Characterization of a broad-spectrum endolysin rLysJNwz and its utility against Salmonella in foods

Shen, Kaisheng; Shu, Mei; Zhong, Chunjiu; Zhao, Yuan‐Yang; Bao, Shiwei; Pan, Hong; Wang, Shuchao; Wu, Guoping

doi:10.1007/s00253-023-12500-9

Cited by 8 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They can be employed for targeted bacterial detection due to their specificity towards host bacteria (Wang et al, 2017;He et al, 2018;Liu et al, 2022). Additionally, biotechnological advancements have enabled the expression of bacteriophage lytic enzymes and Frontiers in Microbiology 07 frontiersin.org endolysins, expanding the spectrum of bacteriophage lysis and facilitating the development of novel antibacterial treatments (Schuch et al, 2019;Deng et al, 2023;Shen K. et al, 2023). Bacteriophage YSP2, a highly lytic bacteriophage previously isolated in our research (Tie et al, 2018), demonstrates a specific lysis spectrum targeting Salmonella.…”

Section: Discussionmentioning

confidence: 83%

“…They can be employed for targeted bacterial detection due to their specificity towards host bacteria ( Wang et al, 2017 ; He et al, 2018 ; Liu et al, 2022 ). Additionally, biotechnological advancements have enabled the expression of bacteriophage lytic enzymes and endolysins, expanding the spectrum of bacteriophage lysis and facilitating the development of novel antibacterial treatments ( Schuch et al, 2019 ; Deng et al, 2023 ; Shen K. et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Binding activity and specificity of tail fiber protein 35Q for Salmonella pullorum

Deng,

Feng,

Shi

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Salmonella, a prevalent pathogen with significant implications for the poultry industry and food safety, presents a global public health concern. The rise in antibiotic resistance has exacerbated the challenge of prevention. Accurate and sensitive detection methods are essential in combating Salmonella infections. Bacteriophages, viruses capable of targeting and destroying bacteria, leverage their host specificity for accurate microbial detection. Notably, the tail fiber protein of bacteriophages plays a crucial role in recognizing specific hosts, making it a valuable tool for targeted microbial detection. This study focused on the tail fiber protein 35Q of Salmonella pullorum (SP) bacteriophage YSP2, identified through protein sequencing and genome analysis. Bioinformatics analysis revealed similarities between 35Q and other Salmonella bacteriophage tail fiber proteins. The protein was successfully expressed and purified using an Escherichia coli expression system, and its binding activity and specificity were confirmed. ELISA assays and adsorption experiments demonstrated that 35Q interacts with the outer membrane protein (OMP) receptor on bacterial surfaces. This investigation provides valuable insights for targeted Salmonella detection, informs the development of specific therapeutics, and enhances our understanding of the interaction between Salmonella bacteriophages and their hosts.

show abstract

Section: Discussionmentioning

confidence: 83%

“…They can be employed for targeted bacterial detection due to their specificity towards host bacteria ( Wang et al, 2017 ; He et al, 2018 ; Liu et al, 2022 ). Additionally, biotechnological advancements have enabled the expression of bacteriophage lytic enzymes and endolysins, expanding the spectrum of bacteriophage lysis and facilitating the development of novel antibacterial treatments ( Schuch et al, 2019 ; Deng et al, 2023 ; Shen K. et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%