Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.)

Yu, Cheng-Yu; Guo, Yingfen; Ge, Juan; YuMei, Hu; Dong, Jun-Gang; Zhou, Dong

doi:10.1007/s10681-015-1514-0

Cited by 16 publications

(40 citation statements)

References 17 publications

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“…Except for excessively vacuolated tapetal cells at the microsporocyte stage (data not shown), as shown in our previous study (Yu et al, 2015), the main differences included delayed degradation of the tetrad wall, vacuolization of uni-nucleate microspores, later deposition of exine, and absence of nexine in SP2S. The microsporocytes of both SP2F and SP2S had larger nuclei (Figures 2A,B) but primary cell wall of the latter was undulated and some large vacuoles formed in the cytoplasm (Figure 2B).…”

Section: Resultssupporting

confidence: 85%

TGMS in Rapeseed (Brassica napus) Resulted in Aberrant Transcriptional Regulation, Asynchronous Microsporocyte Meiosis, Defective Tapetum, and Fused Sexine

Liu

et al. 2017

Front. Plant Sci.

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The thermo-sensitive genic male sterility (TGMS) line SP2S is a spontaneous rapeseed mutation with several traits that are favorable for the production of two-line hybrids. To uncover the key cellular events and genetic regulation associated with TGMS expression, a combined study using cytological observation, transcriptome profiling, and gene expression analysis was conducted for SP2S and its near-isogenic line SP2F grown under warm conditions. Asynchronous microsporocyte meiosis and abnormal tapetal plastids and elaioplasts were demonstrated in the anther of SP2S. The tetrad microspore did not undergo mitosis before the cytoplasm degenerated. Delayed degradation of the tetrad wall, which led to tetrad microspore aggregation, resulted in postponement of sexine (outer layer of pollen exine) formation and sexine fusion in the tetrad. The nexine (foot layer of exine) was also absent. The delay of tetrad wall degradation and abnormality of the exine structure suggested that the defective tapetum lost important functions. Based on transcriptomic comparisons between young flower buds of SP2S and SP2F plants, a total of 465 differentially expressed transcripts (DETs) were identified, including 303 up-regulated DETs and 162 down-regulated DETs in SP2S. Several genes encoding small RNA degrading nuclease 2, small RNA 2′-O-methyltransferase, thioredoxin reductase 2, regulatory subunit A alpha isoform of serine/threonine-protein phosphatase 2A, glycine rich protein 1A, transcription factor bHLH25, leucine-rich repeat receptor kinase At3g14840 like, and fasciclin-like arabinogalactan proteins FLA19 and FLA20 were greatly depressed in SP2S. Interestingly, a POLLENLESS3-LIKE 2 gene encoding the Arabidopsis MS5 homologous protein, which is necessary for microsporocyte meiosis, was down-regulated in SP2S. Other genes that were up-regulated in SP2S encoded glucanase A6, ethylene-responsive transcription factor 1A-like, pollen-specific SF3, stress-associated endoplasmic reticulum protein 2, WRKY transcription factors and pentatricopeptide repeat (PPR) protein At1g07590. The tapetum-development-related genes, including BnEMS1, BnDYT1, and BnAMS, were slightly up-regulated in 3-mm-long flower buds or their anthers, and their downstream genes, BnMS1 and BnMYB80, which affect callose dissolution and exine formation, were greatly up-regulated in SP2S. This aberrant genetic regulation corresponded well with the cytological abnormalities. The results suggested that expression of TGMS associates with complex transcriptional regulation.

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Section: Resultssupporting

confidence: 85%

TGMS in Rapeseed (Brassica napus) Resulted in Aberrant Transcriptional Regulation, Asynchronous Microsporocyte Meiosis, Defective Tapetum, and Fused Sexine

Liu

et al. 2017

Front. Plant Sci.

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“…) and SP2S (Yu et al. ). In addition, it could be also used in ‘two‐line hybrid system’, one male sterile line which can be multiplied in summer rape areas such as Qinghai, Gansu in China, and one male parent.…”

Section: Discussionmentioning

confidence: 99%

Cytological observation of anther structure and genetic investigation of a new type of cytoplasmic male sterile 0A193‐CMS in Brassica rapa L.

et al. 2016

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Cytoplasmic male sterility (CMS), a maternally transmitted failure in pollen formation, is an effective pollination control system in hybrid rapeseed (Brassica napus) breeding. However, CMS is not widely used in the related oilseed species Brassica rapa. In the past years, several male sterile plants have been isolated from the B. rapa landrace ‘0A193’, collected in Shaanxi, China, in 2011. It is noteworthy that the fertility expression of 0A193‐CMS was affected by temperature. In contrast to pol CMS, fertility tests with 18 B. rapa and 9 B. napus accessions suggest that a different system of maintaining and restoring is responsible for the observed phenotype. Further on, genetic investigation evidenced that fertility of 0A193‐CMS is controlled by both cytoplasmic and one pair of nuclear recessive genes. Interestingly, plants of the 0A193‐CMS type possess a highly specific fragment of the mitochondrial gene orf222, a crucial regulator of male sterility in nap CMS. Our study broadens the CMS resources in B. rapa and provides a highly applicable alternative to pol CMS and ogu CMS for hybrid breeding production.

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“…The most striking structural differences between the fertile and male-sterile plants were observed at microsporocyte stage. Some huge vacuoles appeared in the tapetal cells in SP2S and then premature tapetum degenerated (Yu et al 2015). SP2S featured no adverse effect on F 1 hybrids and it is a promising system for two-line hybrid rapeseed production (Yu et al 2015).…”

mentioning

confidence: 98%

“…Treatment under cool conditions (temperature < 15°C) can revert the fertility of SP2S, and this trait followed an inheritance pattern indicative of two recessive genes (Yu et al. ). The most striking structural differences between the fertile and male‐sterile plants were observed at microsporocyte stage.…”

mentioning

confidence: 99%

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Proteomic alteration of a thermo‐sensitive male sterility SP2S in rapeseed (Brassica napus) in response to mild temperature stress

Zhang

Liu

et al. 2016

Plant Breeding

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We conducted a proteomic comparison between thermo-sensitive genic male sterility (TGMS) SP2S and its near-isogenic line SP2F grown at 22°C. The proteomes at microsporocyte meiosis and uninucleate microspore stages were profiled using 2-dimensional gel electrophoresis. Twenty-five well-reproducible spots (10 spots at microsporocyte meiosis stage and 15 spots at uninucleate microspore stage) containing 28 proteins were successfully identified by MALDI-TOF/TOF mass spectrometry. An elongation factor EF-2 at microsporocyte stage and 4 proteins (aconitate hydratase, triosephosphate isomerase, serine/arginine-rich mRNA splicing factor and glutathione S-transferase) at uninucleate microspore stage accumulated in SP2S, but more proteins were lost or reduced, including those associated with amino acid metabolism, photosynthesis, synthesis and degradation of protein, lipid metabolism, cytoskeleton, RNA modification, oxidoreductase and defence response. The dramatic decrease of tubulin, actin and Translationally controlled tumour protein (TCTP) crucial for microtubule and cell division and three enzymes for amino acid metabolite at early stage indicated a serious defect on the cytokinesis. They were important clues for us to search the TGMS genes and its interacting genes.

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Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.)

Cited by 16 publications

References 17 publications

TGMS in Rapeseed (Brassica napus) Resulted in Aberrant Transcriptional Regulation, Asynchronous Microsporocyte Meiosis, Defective Tapetum, and Fused Sexine

TGMS in Rapeseed (Brassica napus) Resulted in Aberrant Transcriptional Regulation, Asynchronous Microsporocyte Meiosis, Defective Tapetum, and Fused Sexine

Cytological observation of anther structure and genetic investigation of a new type of cytoplasmic male sterile 0A193‐CMS in Brassica rapa L.

Proteomic alteration of a thermo‐sensitive male sterility SP2S in rapeseed (Brassica napus) in response to mild temperature stress

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