Characterization of a new densovirus infecting the green peach aphid Myzus persicae

Munster, Manuela van; Dullemans, A.M.; Verbeek, M.; Heuvel, J.F.J.M. van den; Reinbold, Catherine; Brault, Véronique; Clérivet, Alain; Wilk, F. van der

doi:10.1016/s0022-2011(03)00013-2

Cited by 50 publications

(64 citation statements)

References 27 publications

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“…AdDNV displays split VP ORFs, and its two largest structural proteins have different extensions to which no roles have yet been assigned. PfDNV (33), PcDNV (25), BgDNV (9,17), and MpDNV (31,32), which all have a split VP ORF, with the smaller ORF encoding the PLA2 motif, probably have similar expression strategies (27). For PfDNV, a large number of donor and acceptor splicing sites have also been identified in the VP ORFs.…”

Section: Resultsmentioning

confidence: 99%

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

Liu

Jousset

et al. 2011

J Virol

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Insect parvoviruses (densoviruses) belong to the Densovirinae subfamily of the Parvoviridae and are small, isometric, nonenveloped viruses (diameter, ϳ25 nm) that contain a linear single-stranded DNA of 4 to 6 kb (2, 3, 27). These viruses can be subdivided into two large groups, those with ambisense genomes and those with monosense genomes. Like vertebrate parvoviruses, all densoviruses have a genomic DNA with hairpins at both ends, often (but not necessarily for all genera) as inverted terminal repeats (ITRs). All densoviruses with ambisense genomes package both complementary strands in equimolecular ratios as single strands in separate capsids (27). The nonstructural (NS) gene cassette is found in the 5Ј half of one genome strand, and the structural protein (VP) gene cassette is found in the 5Ј half of the complementary strand. By convention, the genome is oriented so that the NS cassette is found in the left half. Expression strategies of densoviruses often involve (alternative) splicing and leaky scanning translation mechanisms (28). So far, the near-atomic structures of three densoviruses, Penaeus stylirostris densovirus (PstDNV), Bombyx mori densovirus 1 (BmDNV-1), and Galleria mellonella densovirus (GmDNV), have been solved (10,11,21). The capsid of densoviruses consists of 60 subunits (Tϭ1) of identical proteins that may contain N-terminal extensions not involved in capsid formation but that confer additional functions to the capsid. One of these functions is a phospholipase A2 (PLA2) activity that is required for genome delivery during infection (34). Densoviruses are usually highly pathogenic for their natural hosts (5).The monosense densoviruses have been classified into 3 uniform genera, i.e., Iteravirus, with a 5.0-kb genome, 0.25-kb ITRs, and a PLA2 motif in VP; Brevidensovirus, with a 4.0-kb genome, no ITRs but terminal hairpins, and no PLA2 motif; and Hepanvirus, with a single member, hepatopancreatic parvovirus, with a 6.3-kb genome also lacking a PLA2 motif and ITRs but with 0.2-kb terminal hairpins (23,27). In contrast, the ambisense densoviruses have been classified into one uniform genus, Densovirus, with a 6-kb genome and 0.55-kb ITRs, and a second genus, Pefudensovirus, with only Periplaneta fuliginosa densovirus (PfDNV) as a member, with a 5.5-kb genome, 0.2-kb ITRs, and a split VP gene cassette (2, 26). Ribosome frameshifts have been proposed to connect its VP open reading frames (ORFs) (33). So far, all ambisense densoviruses have an N-terminal PLA2 motif in their largest VP. Some sequenced ambisense densoviruses, e.g., Myzus persicae densovirus (MpDNV) (32), Blattella germanica densovirus (BgDNV) (18), and Planococcus citri densovirus (PcDNV) (25), are as yet unclassified. The ambisense virus Culex pipiens densovirus (CpDNV) has a different genome organization for both the NS

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Section: Resultsmentioning

confidence: 99%

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

Liu

Jousset

et al. 2011

J Virol

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“…In addition, all adult aphids collected as first instar nymphs, while being delivered by their mothers and grown on naive plants, were also infected with MeV-1, suggesting that the virus is transmitted vertically and likely transovarially. Vertical transmission from mother to nymphs has been demonstrated for DplDNV in the rosy apple aphid, and for MpDNV in Myzus persicae (Ryabov et al, 2009;van Munster et al, 2003a). However, the vertical transmission in both cases did not seem to be absolute as not all the progeny of virus-infected females were infected with the virus.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, host factors required for MeV-1 replication are missing in tomato. Most aphid viruses reported to date are detected in the aphid saliva or plant host tissues (Ban et al, 2007;Ryabov et al, 2009;van Munster et al, 2003a). Although viral multiplication in plant tissues has not been investigated for all these viruses, aphid viruses do not seem to multiply in the plants (Ryabov et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…These include two DNA viruses, Myzus persicae densovirus (MpDNV) (van Munster et al, 2003b) and Dysaphis plantaginea densovirus (DplDNV) (Ryabov et al, 2009), and five RNA viruses, aphid lethal paralysis virus (ALPV) (van Munster et al, 2002), Rhopalosiphum padi virus (Moon et al, 1998), Acyrthosiphon pisum virus (van der Wilk et al, 1997), Brevicoryne brassicae virus (Ryabov, 2007) and rosy apple aphid virus (RAAV) (Ryabov et al, 2009). While these aphid DNA viruses are around 5 kb in size the RNA viruses are larger, around 10 kb in size.…”

Section: Introductionmentioning

confidence: 99%

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A novel virus from Macrosiphum euphorbiae with similarities to members of the family Flaviviridae

Teixeira

Sela

et al. 2016

Journal of General Virology

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A virus with a large genome was identified in the transcriptome of the potato aphid (Macrosiphum euphorbiae) and was named Macrosiphum euphorbiae virus 1 (MeV-1). The MeV-1 genome is 22 780 nt in size, including 39 and 59 non-coding regions, with a single large ORF encoding a putative polyprotein of 7333 aa. The C-terminal region of the predicted MeV-1 polyprotein contained sequences with similarities to helicase, methyltransferase and RNA-dependent RNA polymerase (RdRp) motifs, while the N-terminal region lacked any motifs including structural proteins. Phylogenetic analysis of the helicase placed MeV-1 close to pestiviruses, while the RdRp region placed it close to pestiviruses and flaviviruses, suggesting MeV-1 has a positive-polarity ssRNA genome and is a member of the family Flaviviridae. Since the MeV-1 genome is predicted to contain a methyltransferase, a gene present typically in flaviviruses but not pestiviruses, MeV-1 is likely a member of the genus Flavivirus. MeV-1 was present in nymphal and adult stages of the aphid, aphid saliva and plant tissues fed upon by aphids. However, the virus was unable to multiply and spread in tomato plants. In addition, dsRNA, the replication intermediate of RNA viruses, was isolated from virus-infected M. euphorbiae and not from tomato plants infested with the aphid. Furthermore, nymphs laid without exposure to infected plants harboured the virus, indicating that MeV-1 is an aphid-infecting virus likely transmitted transovarially. The virus was present in M. euphorbiae populations from Europe but not from North America and was absent in all other aphid species tested.

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“…However, in the past decade, the development of high-throughput next-generation sequencing (NGS) technologies and bioinformatics applications has provided new opportunities for discovering viruses in many organisms, including humans (4-7), arthropods , and plants (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45). In addition to transcriptome sequencing (RNA-seq) (15,18,28), deep sequencing of small RNAs (sRNAs) and the subsequent assembly of the sRNAs have been proven to be promising approaches for the discovery of both RNA and DNA viruses in plant and insect hosts (9,41,42,(46)(47)(48)(49)(50).…”

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confidence: 99%

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

Nouri

Salem

Nigg

et al. 2016

J Virol

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The Asian citrus psyllid, Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB and D. citri represent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, and D. citri viruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population of D. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the virus-like sequences of D. citri are diverse. We identified novel viral sequences belonging to the picornavirus superfamily, the Reoviridae, Parvoviridae, and Bunyaviridae families, and an unclassified positive-sense single-stranded RNA virus. Moreover, a Wolbachia prophage-related sequence was identified. This is the first comprehensive survey to assess the viral community from worldwide populations of an agricultural insect pest. Our results provide valuable information on new putative viruses, some of which may have the potential to be used as biocontrol agents. IMPORTANCEInsects have the most species of all animals, and are hosts to, and vectors of, a great variety of known and unknown viruses. Some of these most likely have the potential to be important fundamental and/or practical resources. In this study, we used highthroughput next-generation sequencing (NGS) technology and bioinformatics analysis to identify putative viruses associated with Diaphorina citri, the Asian citrus psyllid. D. citri is the vector of the bacterium causing Huanglongbing (HLB), currently the most serious threat to citrus worldwide. Here, we report several novel viral sequences associated with D. citri. Viruses are the most abundant microbes on our planet (1) and are found in all types of organisms. Insects are the largest and most diverse taxonomic class among animals, representing perhaps half of known animals, with more than one million species recognized worldwide (2). Insects are known to be hosts to viruses belonging to various viral taxa, including the Baculoviridae, Parvoviridae, Flaviviridae, Ascoviridae, Togaviridae, Bunyaviridae, and Rhabdoviridae (3). However, the number of currently described viral species infecting insects is relatively low compared to the number of viruses that have been discovered among prokaryotes, plants, and vertebrates. Furthermore, most of the insect viruses described to date have been discovered because of their pathogenic effects on their insect hosts or because they are pathogens of humans, other vertebrates, or economically important plants. Traditional viral detection methods that require prior knowledge of genome sequences may not be suitable for the discovery of new viruses and, in particular, viruses with a high level of genetic diversity. However, in the past decade, the development of high-throughput next-generation sequencing (NGS) technologies and bioinformatics applications has provided ne...

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Characterization of a new densovirus infecting the green peach aphid Myzus persicae

Cited by 50 publications

References 27 publications

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

A novel virus from Macrosiphum euphorbiae with similarities to members of the family Flaviviridae

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

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