Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents

Thaler, Andrew David; Zelnio, Kevin A.; Jones, Rebecca; Carlsson, Jens; Dover, Cindy Lee Van; Schultz, Thomas F.

doi:10.1007/s12686-010-9174-9

Cited by 7 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[39]. To assess marker quality, allelic richness and divergence from Hardy-Weinberg Equilibrium (HWE) were calculated in GENEPOP (version 4.0, [63]).…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA was isolated by Chelex-Proteinase-K extraction as described in Thaler et al . [ 39 ] and extracted DNA was stored at 4°C until amplification.…”

Section: Methodsmentioning

confidence: 99%

“…Nine microsatellite markers ( Ifr040, Ifr043, Ifr052, Ifr068, Ifr078, Ifr086, Ifr093, Ifr094 , and Ifr103 ) were amplified from Manus, North Fiji, and Lau Basin samples following methods reported in Thaler et al . [ 39 ]. To assess marker quality, allelic richness and divergence from Hardy-Weinberg Equilibrium (HWE) were calculated in GENEPOP (version 4.0, [ 63 ]).…”

Section: Methodsmentioning

confidence: 99%

“…The entire Manus Basin population was then compared to North Fiji and Lau basin samples to assess the relationship between increasing spatial scales (1000 kilometres to 3500 kilometres) and genetic differentiation. Genetic markers for differentiation at these scales included partial sequences of mitochondrial cytochrome- c -oxidase subunit I, and an array of nuclear DNA microsatellite loci [ 39 ]. By comparing these two types of molecular markers, we can separate evolutionary processes, revealed by COI sequence data and dependent on mutation rates, from ecologic processes, revealed by microsatellite allele frequencies and based on the recombination of alleles with each generation [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific

et al. 2011

Self Cite

View full text Add to dashboard Cite

BackgroundDeep-sea hydrothermal vents provide patchy, ephemeral habitats for specialized communities of animals that depend on chemoautotrophic primary production. Unlike eastern Pacific hydrothermal vents, where population structure has been studied at large (thousands of kilometres) and small (hundreds of meters) spatial scales, population structure of western Pacific vents has received limited attention. This study addresses the scale at which genetic differentiation occurs among populations of a western Pacific vent-restricted gastropod, Ifremeria nautilei.ResultsWe used mitochondrial and DNA microsatellite markers to infer patterns of gene flow and population subdivision. A nested sampling strategy was employed to compare genetic diversity in discrete patches of Ifremeria nautilei separated by a few meters within a single vent field to distances as great as several thousand kilometres between back-arc basins that encompass the known range of the species. No genetic subdivisions were detected among patches, mounds, or sites within Manus Basin. Although I. nautilei from Lau and North Fiji Basins (~1000 km apart) also exhibited no evidence for genetic subdivision, these populations were genetically distinct from the Manus Basin population.ConclusionsAn unknown process that restricts contemporary gene flow isolates the Manus Basin population of Ifremeria nautilei from widespread populations that occupy the North Fiji and Lau Basins. A robust understanding of the genetic structure of hydrothermal vent populations at multiple spatial scales defines natural conservation units and can help minimize loss of genetic diversity in situations where human activities are proposed and managed.

show abstract

“…[39]. To assess marker quality, allelic richness and divergence from Hardy-Weinberg Equilibrium (HWE) were calculated in GENEPOP (version 4.0, [63]).…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA was isolated by Chelex-Proteinase-K extraction as described in Thaler et al . [ 39 ] and extracted DNA was stored at 4°C until amplification.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Nautilus Solwara Project appears to be heavily invested in developing an implementation plan based on ecologically sound ‘best practices’ for sustainable recovery of minerals, and they have funded genetic studies aimed at assessing the potentially unique nature of the Manus Basin fauna (e.g. Thaler et al. 2010).…”

Section: The Past Present and Future Of Ventsmentioning

confidence: 99%

Genetic diversity and connectivity of deep-sea hydrothermal vent metapopulations

2010

View full text Add to dashboard Cite

Deep-sea hydrothermal vents provide ephemeral habitats for animal communities that depend on chemosynthetic primary production. Sporadic volcanic and tectonic events destroy local vent fields and create new ones. Ongoing dispersal and cycles of extirpation and colonization affect the levels and distribution of genetic diversity in vent metapopulations. Several species exhibit evidence for stepping-stone dispersal along relatively linear, oceanic, ridge axes. Other species exhibit very high rates of gene flow, although natural barriers associated with variation in depth, deep-ocean currents, and lateral offsets of ridge axes often subdivide populations. Various degrees of impedance to dispersal across such boundaries are products of species-specific life histories and behaviours. Though unrelated to the size of a species range, levels of genetic diversity appear to correspond with the number of active vent localities that a species occupies within its range. Pioneer species that rapidly colonize nascent vents tend to be less subdivided and more diverse genetically than species that are slow to establish colonies at vents. Understanding the diversity and connectivity of vent metapopulations provides essential information for designing deep-sea preserves in regions that are under consideration for submarine mining of precious metals.

show abstract

Application of Biological Studies to Governance and Management of the Deep Sea

Dover

Arnaud‐Haond

Clark

et al. 2016

Biological Sampling in the Deep Sea

View full text Add to dashboard Cite

Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents

Cited by 7 publications

References 18 publications

The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific

The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific

Genetic diversity and connectivity of deep-sea hydrothermal vent metapopulations

Application of Biological Studies to Governance and Management of the Deep Sea

Contact Info

Product

Resources

About