Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata

Kumar, Dilip; Rampuria, Sakshi; Singh, Naveen Kumar; Shukla, Pawan; Kirti, Pulugurtha Bharadwaja

doi:10.1007/s11103-015-0318-x

Cited by 16 publications

(12 citation statements)

References 68 publications

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“…The qRT-PCR reaction was performed essentially as described earlier 50 . Alcohol dehydrogenase class III (adh3) and Ubiqutin (Ubq) genes were used as internal controls for Arachis diogoi and tobacco samples respectively 53,54 . The ΔΔC T method was used for the estimation of relative fold change in RNA expression.…”

Section: Analysis Of Cdna and Protein Sequence Basic Sequences Were mentioning

confidence: 99%

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

Singh

Shukla

Kirti

2020

Sci Rep

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cBL interacting protein kinases play important roles in adaptation to stress conditions. in the present study, we isolated a CBL-interacting protein kinase homolog (AdCIPK5) from a wild peanut (Arachis diogoi) with similarity to AtCIPK5 of Arabidopsis. Expression analyses in leaves of the wild peanut showed AdCIPK5 induction by exogenous signaling molecules including salicylic acid, abscisic acid and ethylene or abiotic stress factors like salt, PEG and sorbitol. The recombinant AdCIPK5-GFP protein was found to be localized to the nucleus, plasma membrane and cytoplasm. We overexpressed AdCIPK5 in tobacco plants and checked their level of tolerance to biotic and abiotic stresses. While wild type and transgenic plants displayed no significant differences to the treatment with the phytopathogen, Phytophthora parasitica pv nicotianae, the expression of AdCIPK5 increased salt and osmotic tolerance in transgenic plants. Analysis of different physiological parameters revealed that the transgenic plants maintained higher chlorophyll content and catalase activity with lower levels of H 2 o 2 and MDA content during the abiotic stress conditions. AdCIPK5 overexpression also contributed to the maintenance of a higher the K + /na + ratio under salt stress. The enhanced tolerance of transgenic plants was associated with elevated expression of stress-related marker genes; NtERD10C, NtERD10D, NtNCED1, NtSus1, NtCAT and NtSOS1. Taken together, these results indicate that AdCIPK5 is a positive regulator of salt and osmotic stress tolerance. Plants have developed an elaborate network of signaling pathways to counter the challenges posed by various stressful environmental conditions. In general, Ca 2+ serves as an ubiquitous secondary messenger that is reported to be involved in several biological processes including abiotic stress responses, pathogen defense and ion homeostasis adjustment 1. Studies on Ca 2+ dynamics have indicated stimulus-specific elevations in cytosolic Ca 2+ concentration, termed as 'calcium signatures'. To decode each signature, cells retain precise tools and mechanisms that include Ca 2+ sensors and their downstream target proteins 2. The sensor proteins exhibit a Ca 2+ binding site in their helix-loop-helix region 3. These proteins are classified into two categories as sensor responders and sensor relays 4. Sensor responders such as Ca 2+ dependent protein kinases (CDPKs) contain both Ca 2+ binding and kinase activities, while sensor relays like calmodulin (CaM) and calmodulin-like proteins (CML) do not exhibit kinase activity. However, after binding with Ca 2+ , they interact with other protein kinases to regulate their activities 5. Compared to other organisms, the Ca 2+ signaling mechanism in plants is more complex with the acquisition of several specific Ca 2+ sensor proteins. The calcineurin B-like protein (CBL) family is one of these specific sensor proteins. Arabidopsis CBL4/SOS3 is the first CBL protein reported from the plant system 6. Since then, several CBL proteins have been identified from diff...

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Section: Analysis Of Cdna and Protein Sequence Basic Sequences Were mentioning

confidence: 99%

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

Singh

Shukla

Kirti

2020

Sci Rep

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“…Many reports suggest that VPE is involved in many different types of PCD ( Fig. 2): (1) PCD induced by biotic stress caused by pathogens, elicitors or toxins (Hatsugai et al, 2004;Rojo et al, 2004;Iakimova et al, 2013;Sueldo et al, 2014;Kumar et al, 2015;Santana et al, 2016;Cai et al, 2018); (2) PCD induced by abiotic stresses such as heat, hydrogen peroxide, osmotic changes and aluminium (Danon et al, 2004;Yamada et al, 2004;Deng et al, 2011;Li et al, 2012;Kariya et al, 2013;Mendes et al, 2013;Zhang et al, 2013;Kim et al, 2014;Ran et al, 2014); and (3) PCD in developmental stages, including seed coat formation, lateral root emergence, aleurone cell degradation, pollen self-incompatibility, and leaf and flower petal senescence (Linnestad et al, 1998;Kinoshita et al, 1999;Nakaune et al, 2005;Yamada et al, 2009;Bosch et al, 2010;Ariizumi et al, 2011;Radchuk et al, 2011;Rocha et al, 2013;Shibuya et al, 2014;Zheng et al, 2017). VPE-dependent PCD has been reviewed in detail in a review article by Hatsugai et al (2015).…”

Section: Vpe Is Required For Some Types Of Pcd In Plantsmentioning

confidence: 99%

Vacuolar processing enzymes in the plant life cycle

et al. 2019

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Summary Vacuolar processing enzyme (VPE) is a cysteine‐type endopeptidase that has a substrate‐specificity for asparagine or aspartic acid residues and cleaves peptide bonds at their carboxyl‐terminal side. Various vacuolar proteins are synthesized as larger proprotein precursors, and VPE is an important initiator of maturation and activation of these proteins. It mediates programmed cell death (PCD) by provoking vacuolar rupture and initiating the proteolytic cascade leading to PCD. Vacuolar processing enzyme also possesses a peptide ligation activity, which is responsible for producing cyclic peptides in several plant species. These unique functions of VPE support developmental and environmental responses in plants. The number of VPE homologues is higher in angiosperm species, indicating that there has been differentiation and specialization of VPE function over the course of evolution. Angiosperm VPEs are separated into two major types: the γ‐type VPEs, which are expressed mainly in vegetative organs, and the β‐type VPEs, whose expression occurs mainly in storage organs; in eudicots, the δ‐type VPEs are further separated within γ‐type VPEs. This review also considers the importance of processing and peptide ligation by VPE in vacuolar protein maturation.

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“…The vectors were mobilized into Agrobacterium strain LBA4404 using freeze thaw method. Agrobacterium strains harboring different binary vector constructs were grown in Luria Broth in the presence of respective antibiotics, pelleted, resuspended and infiltrated as described earlier [18][19][20]. After the observation of cell death phenotype, the samples were collected, quick-frozen in liquid nitrogen and stored at À80°C for further analysis.…”

Section: Agro-infiltration Chemical Treatment and Cell Death Assessmentioning

confidence: 99%

A novel zinc‐binding alcohol dehydrogenase 2 from Arachis diogoi, expressed in resistance responses against late leaf spot pathogen, induces cell death when transexpressed in tobacco

et al. 2016

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A novel zinc‐binding alcohol dehydrogenase 2 (AdZADH2) was significantly upregulated in a wild peanut, Arachis diogoi treated with conidia of late leaf spot (LLS) pathogen, Phaeoisariopsis personata. This upregulation was not observed in a comparative analysis of cultivated peanut, which is highly susceptible to LLS. This zinc‐binding alcohol dehydrogenase possessed a Rossmann fold containing NADB domain in addition to the MDR domain present in all previously characterized plant ADH genes/proteins. Transient over‐expression of AdZADH2 under an estradiol inducible promoter (XVE) resulted in hypersensitive response (HR)‐like cell death in tobacco leaf. However, the same level of cell death was not observed when the domains were transiently expressed individually. Cell death observed in tobacco was associated with overexpression of cell death related proteins, antioxidative enzymes such as SOD, CAT and APX and pathogenesis‐related (PR) proteins. In A. diogoi, AdZADH2 expression was significantly upregulated in response to the plant signaling hormones salicylic acid, methyl jasmonate, and sodium nitroprusside.

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Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata

Cited by 16 publications

References 68 publications

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

Vacuolar processing enzymes in the plant life cycle

A novel zinc‐binding alcohol dehydrogenase 2 from Arachis diogoi, expressed in resistance responses against late leaf spot pathogen, induces cell death when transexpressed in tobacco

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