Characterization of an Attenuated Temperature Sensitive Feline Infectious Peritonitis Vaccine Virus

Gerber, J D; Pfeiffer, N. E.; Ingersoll, J D; Christianson, K K; Landon, R. M.; Selzer, N.L.; Beckenhauer, W H

doi:10.1007/978-1-4684-5823-7_67

Cited by 7 publications

(7 citation statements)

References 8 publications

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“…Consensus between the two sequences is shown by an asterisk. The position of amino acid changes resulting in resistance to neutralization is shown by an arrowhead for FIPV and a solid circle for TGEV (10,12). The proposed FIPV antigenic sites A1 and A2 are indicated.…”

Section: Discussionmentioning

confidence: 99%

“…Attempts have been made to develop a vaccine against FIPV by immunizing cats with FIPV antigen that does not contain S (26) or with virus that produces only low levels of neutralizing antibodies (11,12). Both strategies have been attempts to avoid the production of antibodies that appear to be inseparably linked to both neutralization and ADE.…”

Section: Discussionmentioning

confidence: 99%

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Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement

Corapi

Darteil

Audonnet

et al. 1995

J Virol

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The S glycoprotein of feline infectious peritonitis virus (FIPV) has been shown to contain the antigenic sites responsible for eliciting both neutralization and antibody-dependent enhancement. To determine the region of S responsible, overlapping DNA fragments spanning the entire S gene were cloned and expressed as fusion proteins by in vitro transcription and translation. Fusion proteins containing relevant epitopes were identified by radioimmunoprecipitation with neutralizing and enhancing FIPV-specific monoclonal antibodies (MAbs). A region spanning residues 509 to 673 reacted with most MAbs tested. Translation in the presence of microsomal membranes did not enhance reactivity, suggesting that glycosylation is not essential for recognition by the MAbs. To localize the antigenic sites further, several MAb-resistant (mar) mutants of FIPV were cloned and sequenced. Amino acid residues that contribute to the neutralizing and enhancing epitopes were localized to two regions, designated A1 and A2, which show partial overlap with the homologous antigenic site A of transmissible gastroenteritis virus. Site A1 contains residues 568 and 591 and is homologous with part of subsite Aa of transmissible gastroenteritis virus. Site A2 contains residues 643, 649, and 656. Double mutations in sites A1 and A2 were found in mar mutants derived from neutralizing and enhancing MAbs 23F4.5 and 18A7.4, while a single mutation in site A2 was found in a mar mutant derived from MAb 24H5.4, which is neutralizing but not enhancing. The data suggest that site A2, which includes residues 643 to 656, is a dominant neutralizing site of FIPV and that sites A1 and A2 may act in concert to induce antibody-dependent enhancement.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement

Corapi

Darteil

Audonnet

et al. 1995

J Virol

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“…Nevertheless, a vaccine was licensed (Primucell, Pfizer Animal Health) incorporating a temperature-sensitive mutant of the FCoV strain DF2-FIPV, which can replicate in the cool lining of the upper respiratory tract but not at higher internal body temperatures [172][173][174][175]. This vaccine, administered intranasally, produces local immunity (IgA antibodies) at the site where FCoV first enters the body (the oropharynx) and also induces cell-mediated immunity.…”

Section: Vaccinationmentioning

confidence: 99%

Feline infectious peritonitis

Hartmann

2005

Veterinary Clinics of North America: Small Animal Practice

148

251

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The article discusses feline infectious peritonitis (FIP), an important disease frequently seen in veterinary practice. FIP causes many problems to the veterinarian as it can be difficult to definitively diagnose the disease, as there is no effective treatment, and as prophylactic interventions are not very successful. Although intense research has created a lot of new knowledge about this disease in the last years, there are still many unanswered questions. The objective of this article is to review recent knowledge and to increase understanding of the complex pathogenesis of FIP.

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“…However, the S gene of these viruses is a likely target for variablity. The S gene from FIPV 79-1146 has been sequenced and shown that when compared to the related coronavirus TGEV, the heterogeneity is confined to the N-terminal 274 aa [9,10]. Feline enteric coronavirus and FIPV may also differ in their N-termini.…”

Section: Discussionmentioning

confidence: 99%

“…Symptoms such as febrile response, lymphopenia, leukopenia, icterus and decreased packed cell volume were all evaluated. The total clinical score was the sum of each clinical score for the duration of the study [10]. Cats with a total clinical score of less than i0 were designated as non-symptomatic (NS).…”

Section: Clinical Evaluation Of Catsmentioning

confidence: 99%

Serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides andE. coli fusion protein

Suiter

Pfeiffer

Jones

et al. 1995

Archives of Virology

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Cats exposed to feline infectious peritonitis virus (FIPV) or feline enteric coronavirus (FECV) cannot be differentiated by serological analysis. Three synthetic peptides and an E. coli recombinant fusion protein generated from FIPV 79-1146 spike gene sequence were produced. Coronavirus positive cat sera reacted to peptide aa 950-990 but were non-reactive to aa137-151 and aa 150-180 peptides as demonstrated by ELISA. Amino acid sequence 97-222 expressed as a galk fusion protein in E. coli was tested against coronavirus positive cat sera by western blot analysis. Only sera from cats exposed to the FIPV type-II strains DF-2 or 79-1146 that were exhibiting signs of FIP recognized the fusion protein. Sera from FECV exposed cats did not recognize the 97-222 fusion protein in western blot analysis.

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Characterization of an Attenuated Temperature Sensitive Feline Infectious Peritonitis Vaccine Virus

Cited by 7 publications

References 8 publications

Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement

Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement

Feline infectious peritonitis

Serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides andE. coli fusion protein

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