Characterization, antimicrobial, and antioxidant potentialities of first-time isolated silver nanoparticles synthesizing protein secreted by Lysinibacillus sphaericus

Liu, Pei; Wen-Hao, Zhao; Yang-Ping, Song; Wang, Qiang; Yifan, Kan; Wang, Shiyan; Xia, Jilin; Bilal, Muhammad; Zhu, Xiaoyan; Wang, Zhongxing; Sun, Min; Li, Xiangqian; He, Shuai

doi:10.1016/j.procbio.2022.08.032

Cited by 8 publications

(8 citation statements)

References 32 publications

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“…This result was consistent with those reported for a number of Bacillus species, including B. subtilis [ 46 ], B. licheniformis [ 47 ], B. cereus [ 48 ], and B. thuringiensis [ 49 ]. Liu et al [ 50 ] showed that they synthesized AgNPs by using Lysinibacillus sphaericus after 24 h. On the other hand, Nallal et al [ 51 ] succeeded in the biosynthesis of AgNPs by using 1 mM of silver nitrate mixed with an A. ampeloprasum bulb extract within 20 min. Additionally, after 24 h of incubation, the green production of AgNPs utilizing A. sydowii was accomplished [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

“…strain AW1-2, the absorption peak of UV–Vis spectroscopy was detected at 447 nm for the biogenesis of AgNPs [ 44 ]. Moreover, Liu et al [ 50 ] reported that the UV−Vis spectra of the biosynthesized AgNPs by using Lysinibacillus sphaericus showed a prominent absorbance peak at 413 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Elbahnasawy et al [ 71 ] discovered that FTIR spectrum analysis suggested that the cell-free extract of Rothia endophytica MAE 11 may function as a reducing and stabilizing agent during the production of AgNPs. Liu et al [ 50 ] showed that FTIR spectra of Lysinibacillus sphaericus mediated the synthesis of AgNPs to identify biomolecules involved in the reduction of Ag + ions and capping of biosynthesized AgNPs. Almalki and a Khalifa [ 42 ] employed FTIR spectra to identify biomolecules found in the CFE of Bacillus sp KFU36, which were used as reducing agents for AgNP synthesis.…”

Section: Resultsmentioning

confidence: 99%

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Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC50 = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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“…The HPLC provides information regarding the composition of the biological agent used in the green synthesis. Different studies have suggested that proteins present in the cell-free supernatants obtained from microbial cultures can act as coating agents [ 93 , 94 , 95 ]. The proteins implicated in the functionalization of NPs can be identified and characterized by electrophoresis, immunoblotting or sequencing.…”

Section: Most Common Techniques Used For Characterization Of Biologic...mentioning

confidence: 99%

New Green Approaches in Nanoparticles Synthesis: An Overview

Miu

Dinischiotu

2022

Molecules

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Nanotechnology is constantly expanding, with nanomaterials being more and more used in common commercial products that define our modern life. Among all types of nanomaterials, nanoparticles (NPs) occupy an important place, considering the great amount that is produced nowadays and the diversity of their applications. Conventional techniques applied to synthesize NPs have some issues that impede them from being appreciated as safe for the environment and health. The alternative to these might be the use of living organisms or biological extracts that can be involved in the green approach synthesis of NPs, a process that is free of harmful chemicals, cost-effective and a low energy consumer. Several factors, including biological reducing agent concentration, initial precursor salt concentration, agitation, reaction time, pH, temperature and light, can influence the characteristics of biologically synthesized NPs. The interdependence between these reaction parameters was not explored, being the main impediment in the implementation of the biological method on an industrial scale. Our aim is to present a brief review that focuses on the current knowledge regarding how the aforementioned factors can control the size and shape of green-synthesized NPs. We also provide an overview of the biomolecules that were found to be suitable for NP synthesis. This work is meant to be a support for researchers who intend to develop new green approaches for the synthesis of NPs.

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“…Interestingly, the detected MIC value of biogenic AgNPs against E. coli strain was lower than that detected in a prior report which demonstrated MIC value of 300 μg/mL indicating the high efficiency of synthesis method followed in our current investigation. 54 The biogenic silver nanomaterials utilize several mechanisms of actions, contributing to their potent antibacterial effectiveness. Here are four of AgNPs’ documented antibacterial effects: (1) adherence to the microbial membrane’s surface, (2) AgNPs disrupt cellular biomolecules after internalization into bacterial cells resulting in intracellular damage, (3) they cause cellular toxicity by producing reactive oxygen species (ROS) that create oxidative stress in the cell, and (4) they interfere with the cell’s signal transduction pathways.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Antibacterial Potential of Greenly Synthesized Silver Nanoparticles with Fosfomycin Against Some Nosocomial Bacterial Pathogens

Aljeldah

Yassin

Mostafa

et al. 2023

IDR

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Introduction A considerable number of morbidities and fatalities occur worldwide as a result of the multidrug resistant microorganisms that cause a high prevalence of nosocomial bacterial infections. Hence, the current investigation was conducted to evaluate the antibacterial potency of green fabricated silver nanoparticles (AgNPs) against four different nosocomial pathogens. Methods The flower extract of Hibiscus sabdariffa mediated green fabrication of AgNPs and their physicochemical features were scrutinized using different techniques. Antimicrobial activity of the biogenic AgNPs and their synergistic patterns with fosfomycin antibiotic were evaluated using disk diffusion assay. Results and Discussion UV spectral analysis affirmed the successful formation of AgNPs through the detection of broad absorption band at 395 and 524 nm, indicating the surface plasmon resonance of the biofabricated AgNPs. In this setting, the biofabricated AgNPs demonstrated average particle size of 58.682 nm according to transmission electron microscope (TEM) micrographs. The detected hydrodynamic diameter was higher than that noticed by TEM analysis, recording 72.30 nm in diameter and this could be attributed to the action of capping agents, which was confirmed by Fourier Transform Infrared (FT-IR) analysis. Disk diffusion assay indicated the antibacterial potency of biogenic AgNPs (50 μg/disk) against Enterobacter cloacae , Methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli strains with relative inhibition zone diameters of 12.82 ± 0.36 mm, 14.54 ± 0.15 mm, 18.35 ± 0.24 mm and 21.69 ± 0.12 mm, respectively. In addition, E. coli was found to be the most susceptible strain to the biogenic AgNPs. However, the highest synergistic pattern of AgNPs-fosfomycin combination was detected against K. pneumonia strain recording relative synergistic percentage of 64.22%. In conclusion, the detected synergistic efficiency of AgNPs and the antibiotic fosfomycin highlight the potential for utilizing this combination in the biofabrication of effective antibacterial agents against nosocomial pathogens.

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Characterization, antimicrobial, and antioxidant potentialities of first-time isolated silver nanoparticles synthesizing protein secreted by Lysinibacillus sphaericus

Cited by 8 publications

References 32 publications

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

New Green Approaches in Nanoparticles Synthesis: An Overview

Synergistic Antibacterial Potential of Greenly Synthesized Silver Nanoparticles with Fosfomycin Against Some Nosocomial Bacterial Pathogens

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