Characterization of Bacillus thuringiensis subsp. tohokuensis CAB167 Isolate against Mosquito Larva

Abstract

Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. LC 50 values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.

Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.

“…thuringiensis from B . cereus ( Kil et al, 2008 ; Whiteley and Schnepf, 1986 ). Therefore, the formation of endospores and parasporal inclusions of the bacterial isolates were observed by phase-contrast microscopy.…”

“…Bacillus thuringiensis has been used as a biological control agent for insect pests in agriculture due to its parasporal inclusions, which often contain one or more insecticidal proteins (de Almeida Melo et al, 2016;Dulmage, 1970;Kil et al, 2008;Lacey et al, 2015;Wasano et al, 1998). In addition to its properties as a biopesticide, ongoing attention is being paid to the use of B. thuringiensis to control plant pathogenic fungi (Jouzani et al, 2017;Zhou et al, 2008).…”

“…To observe the endospores and parasporal inclusions of bacterial isolates, the isolates were grown on Nutrient agar (Difco Laboratories) for 2 days at 30°C in the dark. Bacterial samples were suspended in SDW on a microscope slide, covered with a coverslip, and observed under a phase-contrast microscopy (Axio Image A2, Carl Zeiss, Jena, Germany) at 1,000× magnification with immersion oil ( Kil et al, 2008 ; Kim et al, 1995 ).…”

“…The 16S rRNA gene Phase-contrast microscopic examination. Bacillus thuringiensis is a gram-positive rod-shaped bacterium characterized by the production of parasporal inclusions, which differentiates B. thuringiensis from B. cereus (Kil et al, 2008;Whiteley and Schnepf, 1986). Therefore, the formation of endospores and parasporal inclusions of the bacterial isolates were observed by phase-contrast microscopy.…”

Control Effects against Mosquitoes Larva of Bacillus thuringiensis subsp. israelensis CAB199 isolate according to Different Formulations

“…농약을 이용한 화학적 방제에 의존하여 왔는데 (Kil et al, 2008) (Ree et al, 1988 (Kil et al, 2008). 곤충생장조절제 중에서 키틴합성 저해기작을 가진 Novaluron과 탈피촉진기작을 보이는 Methofenozide는 다른 작용기작을 가진 곤충생장조절제보 다 모기유충방제에 높은 활성을 보이는 것으로 확인되었으 며 (Choi et al, 2007), 최근 전세계적으로 모기유충을 방제 하는데 이용되고 있다(Arredondo-Jimenez and Valdez-Delgado, 2006; Beckage et al, 2004).…”