Characterization, isolation and amino terminal sequencing of a rat colon carcinoma‐associated antigen

Chadéneau, Corinne; Denis, Marc G.; Grégoire, Marc; Douillard, Jean-Yves; Méflah, Khaled

doi:10.1002/ijc.2910470620

Cited by 16 publications

(9 citation statements)

References 31 publications

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“…(2,3) Rodent Tage4 was originally identified in the rat by monoclonal antibody screens to isolate tumor-specific markers of hepatocellular carcinoma (4) and colon carcinoma. (5)(6)(7)(8) Tage4 was also found to be expressed in fetal rat liver.…”

mentioning

confidence: 92%

Overexpression of Necl‐5 correlates with unfavorable prognosis in patients with lung adenocarcinoma

Nakai¹,

Maniwa²,

Tanaka³

et al. 2010

Cancer Science

116

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Nectin-like molecule-5 (Necl-5) is an immunoglobulin (Ig)-like molecule that is up-regulated in many types of cancer cells. It was shown experimentally that Necl-5 enhances cell migration, proliferation, and metastasis, but its clinical significance has not been documented. The aim of this study was to observe the expression of Necl-5 in surgically resected primary lung adenocarcinomas and to investigate its clinical significance. A total of 63 surgically resected primary pulmonary adenocarcinoma tissues were investigated by immunohistochemistry for the expression of Necl-5. The relationship between expression of Necl-5 and clinicopathological features was analyzed, and the influence of Necl-5 expression on outcomes in these patients was assessed. A strong expression of Necl-5 by cancer cells was observed in 43 of the 63 tumors. The overexpression of Necl-5 by cancer cells was significantly associated with lymph node metastasis (P = 0.0398), TNM staging (P = 0.0367), and the bronchioloalveolar carcinoma ratio of tumors (P = 0.0423). Furthermore, the disease-free survival rate in patients with positive Necl-5 overexpression was significantly lower than that in patients with negative Necl-5 overexpression (P = 0.0004). Multivariate survival analysis revealed Necl-5 expression to be an independent risk factor for an unfavorable outcome (P = 0.0294). Additionally, an analysis including only the stage I cases revealed that the diseasefree survival rate of the Necl-5-positive group was significantly lower than that of the Necl-5-negative group (P = 0.0192). These results indicate that Necl-5 plays a role in mediating tumor cell invasion and that the overexpression of Necl-5 in cancer cells has clinical significance for prognostic evaluation of patients with primary pulmonary adenocarcinoma. (Cancer Sci 2010; 101: 1326-1330 N ecl-5 ⁄ poliovirus receptor (PVR) ⁄ CD155 ⁄ Tage4 is an immunoglobulin(Ig)-like molecule with a domain structure consisting of one extracellular region with three Ig-like loops, one transmembrane region, and one cytoplasmic region.(1) Human PVR ⁄ CD155 was originally identified as the PVR in a screen of genomic DNA from cells susceptible to poliovirus infection.(2,3) Rodent Tage4 was originally identified in the rat by monoclonal antibody screens to isolate tumor-specific markers of hepatocellular carcinoma (4) and colon carcinoma.(5-8)Tage4 was also found to be expressed in fetal rat liver.PVR ⁄ CD155 was subsequently shown to be overexpressed in many human cancer cells. (9)(10)(11) The PVR ⁄ CD155 gene thus far has been found only in primates and the Tage4 gene has been found only in rodents, but these genes are likely to be derived from a common ancestral gene; (12,13) therefore, PVR ⁄ CD155 and Tage4 were renamed nectin-like molecule-5, or Necl-5. (1,14) Nectins are Ca 2+ -independent Ig-like cell-cell adhesion molecules.(1) Nectin-like molecules (Necls) have been designated as a group of Ig-like molecules with domain structures similar to, but slightly different from, those of nectin.(1)The p...

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mentioning

confidence: 92%

Overexpression of Necl‐5 correlates with unfavorable prognosis in patients with lung adenocarcinoma

Nakai¹,

Maniwa²,

Tanaka³

et al. 2010

Cancer Science

116

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“…The rodent ortholog of Necl-5 (Tage4/PVR/CD155) was identified in rats as a tumor-specific marker of hepatocellular carcinoma [78,79,80] and colon carcinoma [81, 82], while Necl-5 is known as a poliovirus receptor/CD155 mediating the entry of human poliovirus [83]. In contrast to the low-level expression of Necl-5 in most adult organs, Necl-5 is overexpressed in various types of cancer cells [80,84,85,86,87] and a wide range of transformed cell lines [19, 80, 81, 84, 88].…”

Section: Biological Properties Of Nectins and Neclsmentioning

confidence: 99%

Nectin and Nectin-Like Molecules: Biology and Pathology

Miyoshi

Takai

2007

Am J Nephrol

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Nectins and nectin-like molecules (Necls) are structurally related transmembrane proteins primarily involved in cell adhesion. Nectins and afadin, the adaptor or anchoring protein, stabilize the epithelium and endothelium and establish apical-basal polarity of epithelial cells, independently or in cooperation with other cell adhesion molecules. Necls facilitate cell–cell communication implicated in cell movement and proliferation, immune responses, and cancer cell phenotypes. Necls interact with nectins and specific ligands at cell–cell contacts, whereas Necls associate with integrin αvβ3 and growth factor receptors on the same cell surface. Besides their roles in cell adhesion, nectins regulate the activities of Rho family small G proteins which play critical roles in maintaining the apical junctions of epithelial cells through reorganization of the actin cytoskeleton. Since mice lacking the Rho GDP-dissociation inhibitor (GDI)α show massive proteinuria and degeneration of renal epithelial cells, nectins and other cell adhesion molecules may play roles in the structural and functional aspects of renal diseases. Here we summarize our knowledge of nectins and Necls and discuss cell adhesion biology in the kidney.

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“…Cells or spheroids were extracted by Nonidet P-40 (Chadeneau et al, 1991) and dialysed. Extracted CEA was assayed by a microparticulate immunoenzymologic method (Imx SELECT CEA).…”

Section: Cea Extraction and Assaymentioning

confidence: 99%

Comparative targeting of human colon-carcinoma multicell spheroids using one- and two-step (bispecific antibody) techniques

Devys

Thédrez

Gautherot³

et al. 1996

Int. J. Cancer

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In the perspective of radioimmunotherapy (RIT) of micrometastases, we compared, in multicell spheroids (MS), the uptake and retention kinetics of 125I-F(ab)'2 F6 anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb), and the affinity enhancement system (AES) using an anti-CEA/anti-DTPA-indium bispecific antibody (BsMAb) and a 125I-labeled di-DTPA-In-tyrosine-lysine bivalent hapten. We used MS of colorectal tumor cell lines expressing CEA strongly (LS 174T), weakly (HT-29) or not at all (HRT-18). Uptake and retention kinetics of 125I-F(ab)'2 F6 and 125I-BsMAb used alone gave similar results. The highest uptake values, obtained with LS 174T MS, were slightly lower with AES than with 125I-F(ab)'2 F6. However, effective retention half-lives were longer for AES than for 125I-F(ab)'2 F6 or for 111In-labeled monovalent hapten after pre-incubation of spheroids with BsMAb. Autoradiography showed the same slow and heterogeneous distribution of 125I-F(ab)'2 F6 and 125I-BsMAb. These results indicate that the 2-step technique is more favorable for RIT: uptake values were approximately the same but uptake kinetics were more rapid, and retention half-life was longer than with the one-step technique.

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Characterization, isolation and amino terminal sequencing of a rat colon carcinoma‐associated antigen

Cited by 16 publications

References 31 publications

Overexpression of Necl‐5 correlates with unfavorable prognosis in patients with lung adenocarcinoma

Overexpression of Necl‐5 correlates with unfavorable prognosis in patients with lung adenocarcinoma

Nectin and Nectin-Like Molecules: Biology and Pathology

Comparative targeting of human colon-carcinoma multicell spheroids using one- and two-step (bispecific antibody) techniques

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