Characterization of a ToMV isolate overcoming Tm-22 resistance gene in tomato

Kuroiwa, Misato; Handa, Syoya; Gyoutoku, Yutaka; Moriyama, Miho; Neriya, Yutaro; Nishigawa, Hisashi; Natsuaki, Tomohide

doi:10.1007/s11262-022-01921-9

Cited by 7 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most destructive symptoms caused by plant viruses is systemic necrosis, which usually results in plant death. Several viruses have been reported to cause systemic necrosis in tomato plants [ 24 , 27 , 29 ], while the underlying basis of inducing such severe disease is largely unknown. The vp26 subunit of viral CP contributes to the occurrence of systemic necrosis in tomato plants infected with ToTV [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…Systemic necrosis is the most severe viral symptom, usually leading to plant death. Systemic necrosis of tomato plants has been reported in some cases of virus infections, including ToMV [24], tomato torrado virus (ToTV) [27], rehmannia mosaic virus (ReMV) [28], parietaria mottle virus (PMoV) [29], and some satellite RNAs of cucumber mosaic virus (CMV) [30][31][32]. The strain N5 of ToMV (ToMV-N5, referred to as N5 afterward) that we isolated previously from the field-grown tomato plants induces systemic necrosis in tomato plants [33,34].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, ToMV MP is an avirulence protein, which is recognized specifically by the tomato resistance gene Tm-2 2 , leading to extreme resistance in tomatoes [ 23 ]. However, such R gene-mediated resistance can be broken through mutations of viral avirulence proteins [ 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Movement Protein Mediates Systemic Necrosis in Tomato Plants with Infection of Tomato Mosaic Virus

Liao

Guo

et al. 2023

Viruses

View full text Add to dashboard Cite

The necrogenic strain N5 of tomato mosaic virus (ToMV-N5) causes systemic necrosis in tomato cultivar Hezuo903. In this work, we mapped the viral determinant responsible for the induction of systemic necrosis. By exchanging viral genes between N5 and a non-necrogenic strain S1, we found that movement protein (MP) was the determinant for the differential symptoms caused by both strains. Compared with S1 MP, N5 MP had an additional ability to increase virus accumulation, which was not due to its functions in viral cell-to-cell movement. Actually, N5 MP, but not S1 MP, was a weak RNA silencing suppressor, which assisted viral accumulation. Sequence alignment showed that both MPs differed by only three amino acid residues. Experiments with viruses having mutated MPs indicated that the residue isoleucine at position 170 in MP was the key site for MP to increase virus accumulation, but also was required for MP to induce systemic necrosis in virus-infected tomato plants. Collectively, the lethal necrosis caused by N5 is dependent on its MP protein that enhances virus accumulation via its RNA silencing suppressor activity, probably leading to systemic necrosis responses in tomato plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Movement Protein Mediates Systemic Necrosis in Tomato Plants with Infection of Tomato Mosaic Virus

Liao

Guo

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

“…In addition, mutants with a single amino acid addition in a comovirus helicase, capable of inducing HR-type PCD upon transient expression in N. benthamiana , were shown to either compromise or accelerate cell death [ 160 ]. Recently, a single amino acid substitution in the MP of tomato mosaic virus was associated with Tm-2 2 resistance breaking and systemic necrosis [ 161 ]. Whether these different outcomes induced by variations in the viral pathogenicity determinant really reflect a continuity in the symptoms needs to be further investigated.…”

Section: Important Advances In the Comprehension Of Hrmentioning

confidence: 99%

The Hypersensitive Response to Plant Viruses

Piau,

Schmitt-Keichinger

2023

Viruses

View full text Add to dashboard Cite

Plant proteins with domains rich in leucine repeats play important roles in detecting pathogens and triggering defense reactions, both at the cellular surface for pattern-triggered immunity and in the cell to ensure effector-triggered immunity. As intracellular parasites, viruses are mostly detected intracellularly by proteins with a nucleotide binding site and leucine-rich repeats but receptor-like kinases with leucine-rich repeats, known to localize at the cell surface, have also been involved in response to viruses. In the present review we report on the progress that has been achieved in the last decade on the role of these leucine-rich proteins in antiviral immunity, with a special focus on our current understanding of the hypersensitive response.

show abstract

“…Several tobamovirus species are known to infect tomatoes and peppers. The symptoms usually include chlorotic mottle, distorted or mosaic leaves, and small, discolored, deformed fruits; these symptoms generally affect both the quantity and quality of production. − The virus particles are produced to a high titer in susceptible host species and are very stable; thus, the symptom severity varies with virus strain and host genotype. − Seeds from tobamovirus-infected plants can easily be externally contaminated with the virus, which spreads the viruses through the international trade of pepper and tomato seeds that are unknowingly contaminated. , …”

Section: Introductionmentioning

confidence: 99%

One-Step Reverse-Transcription Recombinase-Aided Amplification CRISPR/Cas12a-Based Lateral Flow Assay for Fast Field Screening and Accurate Differentiation of Four Major Tobamoviruses Infecting Tomato and Pepper

Zhao,

Wang,

Dong

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Several tobamoviruses cause substantial economic losses to tomato and pepper crops globally, especially the pepper mild mosaic virus (PMMoV), tomato brown rugose fruit virus (ToBRFV), tomato mosaic virus (ToMV), and tomato mottle mosaic virus (ToMMV). A fast and accurate detection method is essential for virus identification. An all-in-one reaction method combining a one-step reverse-transcription recombinase-aided amplification (RT-RAA) and CRISPR/Cas12a-based lateral flow assay in one mixture was developed to rapidly screen and accurately differentiate among these four tobamoviruses for field detection in tomato and pepper plants. With a generic RT-RAA primer set and a mix of four specific crRNAs, along with a portable metal incubator and the use of a crude extraction method, this method screened for PMMoV, ToBRFV, ToMV, and ToMMV concurrently in less than 1 h, enabling field workers to take action immediately. The accurate differentiation of these four viruses could be achieved by later adding a single specific crRNA.

show abstract

Characterization of a ToMV isolate overcoming Tm-22 resistance gene in tomato

Cited by 7 publications

References 19 publications

Movement Protein Mediates Systemic Necrosis in Tomato Plants with Infection of Tomato Mosaic Virus

Movement Protein Mediates Systemic Necrosis in Tomato Plants with Infection of Tomato Mosaic Virus

The Hypersensitive Response to Plant Viruses

One-Step Reverse-Transcription Recombinase-Aided Amplification CRISPR/Cas12a-Based Lateral Flow Assay for Fast Field Screening and Accurate Differentiation of Four Major Tobamoviruses Infecting Tomato and Pepper

Contact Info

Product

Resources

About