Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum

Xu, Yu; Zhang, Xuan; Yan, Xiaohui; Zhang, Jialin; Wang, Liyan; Hai, Xue; Jiang, Gaopeng; Ma, Xingkong; Liu, Xuejun

doi:10.1016/j.ijbiomac.2019.05.166

Cited by 164 publications

(50 citation statements)

References 44 publications

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“…Therefore, further research was highly suggested to investigate the roles of polysaccharides of Galla Turcica in the regulation of those potential targets, such as superoxide dismutase, catalase, malondialdehyde, reduced glutathione, glutathione peroxidase, and glutathione reductase. [30][31][32] Taken together, the work determined polysaccharides as a bioactive antioxidant of Galla Turcica on both chemical and cellular models for the first time.…”

Section: Discussionmentioning

confidence: 94%

Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

Gao

Zhao

et al. 2020

Natural Product Communications

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Though natural polysaccharides commonly show antioxidant activities, the current research on the isolation of polysaccharides from Galla Turcica and their antioxidant activities still remain as an ongoing challenge. In this work, response surface analysis was employed to optimize an ultrasonic-assisted extraction method for polysaccharides of Galla Turcica. Their antioxidant and free radical scavenging activities were then evaluated using 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), total antioxidant activity, and iron ion reduction assays. Moreover, the protective effects of polysaccharides of Galla Turcica were determined on human embryonic kidney fibroblast 293 and hepatoma 7721 cells by cell proliferation assay. Overall, the key parameters of Galla Turcica polysaccharides extraction were optimized as crushing degree 100 mesh, ultrasonic time 50 min, and materials–liquid ratio 1:50. The isolated polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in vitro. It also demonstrated an effective protective effect for human cells under oxidative damage. The results firstly determined the antioxidant activities of polysaccharides from Galla Turcica, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products, or additives.

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Section: Discussionmentioning

confidence: 94%

Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

Gao

Zhao

et al. 2020

Natural Product Communications

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“…But the Mw of the polysaccharides that we obtained from R. Laevigatae fruits were less than 10 kDa, far less than the previous studies. Previous studies have reported that the large molecular weight of polysaccharides (over 10 kDa) led to poor water solubility and unfavorable for the absorption into the body, reduced the biological activities, and possibly limited the applications of polysaccharides, whereas the low molecular weight polysaccharides less than 10 kDa had relatively higher activities to human health [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

Extraction and Hypolipidemic Activity of Low Molecular Weight Polysaccharides Isolated from Rosa Laevigata Fruits

Zhang

Jin

et al. 2020

BioMed Research International

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Three novel low molecular weight polysaccharides (RLP-1a, RLP-2a, and RLP-3a) with 9004, 8761, and 7571 Da were first obtained by purifying the crude polysaccharides from the fruits of a traditional Chinese medicinal herb Rosae Laevigatae. The conditions for polysaccharides from the R. Laevigatae fruit (RLP) extraction were optimized by the response surface methodology, and the optimal conditions were as follows: extraction temperature, 93°C; extraction time, 2.8 h; water to raw material ratio, 22; extraction frequency, 3. Structural characterization showed that RLP-1a consisted of rhamnose, arabinose, xylose, glucose, and galactose with the ratio of 3.14 : 8.21 : 1 : 1.37 : 4.90, whereas RLP-2a was composed of rhamnose, mannose, glucose, and galactose with the ratio of 1.70 : 1 : 93.59 : 2.73, and RLP-3a was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with the ratio of 6.04 : 26.51 : 2.05 : 1 : 3.17 : 31.77. The NMR analyses revealed that RLP-1a, RLP-2a, and RLP-3a contained 6, 4, and 6 types of glycosidic linkages, respectively. RLP-1a and RLP-3a exhibited distinct antioxidant abilities on the superoxide anions, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and hydroxyl radicals in vitro. RLPs could decrease the serum lipid levels, elevate the serum high-density lipoprotein cholesterol levels, enhance the antioxidant enzymes levels, and upregulate of FADS2, ACOX3, and SCD-1 which involved in the lipid metabolic processes and oxidative stress in the high-fat diet-induced rats. These results suggested that RLPs ameliorated the high-fat diet- (HFD-) induced lipid metabolism disturbance in the rat liver through the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Low molecular weight polysaccharides of RLP could be served as a novel potential functional food for improving hyperlipidemia and liver oxidative stress responses.

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“…The carboxyl group was also found at 1,400 cm −1 in GYT‐1, which proved the existence of glucuronic acid. The signals at 1,244 cm −1 and 1,246 cm −1 were attributed to the S=O stretching vibration absorption peak (Xu et al, ), indicating the presence of sulfate in GYT‐1 and GYT‐3. These results were verified in Table .…”

Section: Resultsmentioning

confidence: 99%

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

Zhang

et al. 2019

Food Science & Nutrition

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The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of polysaccharides from the heads of Hypomesus olidus. The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid–liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.73 ± 0.042%. Three polysaccharide fractions, GYT‐1, GYT‐2, and GYT‐3 were purified from GYT by using DEAE‐cellulose‐52 column and Sephadex G‐100 column for anticoagulant activities. The results showed that two doses (2 and 4 mg/ml) of GYT‐1 and GYT‐3 could significantly prolong (p < .01) in partial thromboplastin time (APTT) (2.19 and 2.37 times, 2.22 and 2.44 times, respectively) and thrombin time (TT) (2.39 and 2.46 times, 2.44 and 2.80 times, respectively) compared with normal control. In particular, GYT‐3 had stronger anticoagulant activity than GYT‐1, and it was composed of arabinose, fructose, glucose, and lactose with molar ratios of 0.595:1: 2.026:0.273. However, GYT‐2 had no anticoagulant activity (p > .05). In addition, anticoagulation mechanism of polysaccharides from the heads of Hypomesus olidus (GYT‐3) was evaluated. The results showed that the anticoagulant activity of GYT‐3 was based on their binding with antithrombin AT‐III. And the inhibitory effects of GYT‐3 on factor IIa and Xa were related to the concentration of AT‐III in plasma. This study may provide a new and promising anticoagulant drug.

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Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum

Cited by 164 publications

References 44 publications

Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

Extraction and Hypolipidemic Activity of Low Molecular Weight Polysaccharides Isolated from Rosa Laevigata Fruits

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

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