Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel

Abstract

Adult stem cells are of particular interest for the therapeutic approach in the field of regenerative medicine. Due to their ease of harvest, adipose-derived mesenchymal stem cells (ASCs) are an attractive stem cell source that has become increasingly popular. Critical aspects of applied cell therapies are the circumstances of transport from the laboratory towards the site of operation and cell delivery into the desired area. With regard to these issues, agarose-hydrogel was analyzed as a cell carrier matrix of equine and canine ASCs in vitro, which can be used for minimally invasive application. Isolated ASCs were expanded and 2.5 × 10(6) cells were combined with agarose-hydrogel to build a 0.4% hydrogel-cell solution which was stored at two temperatures (room temperature (RT) vs. 37 °C). Cell viability was investigated (live-dead assay) at different time points (0, 1, 6 and 24 h) in order to determine i) the effect of different temperatures on the cell survival as well as ii) the maximum possible time span before implantation. CFU-assay and WST-1 assay were performed after 24 h incubation in agarose-hydrogel and the cells were induced into adipogenic and osteogenic differentiation to analyze the effects of the incubation on the cell behaviour. No negative effect of the agarose-hydrogel incubation was determined on the different species' cell behaviour at either RT or 37 °C with any of the assays used. We can recommend agarose-hydrogel as a cell carrier for cell implantation with a storage period of up to 24 h at room temperature or at 37 °C prior to implantation.

Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells

“…Chondrogenic differentiation of canine MSCs, particularly, has been shown to be problematic [7,8] and has often omitted from published characterization and differentiation assays [36,37,38,39]. Although reasons for this poor chondrogenic differentiation is unclear, cell senescence and the sudden change in culture conditions to high cell density pellet culture with low nutrient and low oxygen [40] have been shown to influence the ability of some cell types to proliferate and/or differentiate.…”

In vitro ovarian cancer model based on three-dimensional agarose hydrogel

“…On the other hand, agarose hydrogel can well support cell proliferation and maintain the phenotype in vitro, which has been well demonstrated in a great number of tissue engineering studies. 23–39 Most importantly, it can enhance the secretion of extracellular matrix (ECM) by supplying an appropriate environment for a stable cell phenotype. 33 Besides, it is easy to be reproducibly manufactured, convenient to be handled, and amenable to large-scale use.…”

The metabolic syndrome alters the miRNA signature of porcine adipose tissue‐derived mesenchymal stem cells

“…Furthermore, these cells possess important pro-angiogenic, immunomodulatory, and antiinflammatory properties, and can be isolated from multiple tissues, including bone marrow, adipose, peripheral blood and skeletal muscle. The fat tissue in most species studied yields large numbers of MSC (2). Human, mouse, and porcine MSC seem to share a similar phenotype, although characterizing MSC from large animal species is somewhat limited by a lack of species-specific antibodies (3).…”