.

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“…The target cells that contain these bioluminescence genes can then be detected with a luminometer. As bioluminescence genes, the complete lux operon can be used, but also a part of this lux operon, like luxI (Birmele et al, 2008;Ripp et al, 2006Ripp et al, , 2008 or luxAB can be used (Chen and Griffiths, 1996;Kodikara et al, 1991;Loessner et al, 1997). With bioluminescence phage biosensors, it is possible to determine rapidly low concentration of food pathogens compared to standard plating methods, taking approximately four days.…”

Section: Transductionmentioning

confidence: 99%