Characterization, Cytotoxic Analysis and Action Mechanism of Antilisterial Bacteriocin Produced by Lactobacillus plantarum Isolated from Cheddar Cheese

Ibrahim, Fariha; Siddiqui, Nadir Naveed; Aman, Afsheen; Qader, Shah Ali Ul; Ansari, Asma

doi:10.1007/s10989-019-09982-5

Cited by 15 publications

(17 citation statements)

References 44 publications

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“…Scanning electron micrographs clearly showed deformation and damage of cell morphology, thus confirming the bactericidal nature of purified bacteriocins. Previous studies on bacteriocins, such as BAC-IB17 and Bac-IB45 demonstrated bactericidal action on the indicator strains that may be due to increase in membrane permeability that leads to pore formation and subsequently causes cell death (Ansari et al 2018;Ibrahim et al 2020). The above data supported the results of our study.…”

Section: Discussionsupporting

confidence: 92%

“…Another recent report on bacteriocin BAC-IB17 showed anticancer activity on HeLa cells but no cytotoxicity was found against normal cells and showed 90% of cell viability (Ansari et al 2020). Similarly, BacIB45 produced by Lactobacillus plantarum revealed 85% cell viability at inhibitory concentration after bacteriocin treatment (Ibrahim et al 2020), which supported this study. Few earlier studies also reported further characterized bacteriocins with less potential activity than bacteriocins of our present interest.…”

Section: Discussionsupporting

confidence: 86%

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Characterization and Cytotoxic Evaluation of Bacteriocins Possessing Antibiofilm Activity Produced by Lactobacillus plantarum SJ33

Mohapatra

Lakshmanan

Mahesh

et al. 2021

Int J Pept Res Ther

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Biofilm forming pathogens are among the major causes of hospital-acquired infections and are not much affected by antibiotic treatment. Consequently, novel agents and therapeutics are required urgently that possess antibacterial and antibiofilm activities. This study analyzed two bacteriocins from Lactobacillus plantarum subsp. argentoratensis SJ33 strain for their antibacterial and antibiofilm activity as well as cytotoxic properties. BacF1 and BacF2 showed broad spectrum activity against both Gram-positive (Listeria monocytogenes, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. Significant bactericidal action was also observed on S. aureus cells by pore formation. Additionally, bacteriocins disrupted biofilms formed by S. aureus and P. aeruginosa which were shown by crystal violet staining assay and visualized by fluorescence as well as scanning electron microscopy. Quantitative Real-Time PCR study revealed changes in gene expression of biofilm formation in S. aureus (ica) and P. aeruginosa (pelA, psl, rhlA). Cytotoxicity of bacteriocins was further analyzed on normal mammalian cells and Caenorhabditis elegans. Notably, bacteriocins showed no major effect on HEK-293 cell line and enhanced the survival of S. aureus infected HEK-293 cells. Similarly, no cytotoxic effect was visible on C. elegans even after treatment with higher concentration than MIC at different time intervals.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 86%

Characterization and Cytotoxic Evaluation of Bacteriocins Possessing Antibiofilm Activity Produced by Lactobacillus plantarum SJ33

Mohapatra

Lakshmanan

Mahesh

et al. 2021

Int J Pept Res Ther

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“…The stability of bacteriocins is crucial for their application, and although thermostability is well reported (Ansari, Zohra, Tarar, Qader, & Aman, 2018;Elyass, Mahdi, & Attitalla, 2015), it is reduced when there is the retention of activity in autoclaving conditions, as presented by Gluconacin. Other studies have also reported this characteristic in several bacteriocins (Ibrahim, Siddiqui, Aman, Qader, & Ansari, 2020;Qiao et al, 2020;Yi, Qi, Hong, Deng, & Zeng, 2020). Such properties are important and open new perspectives for large-scale production of Gluconacin.…”

Section: Discussionmentioning

confidence: 69%

Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

Ramos

Meneses

Vidal

et al. 2021

Annals of Applied Biology

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Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 αhelices and 11 β-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 μg μl À1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials.Scanning electron microscopy images showed that Gluconacin-treated X. albilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis.Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.

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“…Bacteriocin of L. plantarum KIBGE IB45 was found to be active against numerous multi-drug resistant & food-borne pathogen especially L. monocytogens. In-vitro cytotoxicity showed that Bac-ib45 was non cytotoxic protein (Ibrahim et al, 2019). The results of current investigation revealed that bacteriocin was approximately 42 fold purified with the molecular weight of approximately 20 kDa assessed by Tricin SDS PAGE.…”

Section: O N C L U S I O N S / R E S U L T Smentioning

confidence: 74%

Characterization and In Vitro Application of Lactobacillus plantarum Bacteriocin as Biopreservative

Ansari¹

2021

Ibras

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Characterization, Cytotoxic Analysis and Action Mechanism of Antilisterial Bacteriocin Produced by Lactobacillus plantarum Isolated from Cheddar Cheese

Cited by 15 publications

References 44 publications

Characterization and Cytotoxic Evaluation of Bacteriocins Possessing Antibiofilm Activity Produced by Lactobacillus plantarum SJ33

Characterization and Cytotoxic Evaluation of Bacteriocins Possessing Antibiofilm Activity Produced by Lactobacillus plantarum SJ33

Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

Characterization and In Vitro Application of Lactobacillus plantarum Bacteriocin as Biopreservative

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