Characterization, localization, and seasonal changes of the sucrose transporter FeSUT1 in the phloem of<i>Fraxinus excelsior</i>

Öner-Sieben, Soner; Rappl, Christine; Sauer, Norbert; Stadler, Ruth; Lohaus, Gertrud

doi:10.1093/jxb/erv255

Cited by 24 publications

(14 citation statements)

References 75 publications

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“…Some of these clones have been shown to be functional SUTs by expressing them in Xenopus oocytes or yeast (Deol et al, 2013; Oner-Sieben et al, 2015; Zanon et al, 2015), but their ability to regulate plant metabolism remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Cai

et al. 2017

Front. Plant Sci.

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The import of sugar from source leaves and it further accumulation in grape berries are considerably high during ripening, and this process is mediated via sucrose transporters. In this study, a grape sucrose transporter (SUT) gene, VvSUC27, located at the plasma membrane, was transferred to tobacco (Nicotiana tabacum). The transformants were more sensitive to sucrose and showed more rapid development, especially roots, when cultured on MS agar medium containing sucrose, considering that the shoot/root dry weight ratio was only half that of the control. Moreover, all transformed plants exhibited light-colored leaves throughout their development, which indicated chlorosis and an associated reduction in photosynthesis. The total sugar content in the roots and stems of transformants was higher than that in control plants. No significant difference was observed in the leaves between the transformants and control plants. The levels of growth-promoting hormones were increased, and those of stress-mediating hormones were reduced in transgenic tobacco plants. The qRT-PCR analysis revealed that the expression of VvSUC27 was 1,000 times higher than that of the autologous tobacco sucrose transporter, which suggested that the markedly increased growth rate of transformants was because of the heterogeneously expressed gene. The transgenic tobacco plants showed resistance to abiotic stresses. Strikingly, the overexpression of VvSUC27 leaded to the up regulation of most reactive oxygen species scavengers and abscisic acid-related genes that might enable transgenic plants to overcome abiotic stress. Taken together, these results revealed an important role of VvSUC27 in plant growth and response to abiotic stresses, especially in the presence of sucrose in vitro.

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Section: Introductionmentioning

confidence: 99%

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Cai

et al. 2017

Front. Plant Sci.

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“…Early structural evidence suggested that ICs and OCCs were found in the minor veins of cucurbits (Turgeon et al ., ; Schmitz et al ., ; Rennie and Turgeon, ). In some other species, for instance, Asarina barclaiana and Acanthus mollis contained TCs and ICs (Voitsekhovskaja et al ., ), while Amborella trichopoda and Fraxinus excelsior included OCCs and ICs (Fu et al ., ; Öner‐Sieben et al ., ) in the minor veins. These phenomena suggest that apoplastic and polymer‐trapping mechanisms are probably at work in individual cells of the same veins.…”

Section: Discussionmentioning

confidence: 94%

“…It has also been found that some plants, such as Alonsoa meridionalis and Amborella trichopoda , could employ both polymer‐trapping and apoplastic loading mechanisms based on the presence of ICs and ordinary companion cells (OCCs) in the minor veins (Knop et al ., ; Voitsekhovskaja et al ., ; Turgeon and Medville, ). For Fraxinus excelsior , a RFOs‐transporting species, FeSUT1 was found and localized in SEs and possibly in OCCs of minor veins (Öner‐Sieben and Lohaus, ; Öner‐Sieben et al ., ). In the same veins of Amborella, a stachyose synthase gene, AmSTS1, was expressed in ICs but not in OCCs in which the sucrose transporter (AmSUT1) was expressed (Voitsekhovskaja et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Phloem loading in cucumber: combined symplastic and apoplastic strategies

Sun

Sui

et al. 2019

The Plant Journal

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These authors contributed equally to this work. SUMMARYPhloem loading, as the first step of transporting photoassimilates from mesophyll cells to sieve element-companion cell complex, creates a driving force for long-distance nutrient transport. Three loading strategies have been proposed: passive symplastic loading, apoplastic loading and symplastic transfer followed by polymer-trapping of stachyose and raffinose. Although individual species are generally referred to as using a single phloem loading mechanism, it has been suggested that some plants may use more than one, i.e. 'mixed loading'. Here, by using a combination of electron microscopy, reverse genetics and 14 C labeling, loading strategies were studied in cucumber, a polymer-trapping loading species. The results indicate that intermediary cells (ICs), which mediate polymer-trapping, and ordinary companion cells, which mediate apoplastic loading, were mainly found in the fifth and third order veins, respectively. Accordingly, a cucumber galactinol synthase gene (CsGolS1) and a sucrose transporter gene (CsSUT2) were expressed mainly in the fifth/third and the third order veins, respectively. Immunolocalization analysis indicated that CsGolS1 was localized in companion cells (CCs) while CsSUT2 was in CCs and sieve elements (SEs). Suppressing CsGolS1 significantly decreased the stachyose level and increased sucrose content, while suppressing CsSUT2 decreased the sucrose level and increased the stachyose content in leaves. After 14 CO 2 labeling, [ 14 C]sucrose export increased and [ 14 C]stachyose export reduced from petioles in CsGolS1i plants, but [ 14 C]sucrose export decreased and [ 14 C]stachyose export increased into petioles in CsSUT2i plants. Similar results were also observed after pre-treating the CsGolS1i leaves with PCMBS (transporter inhibitor). These results demonstrate that cucumber phloem loading depends on both polymer-trapping and apoplastic loading strategies.

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“…While concerns have been raised regarding the accuracy of the different methods used to investigate sugar concentrations (see discussion in Reidel et al and Öner‐Sieben and Lohaus ), measurement errors are unlikely to explain the massive concentration difference between phloem and mesophyll observed in species like Q. robur (Öner‐Sieben and Lohaus ). It has been speculated that either the respective PD are closed and the plants under study use active apoplasmic loading, as in herbs, or these species represent a mixed loading type (Öner‐Sieben et al, ).…”

Section: Discrepancy In Classification and Potential For Mixed Loadinmentioning

confidence: 99%

Sucrose transporters and plasmodesmal regulation in passive phloem loading

Liesche

2017

JIPB

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An essential step for the distribution of carbon throughout the whole plant is the loading of sugars into the phloem in source organs. In many plants, accumulation of sugars in the sieve element-companion cell (SE-CC) complex is mediated and regulated by active processes. However, for poplar and many other tree species, a passive symplasmic mechanism of phloem loading has been proposed, characterized by symplasmic continuity along the pre-phloem pathway and the absence of active sugar accumulation in the SE-CC complex. A high overall leaf sugar concentration is thought to enable diffusion of sucrose into the phloem. In this review, we critically evaluate current evidence regarding the mechanism of passive symplasmic phloem loading, with a focus on the potential influence of active sugar transport and plasmodesmal regulation. The limited experimental data, combined with theoretical considerations, suggest that a concomitant operation of passive symplasmic and active phloem loading in the same minor vein is unlikely. However, active sugar transport could well play an important role in how passively loading plants might modulate the rate of sugar export from leaves. Insights into the operation of this mechanism has direct implications for our understanding of how these plants utilize assimilated carbon.

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Characterization, localization, and seasonal changes of the sucrose transporter FeSUT1 in the phloem ofFraxinus excelsior

Abstract: HighlightIn Fraxinus excelsior the sucrose transporter FeSUT1 is located in the phloem and involved in apoplastic sucrose loading and retrieval. The expression of FeSUT1 is higher under low sucrose conditions.

Cited by 24 publications

References 75 publications

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Phloem loading in cucumber: combined symplastic and apoplastic strategies

Sucrose transporters and plasmodesmal regulation in passive phloem loading

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