Characterization of Aptamer–Ligand Complexes

Mapping the gaps in chemical analysis for the characterisation of aptamer-target interactions

“…equilibrium dialysis, chromatography approaches and gel-based methods) that can be used for studying aptamers. These classical methods were previously discussed by Mirando-Castro et al [23]. First, techniques to retrieve kinetic and thermodynamic information on the binding event are discussed.…”

Impedimetric aptamer-based glycan PSA score for discrimination of prostate cancer from other prostate diseases

“…Fernández et al, 2020). However, previous studies have indicated that the interaction aptamer-protein depends on which partner has been immobilized due to surface interactions (Amaya-González et al, 2015;Daniel et al, 2013;Miranda-Castro et al, 2018). The immobilization of the aptamers onto the sensing surface could change their conformation and their ability to bind to the protein.…”

Chromatin regulators-related lncRNA signature predicting the prognosis of kidney renal clear cell carcinoma and its relationship with immune microenvironment: A study based on bioinformatics and experimental validation

“…Long noncoding RNAs (LncRNAs), a class of no protein genes coding potential, are initially assumed to be nonfunctional transcriptional byproducts. Studies on transcriptional activation, cell cycle regulation and epigenetic regulation (Miranda-Castro et al, 2019;Zhang et al, 2019) have been found for lncRNAs in the onset and development of disease. The occurrence and progression of tumors are influenced by more than a hundred lncRNAs with dysregulated expression, particularly urinary malignancies, as research advances.…”