Characterization of a unique σ54–dependent PTS operon of the lactose family in Listeria monocytogenes

Dalet, Karine; Arous, Safia; Cenatiempo, Y.; Héchard, Yann

doi:10.1016/s0300-9084(03)00134-2

Cited by 27 publications

(34 citation statements)

References 28 publications

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“…The large-scale transcriptional responses in E. coli are mediated via a limited number of global regulators (527). In enteric bacteria, global regulation of carbon metabolism involves several and transcription factors such as Crp (cyclic AMP receptor protein) (reviewed in references 76,95,96,416, and 636), Mlc (666), FruR (145,267,268,762), CsrA (carbon storage regulator) (451,742,743,752,861), and 54 (25,150,843). The activities of some of these regulators are directly affected by the PTS.…”

Section: Eiia Glc Is the Central Processing Unit Of Carbon Metabolismmentioning

confidence: 99%

How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria

Deutscher

Francke

Postma

2006

Microbiol Mol Biol Rev

1,204

769

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SUMMARY The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system (PTS) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and other sugar derivatives. To carry out its catalytic function in sugar transport and phosphorylation, the PTS uses PEP as an energy source and phosphoryl donor. The phosphoryl group of PEP is usually transferred via four distinct proteins (domains) to the transported sugar bound to the respective membrane component(s) (EIIC and EIID) of the PTS. The organization of the PTS as a four-step phosphoryl transfer system, in which all P derivatives exhibit similar energy (phosphorylation occurs at histidyl or cysteyl residues), is surprising, as a single protein (or domain) coupling energy transfer and sugar phosphorylation would be sufficient for PTS function. A possible explanation for the complexity of the PTS was provided by the discovery that the PTS also carries out numerous regulatory functions. Depending on their phosphorylation state, the four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB) can phosphorylate or interact with numerous non-PTS proteins and thereby regulate their activity. In addition, in certain bacteria, one of the PTS components (HPr) is phosphorylated by ATP at a seryl residue, which increases the complexity of PTS-mediated regulation. In this review, we try to summarize the known protein phosphorylation-related regulatory functions of the PTS. As we shall see, the PTS regulation network not only controls carbohydrate uptake and metabolism but also interferes with the utilization of nitrogen and phosphorus and the virulence of certain pathogens.

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Section: Eiia Glc Is the Central Processing Unit Of Carbon Metabolismmentioning

confidence: 99%

How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria

Deutscher

Francke

Postma

2006

Microbiol Mol Biol Rev

1,204

769

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“…Among these genes, lmo1719 and lmo1720 are part of the previously described lpo operon (Dalet et al, 2003). Furthermore, there are genes for eight complete PTSs with high homology to the PTS Table 1.…”

Section: Overview Of the Pep Ptss Of L Monocytogenesmentioning

confidence: 99%

“…Deletion of lmo1721, encoding LacR (Dalet et al, 2003), abolished the transcription of lmo1719/20, identical to lpo, and lmo2683-85 (Table 5), indicating that this PRD activator is essential for the initiation of transcription from the sigma-54-specific promoters located in front of lmo1719/20 and lmo2683-85.…”

Section: Regulation Of the Operons Encoding The Pts Permeasesmentioning

confidence: 99%

“…A second LevR-homologous activator has been identified in L. monocytogenes and termed LacR, since it apparently activates sigma-54-dependent transcription of an lpo operon which encodes the EIIA and EIIB components of a PTS Lac permease (Dalet et al, 2003). Mutants with deletions in lpoO (lmo1719) and lpoA (lmo1718) exhibit, however, a similar growth rate to that of the wild-type strain when cultured in the presence of glucose/mannose or cellobiose/lactose (Dalet et al, 2003), suggesting that still other PTS permeases are involved in the transport of these carbohydrates.…”

Section: Two Members Of the Ptsmentioning

confidence: 99%

“…Earlier work (Arous et al, 2004b;Dalet et al, 2001Dalet et al, , 2003 has shown that the operons mptACD, mpoABCD and lpo (identical to the lmo0096-98, lmo0781-84 and 1719/20 operons, respectively) are transcribed by the sigma-54-dependent RNA polymerase and that the transcription of these operons is activated by ManR (Arous et al, 2004b) or LacR (Dalet et al, 2003). Both transcription activators belong to the LevR family of PRD-containing transcription activators of B. subtilis (Stülke et al, 1998;Deutscher et al, 2006).…”

Section: Regulation Of the Operons Encoding The Pts Permeasesmentioning

confidence: 99%

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The major PEP-phosphotransferase systems (PTSs) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular growth

2010

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In this report we examine the PEP-dependent phosphotransferase systems (PTSs) of Listeria monocytogenes EGD-e, especially those involved in glucose and cellobiose transport. This L. monocytogenes strain possesses in total 86 pts genes, encoding 29 complete PTSs for the transport of carbohydrates and sugar alcohols, and several single PTS components, possibly supporting transport of these compounds. By a systematic deletion analysis we identified the major PTSs involved in glucose, mannose and cellobiose transport, when L. monocytogenes grows in a defined minimal medium in the presence of these carbohydrates.

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Identification and functional characterisation of cellobiose and lactose transport systems in Lactococcus lactis IL1403

et al. 2007

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Physiological, biochemical and macroarray analyses of Lactococcus lactis IL1403 and its ccpA and bglR single and double mutants engaged in lactose and beta-glucosides catabolism were performed. The kinetic analysis indicated the presence of different transport systems for salicin and cellobiose. The control of salicin catabolism was found to be mediated by the transcriptional regulator BglR and the CcpA protein. The transcriptional analysis by macroarray technology of genes from the PEP:PTS regions showed that several genes, like ybhE, celB, ptcB and ptcA, were expressed at higher levels both in wild type cells exposed to cellobiose and in the ccpA mutant. We also demonstrated that in L. lactis IL1403 cultured on medium with cellobiose and lactose as carbon sources, after the first phase of cellobiose consumption and then co-metabolism of the two sugars, when cellobiose is exhausted the strain uses lactose as the only carbon source. These data could indicate that lactose and cellobiose are transported by a unique system-a PTS carrier induced by the presence of cellobiose, and negatively controlled by the CcpA regulator.

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Characterization of a unique σ54–dependent PTS operon of the lactose family in Listeria monocytogenes

Cited by 27 publications

References 28 publications

How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria

How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria

The major PEP-phosphotransferase systems (PTSs) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular growth

Identification and functional characterisation of cellobiose and lactose transport systems in Lactococcus lactis IL1403

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