Characterization of a New Bacteriocin, Carocin D, from
            <i>Pectobacterium carotovorum</i>
            subsp.
            <i>carotovorum</i>
            Pcc21

Roh, Eunjung; Park, Tae-Ho; Kim, Myungil; Lee, Seungdon; Ryu, Sangryeol; Oh, Chang‐Sik; Rhee, Sangkee; Kim, Doo-Ho; Park, Beom-Seok; Heu, Sunggi

doi:10.1128/aem.03103-09

Cited by 58 publications

(64 citation statements)

References 21 publications

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“…(which causes the greening and blight of rice and banana; Pham et al , ; Ghequire et al , ); Ralstonia solanacearum (which causes potato brown rot and bacterial wilt of tomato; Huerta et al , ) and Pectobacterium and Dickeya spp. (which cause potato soft rot and blackleg; Roh et al , ; Chan et al , ; Grinter et al , ; Czajkowski et al , ) .…”

Section: Discussionmentioning

confidence: 99%

Engineering bacteriocin‐mediated resistance against the plant pathogen Pseudomonas syringae

Rooney

Grinter

Correia

et al. 2019

Plant Biotechnology Journal

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Summary The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against these pathogens but have limited success and may have adverse environmental impacts. Consequently, there is a pressing need to develop alternative strategies to combat bacterial disease in crops. One such strategy involves using narrow‐spectrum protein antibiotics (so‐called bacteriocins), which diverse bacteria use to compete against closely related species. Here, we demonstrate that one bacteriocin, putidacin L1 (PL1), can be expressed in an active form at high levels in Arabidopsis and in Nicotiana benthamiana in planta to provide effective resistance against diverse pathovars of Ps. Furthermore, we find that Ps strains that mutate to acquire tolerance to PL1 lose their O‐antigen, exhibit reduced motility and still cannot induce disease symptoms in PL1‐transgenic Arabidopsis. Our results provide proof‐of‐principle that the transgene‐mediated expression of a bacteriocin in planta can provide effective disease resistance to bacterial pathogens. Thus, the expression of bacteriocins in crops might offer an effective strategy for managing bacterial disease, in the same way that the genetic modification of crops to express insecticidal proteins has proven to be an extremely successful strategy for pest management. Crucially, nearly all genera of bacteria, including many plant pathogenic species, produce bacteriocins, providing an extensive source of these antimicrobial agents.

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Section: Discussionmentioning

confidence: 99%

Engineering bacteriocin‐mediated resistance against the plant pathogen Pseudomonas syringae

Rooney

Grinter

Correia

et al. 2019

Plant Biotechnology Journal

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“…(greening and blight of rice and banana) (33,34); Ralstonia solanacearum (potato brown rot and bacterial wilt of tomato) (35) and Pectobacterium and Dickeya spp. (potato soft rot and black leg) (36)(37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

Engineering bacteriocin-mediated resistance against plant pathogenic bacteria in plants

Rooney

Grinter

Correia

et al. 2019

Preprint

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Pseudomonas syringae (Ps) and related plant pathogenic bacteria are responsible for losses in diverse crops such as tomato, kiwifruit, pepper, olive and soybean. Current solutions, involving the use of chemicals and the introduction of resistance genes, have enjoyed only limited success and may have adverse environmental impacts. Consequently, there is a pressing need to develop alternative technologies to address the problem of bacterial disease in crops. An alternative strategy is to utilise the narrow spectrum protein antibiotics

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“…Recently, a novel bacteriocin, carocin D, was isolated from P. carotovorum subsp. carotovorum strain Pcc21 to develop a biocontrol agent to control this phytopathogen (9). Furthermore, to increase this antibacterial activity, an additional biocontrol agent such as bacteriophage treatment is needed.…”

mentioning

confidence: 99%

Complete Genome Sequence of Phytopathogenic Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP1

Lee

Shin

et al. 2012

J Virol

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c Pectobacterium carotovorum subsp. carotovorum is a phytopathogen causing soft rot disease on diverse plant species. To control this plant pathogen, P. carotovorum subsp. carotovorum-targeting bacteriophage PP1 was isolated and its genome was completely sequenced to develop a novel biocontrol agent. Interestingly, the 44,400-bp genome sequence does not encode any gene involved in the formation of lysogen, suggesting that this phage may be very useful as a biocontrol agent because it does not make lysogen after host infection. This is the first report on the complete genome sequence of the P. carotovorum subsp. carotovorum-targeting bacteriophage, and it will enhance our understanding of the interaction between phytopathogens and their targeting bacteriophages.

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Characterization of a New Bacteriocin, Carocin D, from Pectobacterium carotovorum subsp. carotovorum Pcc21

Cited by 58 publications

References 21 publications

Engineering bacteriocin‐mediated resistance against the plant pathogen Pseudomonas syringae

Engineering bacteriocin‐mediated resistance against the plant pathogen Pseudomonas syringae

Engineering bacteriocin-mediated resistance against plant pathogenic bacteria in plants

Complete Genome Sequence of Phytopathogenic Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP1

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