Characterization of a normal, tissular antigen extracted from human colonic tumors

Kleist, S. von; King, M.; Burtin, P

doi:10.1016/0019-2791(74)90203-1

Cited by 36 publications

(12 citation statements)

References 10 publications

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“…Indeed, Lipkin and co-workers have described changes in the nucleic acid metabolism and proliferative capacity of the colonic epithelium in DMH treated mice (Thurnherr et al, 1973;Deschner, 1974) which are similar to those found in the human colonic mucosa adjacent to carcinoma and neoplastic polyps (Immondi, Balis and Lipkin, 1969;Deschner and Lipkin, 1970;Troncale, Hertz and Lipkin, 1971) and in familial polyposis (Lipkin, 1974). These changes may reflect a loss of suppressor genes and a regression of the cell to a more embryonic state, a hypothesis supported in the demonstration of embryonic specific antigens in malignant tumours (Gold and Freedman, 1965;von Kleist and Burtin, 1969;Stonehill and Bendich, 1970;von Kleist, 1971;Bordes, Michiels and Martin, 1973).…”

Section: Discussionmentioning

confidence: 71%

Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study

Filipe¹

1975

Br J Cancer

178

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Summary.-Our previous studies, in specimens of large intestine resected for carcinoma, have shown abnormal patterns of mucous secretion in areas of apparently " normal " mucosa, where goblet cells produce mainly sialomucins as compared with the true normal colonic mucosa in which sulphomucins predominate. In the present work, large bowel cancer was induced in rats by the administration of 1,2-dimethylhydrazine-2HCl (DMH). We attempted to study the sequential histological and secretory abnormalities which developed in the colonic epithelium during carcinogenesis, and to correlate these changes with those described above in the human. The microscopical and histological lesions observed in the colonic mucosa of DMH treated rats confirmed the findings of other authors and resembled the human colorectal cancer. The earliest changes detected were small foci of hyperplasia accompanied from the 6th week onwards by several foci of dysplasia. Carcinoma in situ appeared at the 15th week and finally invasive carcinoma developed from the 19th week onwards. Changes in the type of mucous secretion, with predominance of sialomucins, were observed in the majority of the areas showing mild to moderate dysplasia whilst the surrounding normal epithelium produced sulphated material. Mucous depletion was a common feature in areas of severe dysplasia and carcinoma.These findings correlated well with the similar variations in the mucin composition observed in human colonic mucosa in carcinoma and further supported our previous hypothesis that mucin changes characterized by an increase in sialomucins might reflect early malignant transformation. If this hypothesis proved to be correct, the use of a simple method for the identification of mucins in large bowel biopsies would be of great help in detecting early malignancy.

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Section: Discussionmentioning

confidence: 71%

Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study

Filipe¹

1975

Br J Cancer

178

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“…Minor contaminants were albumin and gamma globulins since rabbits immunized with tumorous colonic mucosa extract developed antibodies against these serum constituents. The main beta protein seems to be identical with the CEA described and characterized by Gold and Freedman (1965a, b), Krupey et al (1968) and von Kleist and Burtin (1969a). In normal colonic mucosa extract the alpha protein was detected together with another unidentified fraction with an immunoelectrophoretic behavior similar to that of seromucoid as major constituents.…”

Section: Immunological Characterization Of Cea and Of The Cea Antisermentioning

confidence: 70%

Carcinoembryonic antigen (CEA) in gastrointestinal and extragastrointestinal tumors and its relationship to tumor‐cell differentiation

Denk

Tappeiner

Eckerstorfer

et al. 1972

Intl Journal of Cancer

165

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“…CEA was prepared by perchloric acid extraction of a liver metastasis from a human colonic cancer and a human gastric cancer, and was further purified by gel filtration on Sephadex G-200, according to the method of von Kleist and Burtin (11). SC was prepared from an ammonium sulfate precipitate of human colostrum by chromatography on Sephadex G-200 (6).…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemistry of Gastric Carcinomas and Associated Diseases: Novel Distribution of Carcinoembryonic Antigen and Secretory Component on the Surface of Gastric Cancer Cells

Nishimura

Tsutsumi

Shioda

et al. 1983

J Histochem Cytochem.

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Carcinoembryonic antigen (CEA) and secretory component (SC) were localized by peroxidase-labeled antibody immunocytochemistry in normal and abnormal human gastric mucosa. In normal epithelium, both glycoproteins were absent or only faintly present, but in intestinal metaplasia and carcinoma both were prominently present. CEA and SC on the surfaces of metaplastic epithelial cells were polarized. That is, CEA was expressed only on the microvillous surface and SC was expressed only on the basolateral surface. In gastric cancer, CEA and SC were distributed over the entire surface of the neoplastic cells.Thus, deviations from the normal differentiation and maturation of gastric epithelial cells were accompanied by abnormalities in surface expression of CEA and SC. These observations, together with compatible observations previously made in colonic neoplasia (DJ Ahnen, PK Nakane, and WR Brown, Cancer 49:2077, 1982, suggest that loss of polarity of surface membrane components is a characteristic of neoplastic epithelial cells.

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Characterization of a normal, tissular antigen extracted from human colonic tumors

Cited by 36 publications

References 10 publications

Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study

Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study

Carcinoembryonic antigen (CEA) in gastrointestinal and extragastrointestinal tumors and its relationship to tumor‐cell differentiation

Immunohistochemistry of Gastric Carcinomas and Associated Diseases: Novel Distribution of Carcinoembryonic Antigen and Secretory Component on the Surface of Gastric Cancer Cells

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