Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract

Abstract

Recently, major problem related to pathogenic bacteria is augmentation of antibiotic resistance which has been changed treatment and recovery of millions of infectious patients. The present study reports an eco-friendly, rapid and easy method for synthesis of silver (Ag), copper (Cu) and titanium dioxide (TiO) nanoparticles (NPs) using Artemisia haussknechtii leaf aqueous extract with antibacterial activities against multi-drug resistance (MDR) bacteria species. Three different concentrations (0.001, 0.01 and 0.1 M) of AgNO, CuSO and TiO (OH) were investigated for obtaining optimum NPs green synthesis. Total phenolic content, total flavonoid content of leaf extract and total antioxidant activity (DPPH) assay were determined as radical scavenging methods. UV-Visible spectroscopy, Fourier transform infrared spectroscopy analysis, X-ray diffraction, energy dispersive X-ray spectroscopy, field emission scanning electron microscope and atomic force microscopy (AFM) were used due to NPs characterization. The size average of the Ag, Cu and TiO NPs obtained were respectively 10.69 ± 5.55, 35.36 ± 44.4 and 92.58 ± 56.98 nm. In the case of antibacterial assay, disc diffusion assay, minimum inhibitory concentration, minimum bactericidal concentration, bacterial growth and morphology of four MDR species Staphylococcus aureus ATCC 43300, Staphylococcus epidermidis ATCC 12258, Serratia marcescens ATTC13880 and Escherichia coli ATCC 25922 were evaluated. Results of this study demonstrated that A. haussknechtii leaf extract with various groups of phytochemicals such as phenols and flavonoids had suitable ability in green synthesis of Ag, Cu and TiO NPs. Also, Ag and Cu NPs had more antibacterial activities compared to TiO NPs.

Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity

“…Thus, for silver the crystallite size was 69(23) Å, and for manganese was 11.953230 Å. Our results are consistent with previous findings [1,25,30,31,[46][47][48]. However, additional peaks were observed, and they could be associated to Ag2O at 2θ values of 27°, 32°, and 44° (PDF card no.…”

“…In this field, the progress of nanotechnology led to the development of nanoparticles (NPs), as contrast agents or in targeted treatments. The NPs are defined as nanoscaled structures that do not exceed 100 nm in size and have physical, chemical, and biological properties that differ from those of the bulk material, primarily due to an increased surface to volume ratio of the former [1,2]. Due to their importance in clinical applications, the NPs are intensively investigated, and major progress was made in recent years on green synthesis techniques of nanomaterials and on assessing their biological effects on living organisms [3,4].…”

Multifunctional fluorescent titania nanoparticles: green preparation and applications as antibacterial and cancer theranostic agents

“…According to the MBC and growth curve results, the HTN showed a higher antibacterial activity against gram-negative (E. coli) than gram-positive (S. aureus) bacteria. The less susceptibility of S. aureus might be related to its thick peptidoglycan layer (20-80 nm) compared to thin layer of E. coli (5-10 nm) [40] which may protect formation of pits by ROS [34].…”

Effect of Incubation Time, CuSO4 and Glucose Concentrations on Biosynthesis of Copper Oxide (CuO) Nanoparticles with Rectangular Shape and Antibacterial Activity: Taguchi Method Approach

“…In return, NPs synthesis through biological techniques specifically applying microorganisms emerged as an eco-friendly, clean and benefit alternative to physicochemical techniques [23]. In recent years, green methods have attained tremendous attention in synthesis of pure or oxide MNPs [24][25][26][27]. However, not real green nature of these methods is a major problem [28].…”