Pakistolides A and B, novel dimeric b-(glucosyloxy)benzoates were isolated from Berchemia pakistanica and assigned structures 1 and 2 on the basis of extensive NMR studies. In addition, the known compounds 7,5'-dimethoxy-3,5,2'-trihydroxyflavone ( 3,5-dihydroxy-2-(2-hydroxy-5-methoxyphenyl)-7-methoxy-4H-1-benzopyran-4-one), 4',5-dihydroxy-3,6,7-trimethoxyflavone ( 5-hydroxy-2-(4-hydroxyphenyl)-3,6,7-trimethoxy-4H-1-benzopyran-4-one), 5,6-dihydroxy-4,7-dimethoxy-2-methylanthracene-9,10-dione, and 1,3,4-trihydroxy-6,7,8-trimethoxy-2-methylanthracene-9,10-dione were reported for the first time from the genus Berchemia. Both 1 and 2 showed significant a-glucosidase and lipoxygenase inhibitory activities, while 2 also showed antioxidant potential.