Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation

Expression of a functional inducible nitric oxide synthase in hairy cell leukaemia and ESKOL cell line

“…Among them, the ESKOL cell line has been extensively characterized and has been widely used as a model for oncogene regulation and late B cell differentiation. 22 This cell line, obtained through the courtesy of Dr C Billard (U400 INSERM, Créteil, France) was routinely maintained in culture in RPMI-1640 medium supplemented with fresh glutamine 2 mM, sodium pyruvate 1 mM, penicillin 100 IU/ml, streptomycin 100 g/ml and with 5% FCS (Myoclone super plus; Gibco, Paisley, UK) in an atmosphere of 5% CO 2 in air. The plasmacytoma cell lines RPMI-8226 and U266, obtained from the ATCC (Rockville, MD, USA) were kept in culture in the same conditions.…”

Absence of BRAF-V600E in the human cell lines BONNA-12, ESKOL, HAIR-M, and HC-1 questions their origin from hairy cell leukemia

“…Taken together, these results cast doubts on the HCL origin of the BONNA-12, ESKOL, HAIR-M, and HC-1 cell lines. Doubts were also reinforced by the morphologic appearance of these cell lines (similar to lymphoblastoid cells), their EBVpositivity (except for HAIR-M), [7][8][9][10] and their lack of the typical HCL immunophenotype (coexpression of annexin-1/CD25/CD11c/ CD103; not shown). Similarly, ESKOL and HC-1 cells xenotransplanted in severely immunodeficient NSG mice showed the morphology of diffuse large B-cell lymphoma and were annexin-1 negative (Figure 1).…”

“…Moreover, no animal models of HCL are available. 3 To overcome these problems, cell lines have been established from HCL patients [7][8][9][10] and used for functional studies. However, there is no definitive evidence that they are of authentic HCL origin.…”

RhoGTPases and p53 Are Involved in the Morphological Appearance and Interferon-α Response of Hairy Cells

“…In this study, two hairy cell lines derived from patients with HCL responsive to IFN-␣ were used, HCLL-7876 19 and Eskol. 20 Two experiments were performed on primary cells from one patient to confirm our observations. Our results demonstrate that RhoGTPases contribute to their cytoskeletal defects.…”

“…Their characterization has been reported in the context of other studies. 19,20 Fresh hairy cells were collected after written informed consent was given, in accordance with the rules and tenets of the revised Helsinki protocol, and cells were isolated as previously described. 21,22 All cells were maintained in RPMI 1640 supplemented with glutamine, antibiotics (Gibco BRL, Grand Island, NY), and 10% fetal calf serum (Globepharm, Surrey, UK).…”